DR406093

Overview
NameDR406093
Unique NameDR406093
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length753
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HSP12_DAUCA (18.0 kDa class I heat shock protein OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 2.858e-47
Identity = 94/159 (59.12%), Postives = 114/159 (71.70%), Query Frame = 1
Query:   76 MSLIPSIFGGRRTNVFDPVSLDVWDPFDGF-LSSALTNAPSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            MS+IPS FG RR+NV +P SLD+WDPF  + L ++   +    +ET+ FAN  IDWKETPQAHVFKADLPGL+          G++L ISGER+KE+EEKN+KWHRVE SSGKFLRRFRLP+NA V++VKA MENGVLTVT            +I ISG
Sbjct:    1 MSIIPSFFGSRRSNVLNPFSLDIWDPFQDYPLITSSGTSSEFGKETAAFANTHIDWKETPQAHVFKADLPGLKKEEVKVEVEEGKVLQISGERNKEKEEKNNKWHRVEFSSGKFLRRFRLPENANVDEVKAGMENGVLTVTVPKVEMKKPEVKSIHISG 159          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HS17A_ORYSJ (17.9 kDa class I heat shock protein OS=Oryza sativa subsp. japonica GN=HSP17.9A PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 8.315e-47
Identity = 100/157 (63.69%), Postives = 113/157 (71.97%), Query Frame = 1
Query:  106 RRTNVFDPVSLDVWDPFDGFL-----SSALTNAPSSAR----ETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            RR+NVFDP SLD+WDPFDGF      SS+ +  PS  R    ET+ FA ARIDWKETP+AHVFKAD+PGL+          G IL ISGER+KEQEEK D+WHRVERSSGKFLRRFRLPDNAK EQ+KASMENGVLTVT            +I+ISG
Sbjct:    5 RRSNVFDPFSLDLWDPFDGFPFGSGGSSSGSIFPSFPRGASSETAAFAGARIDWKETPEAHVFKADVPGLKKEEVKVEVDDGNILQISGERNKEQEEKTDQWHRVERSSGKFLRRFRLPDNAKPEQIKASMENGVLTVTVPKEEAKKPDVKSIQISG 161          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HS16B_ORYSJ (16.9 kDa class I heat shock protein 2 OS=Oryza sativa subsp. japonica GN=HSP16.9B PE=2 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 8.315e-47
Identity = 92/148 (62.16%), Postives = 111/148 (75.00%), Query Frame = 1
Query:  106 RRTNVFDPVSLDVWDPFDGFLSSALTNAPSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            RR+NVFDP SLD+WDPFD    S +    +S  +T+ FANARIDWKETP++HVFKADLPG++          G +L+ISG+RSKE+E+KNDKWHRVERSSG+F+RRFRLP+NAKV+QVKA MENGVLTVT            AIEISG
Sbjct:    5 RRSNVFDPFSLDLWDPFDSVFRSVVP--ATSDNDTAAFANARIDWKETPESHVFKADLPGVKKEEVKVEVEEGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQVKAGMENGVLTVTVPKAEVKKPEVKAIEISG 150          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HS16A_ORYSJ (16.9 kDa class I heat shock protein 1 OS=Oryza sativa subsp. japonica GN=HSP16.9A PE=1 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.852e-46
Identity = 91/148 (61.49%), Postives = 111/148 (75.00%), Query Frame = 1
Query:  106 RRTNVFDPVSLDVWDPFDGFLSSALTNAPSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            RR+NVFDP SLD+WDPFD    S +    +S  +T+ FANARIDWKETP++HVFKADLPG++          G +L+ISG+RSKE+E+KNDKWHRVERSSG+F+RRFRLP+NAKV+QVKA +ENGVLTVT            AIEISG
Sbjct:    5 RRSNVFDPFSLDLWDPFDSVFRSVVP--ATSDNDTAAFANARIDWKETPESHVFKADLPGVKKEEVKVEVEEGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQVKAGLENGVLTVTVPKAEVKKPEVKAIEISG 150          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HSP15_SOYBN (17.6 kDa class I heat shock protein OS=Glycine max GN=HSP17.6-L PE=3 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 3.160e-46
Identity = 96/160 (60.00%), Postives = 112/160 (70.00%), Query Frame = 1
Query:   76 MSLIPSIFGGRRTNVFDPVSLDVWDPFDGFLSSALTNAPSSA--RETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            MSLIPSIFGG R+NVFDP SLD+WDPF  F      + P+S+   E S F N R+DWKET +AHV KAD+PGL+           R+L ISGER+ E+E+KND WHRV+RSSGKF+RRFRLP+NAKVEQVKA MENGVLTVT             IEISG
Sbjct:    1 MSLIPSIFGGPRSNVFDPFSLDMWDPFKDF------HVPTSSVSAENSAFVNTRVDWKETQEAHVLKADIPGLKKEEVKVQIEDDRVLQISGERNVEKEDKNDTWHRVDRSSGKFMRRFRLPENAKVEQVKACMENGVLTVTIPKEEVKKSDVKPIEISG 154          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HSP12_SOLPE (17.6 kDa class I heat shock protein OS=Solanum peruvianum PE=2 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 3.160e-46
Identity = 99/159 (62.26%), Postives = 115/159 (72.33%), Query Frame = 1
Query:   76 MSLIPSIFGGRR-TNVFDPVSLDVWDPFDGFLSSALTNAPSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            MSLIP IFG RR T+VFDP S+DV+DPF       L    S++ ETS FAN RIDWKETP+AHVFKADLPGL+           R+L ISGER+ E+E+KND WHRVERSSGKF+RRFRLP+NAK++QVKASMENGVLTVT            +IEISG
Sbjct:    1 MSLIPRIFGDRRSTSVFDPFSIDVFDPF-----KELGFTVSNSGETSAFANTRIDWKETPEAHVFKADLPGLKKEEVKVEVEEDRVLQISGERNVEKEDKNDTWHRVERSSGKFMRRFRLPENAKMDQVKASMENGVLTVTVPKEEVNNPDVKSIEISG 154          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HSP11_HELAN (17.6 kDa class I heat shock protein OS=Helianthus annuus GN=HSP17.6 PE=2 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 5.390e-46
Identity = 88/139 (63.31%), Postives = 106/139 (76.26%), Query Frame = 1
Query:   76 MSLIPSIFGGRRTNVFDPVSLDVWDPFDGFLSSALTNAPSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTV 492
            MS+IPS F  +R+N+FDP SLD WDPF G +S+        ARET+   NARIDWKETP+AHV KADLPG++          GR+L ISGER +EQEEK+D WHRVERSSGKF+RRFRLP+NAK+++VKA MENGVLTV
Sbjct:    1 MSIIPSFFTSKRSNIFDPFSLDTWDPFQGIIST------EPARETAAIVNARIDWKETPEAHVLKADLPGMKKEEVKVEVEDGRVLQISGERCREQEEKDDTWHRVERSSGKFIRRFRLPENAKMDEVKAMMENGVLTV 133          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HS16C_ORYSJ (16.9 kDa class I heat shock protein 3 OS=Oryza sativa subsp. japonica GN=HSP16.9C PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 7.039e-46
Identity = 92/148 (62.16%), Postives = 112/148 (75.68%), Query Frame = 1
Query:  106 RRTNVFDPVSLDVWDPFDGFLSSALTNAPSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            RR+NVFDP + D WDPFDG   S +    +S R+T+ FANAR+DWKETP++HVFKADLPG++          G +L+ISG+RSKE+E+KNDKWHRVERSSG+F+RRFRLP+NAKV+QVKASMENGVLTVT            AIEISG
Sbjct:    5 RRSNVFDPFA-DFWDPFDGVFRSLVP--ATSDRDTAAFANARVDWKETPESHVFKADLPGVKKEEVKVEVEEGNVLVISGQRSKEKEDKNDKWHRVERSSGQFMRRFRLPENAKVDQVKASMENGVLTVTVPKAEVKKPEVKAIEISG 149          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HS181_ORYSJ (18.1 kDa class I heat shock protein OS=Oryza sativa subsp. japonica GN=HSP18.1 PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 9.194e-46
Identity = 93/157 (59.24%), Postives = 110/157 (70.06%), Query Frame = 1
Query:  106 RRTNVFDPVSLDVWDPFDGFLSSALTNAPSS---------ARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            RR+NVFDP SLD+WDPFDGF   + + +  S         + ET+ FA ARIDWKETP+AHVFKAD+PGL+          G +L ISGERSKEQEEK DKWHRVERSSGKFLRRFRLP+N K EQ+KASMENGVLTVT            +I+++G
Sbjct:    5 RRSNVFDPFSLDLWDPFDGFPFGSGSRSSGSIFPSFPRGTSSETAAFAGARIDWKETPEAHVFKADVPGLKKEEVKVEVEDGNVLQISGERSKEQEEKTDKWHRVERSSGKFLRRFRLPENTKPEQIKASMENGVLTVTVPKEEPKKPDVKSIQVTG 161          
BLAST of DR406093 vs. ExPASy Swiss-Prot
Match: HS174_ORYSJ (17.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica GN=HSP17.4 PE=2 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 3.494e-45
Identity = 91/150 (60.67%), Postives = 108/150 (72.00%), Query Frame = 1
Query:  106 RRTNVFDPVSLDVWDPFDGFLSSALTNA--PSSARETSQFANARIDWKETPQAHVFKADLPGLRXXXXXXXXXXGRILLISGERSKEQEEKNDKWHRVERSSGKFLRRFRLPDNAKVEQVKASMENGVLTVTXXXXXXXXXXXXAIEISG 549
            RR+NVFDP SLD+WDPFDGF   + + +  P +  + + FA ARIDWKETP+AHVFKAD+PGL+          G +L ISGER KEQEEK DKWHRVERSSGKFLRRFRLP+N K EQ+KASMENGVLTVT            +I+I+G
Sbjct:    5 RRSNVFDPFSLDLWDPFDGFPFGSGSGSLFPRANSDAAAFAGARIDWKETPEAHVFKADVPGLKKEEVKVEVEDGNVLQISGERIKEQEEKTDKWHRVERSSGKFLRRFRLPENTKPEQIKASMENGVLTVTVPKEEPKKPDVKSIQITG 154          
The following BLAST results are available for this feature:
BLAST of DR406093 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55
Match NameE-valueIdentityDescription
HSP12_DAUCA2.858e-4759.1218.0 kDa class I heat shock protein OS=Daucus caro... [more]
HS17A_ORYSJ8.315e-4763.6917.9 kDa class I heat shock protein OS=Oryza sativ... [more]
HS16B_ORYSJ8.315e-4762.1616.9 kDa class I heat shock protein 2 OS=Oryza sat... [more]
HS16A_ORYSJ1.852e-4661.4916.9 kDa class I heat shock protein 1 OS=Oryza sat... [more]
HSP15_SOYBN3.160e-4660.0017.6 kDa class I heat shock protein OS=Glycine max... [more]
HSP12_SOLPE3.160e-4662.2617.6 kDa class I heat shock protein OS=Solanum per... [more]
HSP11_HELAN5.390e-4663.3117.6 kDa class I heat shock protein OS=Helianthus ... [more]
HS16C_ORYSJ7.039e-4662.1616.9 kDa class I heat shock protein 3 OS=Oryza sat... [more]
HS181_ORYSJ9.194e-4659.2418.1 kDa class I heat shock protein OS=Oryza sativ... [more]
HS174_ORYSJ3.494e-4560.6717.4 kDa class I heat shock protein OS=Oryza sativ... [more]

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Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248L03 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248L03 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR406093 ID=DR406093; Name=DR406093; organism=Citrus sinensis; type=EST; length=753bp
GGGGGATCTTCAAATTGATTGATCAAAGCATTTCCGAAGTTGTTGAGCAT
TTAATTAGCGTCCCAGTTTGCCATAATGTCACTCATTCCAAGCATCTTCG
GTGGCCGAAGAACCAACGTGTTCGATCCAGTTTCATTAGATGTATGGGAT
CCTTTCGACGGGTTCTTGTCCTCTGCCCTTACCAACGCGCCGTCTTCGGC
TAGGGAAACATCCCAATTCGCCAATGCAAGAATTGACTGGAAAGAGACTC
CTCAGGCTCACGTATTCAAAGCTGATCTTCCAGGGCTGAGGAAAGAAGAA
GTAAAAGTTGAGGTTGAAGAGGGCAGGATTCTGCTGATAAGCGGAGAGAG
GAGCAAGGAGCAGGAAGAGAAGAATGACAAGTGGCATCGCGTCGAGAGGA
GCAGTGGCAAGTTCCTGAGGAGGTTCAGGTTGCCCGACAATGCTAAGGTG
GAACAGGTTAAGGCCAGCATGGAGAATGGTGTGCTCACAGTCACCGTTCC
TAAACAGGAGGAGAAGAAACCTGAAGTTAAGGCTATTGAGATCTCTGGCT
AAGTGATAACTGTAGATGTTTTCAAGATAGTGTCTTTGGTTTGCTAATAA
ATTTGGTACGAAAGCAAAGCCCTTGTGTGTTTGTATGTNGTGTGTNGTGT
TCGTTTTCTTCCCTTCCCCCTGTTTAATTTNNNGTGANNNTGTTTGTGTG
TTTGCAAGAGCTTCAGGAGCCAGTCTCAAGCGTTGTAACTAGTTTTTTAA
TGA
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