CN189157

Overview
NameCN189157
Unique NameCN189157
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length779
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: TT12_ARATH (Protein TRANSPARENT TESTA 12 OS=Arabidopsis thaliana GN=TT12 PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 6.041e-40
Identity = 86/234 (36.75%), Postives = 138/234 (58.97%), Query Frame = 2
Query:   20 AILLLPLFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMKLNFGLPGALGSTILAYWIPNLGQLLFVICG-GCPQTWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLR 718
            A+ L  L++++ PIL  +GQ   IA      +  +IP ++AF ++   Q +LQAQ+    ++Y++     LH  L+WL T  L+FGL GA      ++W+      ++++    C +TW GFS  AF+ +WP  KL+++S  MLCLE+WYN  LV+++G + N  +++DA++IC+     +M   LG  AA+ VRVSNELG G+   A  ++ V   T++LI  VL +I L  R
Sbjct:  144 AVFLTFLYWYSGPILKTMGQSVAIAHEGQIFARGMIPQIYAFALACPMQRFLQAQNIVNPLAYMSLGVFLLHTLLTWLVTNVLDFGLLGAALILSFSWWLLVAVNGMYILMSPNCKETWTGFSTRAFRGIWPYFKLTVASAVMLCLEIWYNQGLVIISGLLSNPTISLDAISICMYYLNWDMQFMLGLSAAISVRVSNELGAGNPRVAMLSVVVVNITTVLISSVLCVIVLVFR 377          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: YJ2D_SCHPO (Uncharacterized transporter C4B3.13 OS=Schizosaccharomyces pombe GN=SPCC4B3.13 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.710e-18
Identity = 63/251 (25.10%), Postives = 117/251 (46.61%), Query Frame = 2
Query:    2 RVLTICAILLLPLFFF---TKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMK--LNFGLPGALGSTILAYWIPNLGQLLFVICGGCPQTWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            R+L I  ++  P+F      + IL+ L Q+      A     +++    A+ +    + +LQ Q     ++Y+ A  V +++FL++LF     + FG  GA  +  L  W      +++++     Q W GFS  A K+  P+ +L++    M+C E W   ++   +G +   E+A  ++++    + L   ++ G  AA   RV N +G G+   AK A  V++     IG ++ +I    R    YIF
Sbjct:  180 RILAILLLIQFPIFLIWWKIEGILLFLRQDPLTCMFAAKYMRVMMLASPAYALFEALKRFLQVQGIFHPVTYILAIVVPINIFLNYLFVWSPWVGFGFLGAPVAVALTLWSACAVLIIYIMKVNGRQAWGGFSREALKNWGPLCRLAVPGVIMICSEYWAFELVTFASGVLGTTELA--SMSVLSTTSTLSYNLAFGVAAAAATRVGNLIGAGNTKLAKLATHVSINLGAAIGVIIAVILFLTRNTWTYIF 428          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: S47A1_DANRE (Multidrug and toxin extrusion protein 1 OS=Danio rerio GN=slc47a1 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.502e-17
Identity = 66/248 (26.61%), Postives = 113/248 (45.56%), Query Frame = 2
Query:    5 VLTICAILLLPLFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMKLNFGLPGALGSTILAYWIPNLGQLLFVICGGC---PQTWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            +L +       L   T+PIL+A+ Q  N+A ++     + +P L A  +      YLQ Q          A    L+  ++++F   L  G+ G+  +  ++ +  +L   L+V          TW G+S    ++    ++L+L S  MLC+E W   +   L G +   E+   A ++   +  +  M  LGF  A  VRV N LG G+   AK + KVA+   +L+ CV+  +    +  +AYIF
Sbjct:  140 ILLLACFPCWALLINTEPILLAVRQSPNVASLSQLYVKIFMPALPAAFMYQLQGRYLQNQGIIWPQVITGAAGNILNALINYVFLHLLELGVAGSAAANTISQY--SLAVFLYVYIRWKNLHKATWDGWSRDCLQEWGAFIRLALPSMLMLCVEWWTYEIGGFLAGLISETELG--AQSVVYELATIAYMFPLGFAVAASVRVGNALGAGNTERAKLSAKVALVCGVLVSCVVATLIGCTKDVIAYIF 383          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: S47A1_RABIT (Multidrug and toxin extrusion protein 1 OS=Oryctolagus cuniculus GN=SLC47A1 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 3.566e-16
Identity = 66/246 (26.83%), Postives = 113/246 (45.93%), Query Frame = 2
Query:    5 VLTICAILLLPLFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMKLNFGLPG-ALGSTILAYWIPNLGQLLFVICGGCPQTWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            +L +C +    LF  T+ IL+   Q+  ++ +  T   + IP L A  +      YL  Q   +        +  ++   ++LF  +L+ G+ G AL +T+  + +  L  L  +       TW G+S    +D     +L++ S  MLC+E W   +   L+G +    V + A ++   +  +  MI +G   AV VRV N LG G+   AK +  VA+  + LI  V  ++ L  +  V YIF
Sbjct:  121 ILLLCCLPCWALFLNTQHILLLFRQDPAVSRLTQTYVTIFIPALPATFLYTLQVKYLLNQGIVLPQVVTGVAANLVNALANYLFVYQLHLGVMGSALANTVAQFTLALLLFLYILRSKVYQATWGGWSLECLQDWASFFRLAIPSMLMLCMEWWAYEIGSFLSGIL--GMVELGAQSVTYELAVIVYMIPMGLSVAVNVRVGNALGAGNIEQAKKSSAVALLVTELIAVVFCVMLLSCKDLVGYIF 364          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: YAO6_SCHPO (Uncharacterized transporter C11D3.06 OS=Schizosaccharomyces pombe GN=SPAC11D3.06 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.658e-16
Identity = 65/245 (26.53%), Postives = 111/245 (45.31%), Query Frame = 2
Query:   14 ICAILLLPL---FFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMKLNFGLPGALGSTILAYWIPNLGQLLFVICGGCPQTWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            + +IL LP+   ++++KPIL+ L Q   +AE +      LIP    ++     + +LQ Q      SY+   +  L++ L++L       GL GA  +T L+YW+  +    +       + W G++    ++  P VKLSL    M+  E W   ++ L+ G  K   V + A ++ +  + L   I  G       RV+  LG G  + A    KVA    + +G V+ I  + +R     IF
Sbjct:   95 VLSILYLPICLVWWYSKPILIFLHQTPELAEASQKFLRYLIPGGLGYVCFELLKKFLQTQEITRAGSYILLVTSPLNVALNFLLVHYYGLGLKGAPLATGLSYWLSFILLTQYAKYVKGAEAWNGWNKRCLENFGPFVKLSLLGIVMVGTEWWAFEIVALVAG--KLGAVPLAAQSVIMTTDQLLNTIPFGLGIITSNRVAYYLGAGLPDNASLTAKVAAIVGVAVGSVIMITMIAVRNIYGRIF 337          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: S47A1_HUMAN (Multidrug and toxin extrusion protein 1 OS=Homo sapiens GN=SLC47A1 PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.312e-15
Identity = 62/246 (25.20%), Postives = 113/246 (45.93%), Query Frame = 2
Query:    5 VLTICAILLLPLFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMKLNFGLPGALGSTILAYWIPNLGQLLFVICGGCPQ-TWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            VL +C      LF  T+ IL+   Q+ +++ +  T   + IP L A  +      YL  Q   +        +  ++   ++LF  +L+ G+ G+  + +++ +   L   L+++     Q TW G+S    +D    ++L++ S  MLC+E W   V   L+G +    V + A +I   +  +  M+  GF  A  VRV N LG G    A+ +  V++  ++L      ++ L  +  V YIF
Sbjct:  122 VLLLCCFPCWALFLNTQHILLLFRQDPDVSRLTQTYVTIFIPALPATFLYMLQVKYLLNQGIVLPQIVTGVAANLVNALANYLFLHQLHLGVIGSALANLISQYTLALLLFLYILGKKLHQATWGGWSLECLQDWASFLRLAIPSMLMLCMEWWAYEVGSFLSGIL--GMVELGAQSIVYELAIIVYMVPAGFSVAASVRVGNALGAGDMEQARKSSTVSLLITVLFAVAFSVLLLSCKDHVGYIF 365          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: S47A1_RAT (Multidrug and toxin extrusion protein 1 OS=Rattus norvegicus GN=Slc47a1 PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.498e-14
Identity = 64/248 (25.81%), Postives = 119/248 (47.98%), Query Frame = 2
Query:    5 VLTICAILLLPLFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNM--IISYLAACSVSLHLFLSWLFTMKLNFGLPGALGSTILAYWIPNLGQLLFVICGGCPQ-TWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            +L +C      LF  T+ IL+   Q+ +++ +  T  ++ IP L A  +      YL  Q   +  +I+ +AA  V+     ++LF  +L+ G+ G+  +  ++ +   +   L+++       TW G+S+   +D    ++L++ S  MLC+E W   V   L+G +    V + A +I   +  +  MI  GF  A  VRV N LG G+ + AK +  +++  + L      ++ L  +  V YIF
Sbjct:  121 ILLLCCFPCWALFINTEQILLLFRQDPDVSRLTQTYVMVFIPALPAAFLYTLQVKYLLNQGIVLPQVITGIAANLVNA--LANYLFLHQLHLGVMGSALANTISQFALAIFLFLYILWRKLHHATWGGWSWECLQDWASFLQLAIPSMLMLCIEWWAYEVGSFLSGIL--GMVELGAQSITYELAIIVYMIPAGFSVAANVRVGNALGAGNIDQAKKSSAISLIVTELFAVTFCVLLLGCKDLVGYIF 364          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: S47A1_MOUSE (Multidrug and toxin extrusion protein 1 OS=Mus musculus GN=Slc47a1 PE=1 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 1.498e-14
Identity = 60/247 (24.29%), Postives = 118/247 (47.77%), Query Frame = 2
Query:    5 VLTICAILLLPLFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSL-HLFLSWLFTMKLNFGLPGALGSTILAYWIPNLGQLLFVICGGCPQ-TWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            +L +C      LF  T+ IL+   Q+ +++ +  T  ++ IP L A  + +T Q+        ++   +   + +L +   +++F   L+ G+ G+  +  ++ +   +   L+++     Q TW G+S+   +D    ++L++ S  MLC+E W   V   L+G +    V + A +I   +  +  MI  GF  A  VRV N LG G+ + AK +  +++  + L      ++ L  +  V YIF
Sbjct:  122 ILLLCCFPCWALFINTEQILLLFRQDPDVSRLTQTYVMIFIPALPAAFL-YTLQVKYLLNQGIVLPQIMTGIAANLVNALANYVFLYHLHLGVMGSALANTISQFALAIFLFLYILWRRLHQATWGGWSWECLQDWASFLRLAIPSMLMLCIEWWAYEVGSFLSGIL--GMVELGAQSITYELAIIVYMIPSGFSVAANVRVGNALGAGNIDQAKKSSAISLIVTELFAVTFCVLLLGCKDLVGYIF 365          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: S47A1_PONAB (Multidrug and toxin extrusion protein 1 OS=Pongo abelii GN=SLC47A1 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.957e-14
Identity = 67/248 (27.02%), Postives = 110/248 (44.35%), Query Frame = 2
Query:    5 VLTICAILLLPLFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMKLNFGLPG-ALGSTILAYWIPNLGQLLFVICGGC--PQTWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLIGCVLFIIFLFLRGRVAYIF 739
            +L +C      LF  T+ IL+   Q+ +++ +  T   + IP L A  +      YL  Q   +        +  ++   ++LF  +L+ G  G AL + I  Y +  L  L F I G      TW G+S    +D    + L++ S  MLC+E W   V   L+G +    V + A +I   +  +  M+  GF  A  VRV N LG G    A+ +  V++  ++L      ++ L  +  V YIF
Sbjct:  122 ILLLCCFPCWALFLNTQHILLLFRQDPDVSRLTQTYVTIFIPALPATFLYMLQVKYLLNQGIVLPQIVTGVAANLVNALANYLFLHQLHLGAIGSALANLISQYTLALL--LFFYILGKKLHQATWGGWSLECLQDWASFLHLAVPSMLMLCMEWWAYEVGSFLSGIL--GMVELGAQSIVYELAIIVYMVPAGFSVAASVRVGNALGAGDMEQARKSSTVSLLITVLFAVAFSVLLLSCKDHVGYIF 365          
BLAST of CN189157 vs. ExPASy Swiss-Prot
Match: YL47_SCHPO (Uncharacterized transporter C323.07c OS=Schizosaccharomyces pombe GN=SPAC323.07c PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.360e-14
Identity = 61/232 (26.29%), Postives = 104/232 (44.83%), Query Frame = 2
Query:    2 RVLTICAILLLP---LFFFTKPILVALGQEENIAEVAGTISLMLIPVLFAFIISFTCQMYLQAQSRNMIISYLAACSVSLHLFLSWLFTMK--LNFGLPGALGSTILAYWIPNLGQLLFVICGGCPQTWKGFSFAAFKDLWPVVKLSLSSGAMLCLELWYNTVLVLLTGNMKNAEVAIDALAICININGLEMMISLGFLAAVGVRVSNELGRGSANAAKFAIKVAVFTSLLI 682
            R L I A+L +P   ++   + IL+ L Q+  +A + G    + I     + +    + YLQAQ     I+Y+   +  L++ L++L      + FG  GA  +    +W  ++  +L++     P  W GFS  A K+L P++  S     M+  E     +  L  G +  A +A  + +I +    L   I   F  A   RV + +G G AN A+   +VA   +L I
Sbjct:  174 RCLLILAVLHIPVALIWLNLEHILIFLHQDPMVAHLCGRYMRVFILAAPGYAVFEALKRYLQAQGIFTPITYVLCFAAPLNILLNYLLVWHPTIGFGFLGAPVAVATTFWFQSICLILYICFSSTPIPWPGFSRQALKNLSPMLHFSFHGMLMIVTEWAAYEMTSLGAGYLGTAPLA--SQSILLTSTSLLFQIPFAFAVASSTRVGHLIGSGRANLARLCSRVAYSLALCI 403          
The following BLAST results are available for this feature:
BLAST of CN189157 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
TT12_ARATH6.041e-4036.75Protein TRANSPARENT TESTA 12 OS=Arabidopsis thalia... [more]
YJ2D_SCHPO1.710e-1825.10Uncharacterized transporter C4B3.13 OS=Schizosacch... [more]
S47A1_DANRE5.502e-1726.61Multidrug and toxin extrusion protein 1 OS=Danio r... [more]
S47A1_RABIT3.566e-1626.83Multidrug and toxin extrusion protein 1 OS=Oryctol... [more]
YAO6_SCHPO4.658e-1626.53Uncharacterized transporter C11D3.06 OS=Schizosacc... [more]
S47A1_HUMAN2.312e-1525.20Multidrug and toxin extrusion protein 1 OS=Homo sa... [more]
S47A1_RAT1.498e-1425.81Multidrug and toxin extrusion protein 1 OS=Rattus ... [more]
S47A1_MOUSE1.498e-1424.29Multidrug and toxin extrusion protein 1 OS=Mus mus... [more]
S47A1_PONAB1.957e-1427.02Multidrug and toxin extrusion protein 1 OS=Pongo a... [more]
YL47_SCHPO4.360e-1426.29Uncharacterized transporter C323.07c OS=Schizosacc... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001G14_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001G14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189157 ID=CN189157; Name=CN189157; organism=Citrus sinensis; type=EST; length=779bp
GAGGGTCTTAACCATTTGTGCTATCCTTCTCTTACCTCTGTTCTTCTTCA
CTAAACCAATTTTGGTGGCTTTGGGCCAAGAAGAAAATATTGCTGAAGTG
GCGGGAACTATTTCTCTCATGCTAATCCCTGTGTTGTTCGCCTTCATCAT
ATCCTTTACCTGCCAAATGTACCTACAAGCCCAGAGCAGAAACATGATTA
TCTCCTACTTGGCGGCATGTTCAGTTTCGCTTCATCTCTTTCTTTCGTGG
CTTTTTACCATGAAATTGAATTTTGGACTCCCTGGAGCATTGGGATCAAC
AATTCTGGCATACTGGATTCCAAACTTGGGTCAACTTCTGTTTGTTATAT
GTGGAGGGTGCCCTCAAACATGGAAGGGTTTCTCATTTGCTGCTTTCAAG
GATCTCTGGCCTGTAGTAAAACTTTCCTTGTCGTCTGGCGCCATGCTATG
TCTCGAGCTCTGGTACAACACAGTCTTGGTTCTTTTAACAGGAAACATGA
AAAATGCTGAGGTTGCCATTGATGCTCTTGCAATATGCATCAACATCAAT
GGGCTAGAAATGATGATCTCGCTTGGTTTCTTGGCTGCAGTAGGTGTTAG
GGTGTCAAATGAGCTTGGCCGAGGAAGCGCAAATGCTGCAAAATTTGCTA
TTAAGGTGGCAGTTTTCACATCGCTTTTGATTGGATGTGTGCTGTTTATC
ATTTTCCTCTTCCTCAGAGGACGCGTAGCATACATTTTTCCCCGACAATC
AAGATGTGATTGACACAGTTGCAGATCTA
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