DR405962 | Citrus Genome Database

Overview


Name	DR405962
Unique Name	DR405962
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	729

Libraries


Library Name	Type
Mature Juice Sac from Navel Orange in pTriplEx2 vector	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE3_ARATH (UDP-glucose 4-epimerase 3 OS=Arabidopsis thaliana GN=At4g23920 PE=2 SV=1)

HSP 1 Score: 283.108 bits (723), Expect = 8.848e-78
Identity = 132/175 (75.43%), Postives = 157/175 (89.71%), Query Frame = 3

Query:  159 MAKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAGNQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEWK 683
            MAK++LVTGGAGYIGSHTVLQLL GGY  VVVDN DN+S  ++ +VK+LAG  G+ ++FH++DLRD+ ALE +F++TKF AVIHFAGL+AVGESV+KPLLY++NN++GT+TLLEVMA +GCKNLVFSSSATVYGWPK VPCTEE P+ A NPYGRTKLFIEEICRDVHRSDSEWK
Sbjct:    1 MAKSVLVTGGAGYIGSHTVLQLLEGGYSAVVVDNYDNSSAASLQRVKKLAGENGNRLSFHQVDLRDRPALEKIFSETKFDAVIHFAGLKAVGESVEKPLLYYNNNIVGTVTLLEVMAQYGCKNLVFSSSATVYGWPKEVPCTEESPISATNPYGRTKLFIEEICRDVHRSDSEWK 175

HSP 2 Score: 29.261 bits (64), Expect = 8.848e-78
Identity = 13/16 (81.25%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  682 KIILLRYFNPVXXXPS 729
            KIILLRYFNPV   PS
Sbjct:  175 KIILLRYFNPVGAHPS 190

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE2_ARATH (UDP-glucose 4-epimerase 2 OS=Arabidopsis thaliana GN=At4g10960 PE=2 SV=3)

HSP 1 Score: 282.722 bits (722), Expect = 1.154e-77
Identity = 132/175 (75.43%), Postives = 159/175 (90.86%), Query Frame = 3

Query:  159 MAKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAGNQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEWK 683
            MA+N+LV+GGAGYIGSHTVLQLLLGGY  VVVDNLDN+S +++ +VK+LA   G+ ++FH++DLRD++ALE +F++TKF AVIHFAGL+AVGESV+KPLLY++NNL+GTITLLEVMA HGCKNLVFSSSATVYG PK VPCTEEFP+ A+NPYGRTKLFIEEICRDV+ SD EWK
Sbjct:    2 MARNVLVSGGAGYIGSHTVLQLLLGGYSVVVVDNLDNSSAVSLQRVKKLAAEHGERLSFHQVDLRDRSALEKIFSETKFDAVIHFAGLKAVGESVEKPLLYYNNNLVGTITLLEVMAQHGCKNLVFSSSATVYGSPKEVPCTEEFPISALNPYGRTKLFIEEICRDVYGSDPEWK 176

HSP 2 Score: 29.261 bits (64), Expect = 1.154e-77
Identity = 13/16 (81.25%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  682 KIILLRYFNPVXXXPS 729
            KIILLRYFNPV   PS
Sbjct:  176 KIILLRYFNPVGAHPS 191

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE2_CYATE (UDP-glucose 4-epimerase GEPI48 OS=Cyamopsis tetragonoloba PE=2 SV=1)

HSP 1 Score: 280.026 bits (715), Expect = 7.410e-77
Identity = 134/174 (77.01%), Postives = 153/174 (87.93%), Query Frame = 3

Query:  162 AKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAGNQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEWK 683
            ++ +LVTGGAGYIGSHTVLQLLLGG+K VVVDNLDN+SE A+ +VKELAG    N++FHKLDLRD+ ALE +F+ TKF +VIHFAGL+AVGESVQKPLLY+DNNLIGTI L EVMAAHGCK LVFSSSATVYG PK VPCTEEFPL A NPYGRTKL IEEICRD++R++ EWK
Sbjct:    3 SQTVLVTGGAGYIGSHTVLQLLLGGFKAVVVDNLDNSSETAIHRVKELAGKFAGNLSFHKLDLRDRDALEKIFSSTKFDSVIHFAGLKAVGESVQKPLLYYDNNLIGTIVLFEVMAAHGCKKLVFSSSATVYGLPKEVPCTEEFPLSAANPYGRTKLIIEEICRDIYRAEQEWK 176

HSP 2 Score: 29.261 bits (64), Expect = 7.410e-77
Identity = 13/16 (81.25%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  682 KIILLRYFNPVXXXPS 729
            KIILLRYFNPV   PS
Sbjct:  176 KIILLRYFNPVGAHPS 191

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE1_PEA (UDP-glucose 4-epimerase OS=Pisum sativum GN=GALE PE=2 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 4.998e-57
Identity = 105/175 (60.00%), Postives = 135/175 (77.14%), Query Frame = 3

Query:  162 AKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAG-NQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEWK 683
            ++ ILVTG AG+IG+HTV+QLL  G+   ++DN DN+   AV +V+E+ G N   N+ F   DLR+K  LE LF+K+KF AVIHFAGL+AVGESV+ P  YFDNNL+GTI L EVMA H CK +VFSSSATVYG P+ +PC E+F L+AMNPYGRTKLF+EEI RD+ +++ EW+
Sbjct:    5 SQKILVTGSAGFIGTHTVVQLLNNGFNVSIIDNFDNSVMEAVERVREVVGSNLSQNLEFTLGDLRNKDDLEKLFSKSKFDAVIHFAGLKAVGESVENPRRYFDNNLVGTINLYEVMAKHNCKKMVFSSSATVYGQPEKIPCVEDFKLQAMNPYGRTKLFLEEIARDIQKAEPEWR 179

HSP 2 Score: 24.6386 bits (52), Expect = 4.998e-57
Identity = 9/11 (81.82%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  682 KIILLRYFNPV 714
            +I+LLRYFNPV
Sbjct:  179 RIVLLRYFNPV 189

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE1_ARATH (UDP-glucose 4-epimerase 1 OS=Arabidopsis thaliana GN=At1g12780 PE=1 SV=2)

HSP 1 Score: 215.698 bits (548), Expect = 2.454e-56
Identity = 104/177 (58.76%), Postives = 137/177 (77.40%), Query Frame = 3

Query:  156 SMAKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAG-NQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEWK 683
            S+ +NILVTGGAG+IG+HTV+QLL  G+K  ++DN DN+   AV +V+EL G +    + F+  DLR+K  +E LF+K +F AVIHFAGL+AVGESV+ P  YFDNNL+GTI L E MA + CK +VFSSSATVYG P+ +PC E+F L+AMNPYGRTKLF+EEI RD+ +++ EW+
Sbjct:    4 SVEQNILVTGGAGFIGTHTVVQLLKDGFKVSIIDNFDNSVIEAVDRVRELVGPDLSKKLDFNLGDLRNKGDIEKLFSKQRFDAVIHFAGLKAVGESVENPRRYFDNNLVGTINLYETMAKYNCKMMVFSSSATVYGQPEKIPCMEDFELKAMNPYGRTKLFLEEIARDIQKAEPEWR 180

HSP 2 Score: 25.0238 bits (53), Expect = 2.454e-56
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  682 KIILLRYFNPV 714
            +IILLRYFNPV
Sbjct:  180 RIILLRYFNPV 190

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE1_CYATE (UDP-glucose 4-epimerase GEPI42 OS=Cyamopsis tetragonoloba PE=2 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 8.254e-51
Identity = 100/183 (54.64%), Postives = 130/183 (71.04%), Query Frame = 3

Query:  138 LVSPLDSMAKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAGNQ-GDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEWK 683
            +VS   +  + ILVTGGAG+IGSHTV+QLL  G+   ++DNL N+   AV +V+ L G     N+ FH  DLR+   L+ LF+KTKF AVIHFAGL+ VGESV  P  Y+DNNL+ TI L +VM+   CK LV SSSATVYG P  +PC E+  L AMNPYGR+KLF+EE+ RD+ R+++EW+
Sbjct:    1 MVSSRMASGETILVTGGAGFIGSHTVVQLLKQGFHVSIIDNLYNSVIDAVHRVRLLVGPLLSSNLHFHHGDLRNIHDLDILFSKTKFDAVIHFAGLKGVGESVLNPSNYYDNNLVATINLFQVMSKFNCKKLVISSSATVYGQPDQIPCVEDSNLHAMNPYGRSKLFVEEVARDIQRAEAEWR 183

HSP 2 Score: 25.0238 bits (53), Expect = 8.254e-51
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  682 KIILLRYFNPV 714
            +IILLRYFNPV
Sbjct:  183 RIILLRYFNPV 193

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE_BACSU (UDP-glucose 4-epimerase OS=Bacillus subtilis GN=galE PE=3 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 1.076e-50
Identity = 99/178 (55.62%), Postives = 131/178 (73.60%), Query Frame = 3

Query:  171 ILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAGNQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEWKDHIAEIF 704
            ILVTGGAGYIGSHT ++LL  GY+ VV+DNL N+S  A+ +VKE+ G    ++TF++ DL D+ A++++FA+ +  AVIHFAGL+AVGESV  PL Y+ NNL GT  L E M  +G K +VFSSSATVYG P+  P TE+FPL A NPYG+TKL +E+I RD+H +D+EW   +   F
Sbjct:    3 ILVTGGAGYIGSHTCVELLNSGYEIVVLDNLSNSSAEALNRVKEITGK---DLTFYEADLLDREAVDSVFAENEIEAVIHFAGLKAVGESVAIPLKYYHNNLTGTFILCEAMEKYGVKKIVFSSSATVYGVPETSPITEDFPLGATNPYGQTKLMLEQILRDLHTADNEWSVALLRYF 177

HSP 2 Score: 23.0978 bits (48), Expect = 1.076e-50
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  685 IILLRYFNPVXXXPS 729
            + LLRYFNP    PS
Sbjct:  171 VALLRYFNPFGAHPS 185

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE_DICDI (UDP-glucose 4-epimerase OS=Dictyostelium discoideum GN=galE PE=1 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.654e-48
Identity = 95/180 (52.78%), Postives = 128/180 (71.11%), Query Frame = 3

Query:  141 VSPLDSMAKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASEIAVAKVKELAGNQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPLEAMNPYGRTKLFIEEICRDVHRSDSEW 680
            + P+D     I+VTGGAGYIGSHTV++L+  GY  V+VDNL N+S  A+ +V+ + G +   + FH +D+ ++ AL+ +F      +VIHFAGL+AVGES + PL Y++NN+ GT+TLL +M  H  K LVFSSSATVYG P  VP TE+FPL A NPYGRTKL++E I +D+  SD EW
Sbjct:    1 MEPIDD---RIMVTGGAGYIGSHTVIELIEAGYTPVIVDNLSNSSLEAIKRVESITGKE---IEFHHVDIMNEKALDEIFETGNIRSVIHFAGLKAVGESNKLPLKYYNNNIAGTLTLLNLMDKHRVKKLVFSSSATVYGDPHTVPITEDFPLSATNPYGRTKLYVEGILQDLCASDPEW 174

HSP 2 Score: 25.0238 bits (53), Expect = 1.654e-48
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 1

Query:  688 ILLRYFNPVXXXPS 729
            I+LRYFNPV   PS
Sbjct:  177 IMLRYFNPVGAHPS 190

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE_MOUSE (UDP-glucose 4-epimerase OS=Mus musculus GN=Gale PE=2 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 8.799e-47
Identity = 102/187 (54.55%), Postives = 129/187 (68.98%), Query Frame = 3

Query:  165 KNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASE------IAVAKVKELAGNQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPL-EAMNPYGRTKLFIEEICRDVHRSDSEWKDHIAEIF 704
            + +LVTGGAGYIGSHTVL+LL  GY  VV+DN  NA         ++ +V+EL G    ++ F ++D+ D+AAL+ LF K  F AVIHFAGL+AVGESVQKPL Y+  NL GTI LLE+M AHG KNLVFSSSATVYG P+ +P  E  P     NPYG++K FIEE+ RD+ R+D+ W   +   F
Sbjct:    2 EKVLVTGGAGYIGSHTVLELLEAGYSPVVIDNFHNAIRGEDSMPESLRRVQELTGR---SVEFEEMDILDQAALQHLFKKHSFKAVIHFAGLKAVGESVQKPLDYYRVNLTGTIQLLEIMRAHGVKNLVFSSSATVYGNPQYLPLDEAHPTGGCTNPYGKSKFFIEEMIRDLCRADTAWNAVLLRYF 185

HSP 2 Score: 21.9422 bits (45), Expect = 8.799e-47
Identity = 7/9 (77.78%), Postives = 9/9 (100.00%), Query Frame = 1

Query:  688 ILLRYFNPV 714
            +LLRYFNP+
Sbjct:  180 VLLRYFNPI 188

[Close]

BLAST of DR405962 vs. ExPASy Swiss-Prot
Match: GALE_PONAB (UDP-glucose 4-epimerase OS=Pongo abelii GN=GALE PE=2 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.731e-46
Identity = 102/189 (53.97%), Postives = 129/189 (68.25%), Query Frame = 3

Query:  159 MAKNILVTGGAGYIGSHTVLQLLLGGYKTVVVDNLDNASE------IAVAKVKELAGNQGDNMTFHKLDLRDKAALEALFAKTKFGAVIHFAGLEAVGESVQKPLLYFDNNLIGTITLLEVMAAHGCKNLVFSSSATVYGWPKVVPCTEEFPL-EAMNPYGRTKLFIEEICRDVHRSDSEWKDHIAEIF 704
            MA+ +LVTGGAGYIGSHTVL+LL  GY  VV+DN  NA         ++ +V+EL G    ++ F ++D+ D+ AL+ LF K  F AVIHFAGL+AVGESVQKPL Y+  NL GTI LLE+M AHG KNLVFSSSATVYG P+ +P  E  P     NPYG++K FIEE+ RD+ ++D  W   +   F
Sbjct:    1 MAEKVLVTGGAGYIGSHTVLELLEAGYLPVVIDNFHNAFRGGGSLPESLRRVQELTGR---SVEFEEMDILDQGALQRLFKKHSFMAVIHFAGLKAVGESVQKPLDYYRVNLTGTIQLLEIMKAHGVKNLVFSSSATVYGNPQYLPLDEAHPTGGCTNPYGKSKFFIEEMIRDLCQADKTWNAVLLRYF 186

The following BLAST results are available for this feature:

BLAST of DR405962 vs. ExPASy Swiss-Prot

BLAST of DR405962 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 63


Match Name	E-value	Identity	Description
GALE3_ARATH	8.848e-78	75.43	UDP-glucose 4-epimerase 3 OS=Arabidopsis thaliana ...	[more]
GALE2_ARATH	1.154e-77	75.43	UDP-glucose 4-epimerase 2 OS=Arabidopsis thaliana ...	[more]
GALE2_CYATE	7.410e-77	77.01	UDP-glucose 4-epimerase GEPI48 OS=Cyamopsis tetrag...	[more]
GALE1_PEA	4.998e-57	60.00	UDP-glucose 4-epimerase OS=Pisum sativum GN=GALE P...	[more]
GALE1_ARATH	2.454e-56	58.76	UDP-glucose 4-epimerase 1 OS=Arabidopsis thaliana ...	[more]
GALE1_CYATE	8.254e-51	54.64	UDP-glucose 4-epimerase GEPI42 OS=Cyamopsis tetrag...	[more]
GALE_BACSU	1.076e-50	55.62	UDP-glucose 4-epimerase OS=Bacillus subtilis GN=ga...	[more]
GALE_DICDI	1.654e-48	52.78	UDP-glucose 4-epimerase OS=Dictyostelium discoideu...	[more]
GALE_MOUSE	8.799e-47	54.55	UDP-glucose 4-epimerase OS=Mus musculus GN=Gale PE...	[more]
GALE_PONAB	1.731e-46	53.97	UDP-glucose 4-epimerase OS=Pongo abelii GN=GALE PE...	[more]

Pages

Properties


Property Name	Value
Genbank description	CSAJ-PNP1248E24 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248E24 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR405962 ID=DR405962; Name=DR405962; organism=Citrus sinensis; type=EST; length=729bp
GGGGGTCAGTTTCATTTTTTTCTTTTCCTTTAAATTTCTCTGAGCGAGGC
CAAGCCCAAGTCTCATCACTTCCTTCGTAATTCCCCCGTTTCTTCAAATT
TTCGTGTACTTTGTGCCCTTTTTTTTGGTTCTTCTGATTGGTCTCTCCGT
TAGACTCCATGGCTAAGAACATTTTGGTGACCGGTGGCGCCGGTTACATT
GGAAGCCACACCGTGCTTCAGTTGCTGTTAGGCGGTTACAAAACTGTCGT
CGTCGACAATCTCGATAATGCCTCGGAAATCGCCGTCGCAAAAGTCAAAG
AACTCGCTGGCAATCAAGGCGACAACATGACCTTTCATAAGTTGGATCTC
CGGGACAAAGCAGCGCTGGAAGCATTGTTTGCCAAAACAAAGTTCGGTGC
TGTCATCCATTTTGCTGGACTGGAAGCAGTTGGTGAAAGTGTGCAGAAAC
CATTGCTGTATTTTGACAACAATCTTATTGGAACCATAACTCTCTTGGAA
GTTATGGCAGCACATGGCTGCAAAAATCTGGTCTTTTCATCATCAGCTAC
TGTTTATGGCTGGCCAAAGGTGGTTCCGTGTACAGAAGAGTTCCCGTTAG
AGGCAATGAACCCTTATGGACGTACCAAGCTTTTCATTGAAGAGATTTGC
CGTGATGTCCACCGCTCAGACTCTGAATGGAAAGATCATATTGCTGAGAT
ATTTCAATCCTGTTGNNNNNNATCCAAGT