EY666148

Overview
NameEY666148
Unique NameEY666148
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length855
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.303e-21
Identity = 62/204 (30.39%), Postives = 99/204 (48.53%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEALELKEKG-TTILGVELKFKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKYRSKDF 618
            YGP+  L +G    VV +S S+  Q ++  D  F+ R P        +   D+ ++ YGP WR LRK     + S  +LD    +R+ EVK   R L +      KP+ +G+L       A+  ++ G+ +     G       E K  + E+MV+ G  NI D  P L+WLDIQG+  + KK+   F+  +   +E+++ K F
Sbjct:   64 YGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQ---KPVKLGQLLNVCTTNALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKLHARFDAFLTDILEEHKGKIF 264          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 8.973e-19
Identity = 61/214 (28.50%), Postives = 107/214 (50.00%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSI--STLVCAMQNMLWGEALELK----EKGTTILGVELKFKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKYRSKDFNVEGK 633
            YGPI  L +G    VV S+P+  K  ++  D  F+ R P        +   D+ ++ YGP W+ LRK     M+   +L+    +R  E+ + ++ +++ ++       +GE  +    L  AM NM+ G+ +  K    EKG  +   E K  + ELM + G  NI D  P ++W+D+QGIE+  KK+   F++++    E++ +     +GK
Sbjct:   67 YGPIMYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRPPNAGATHMAYNAQDMVFAPYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASH-------VGERIVVADMLTFAMANMI-GQVMLSKRVFVEKGKEV--NEFKNMVVELMTVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHKKFDDLLTKMFEEHEATSNERKGK 270          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C75A6_CAMME (Flavonoid 3',5'-hydroxylase OS=Campanula medium GN=CYP75A6 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.453e-18
Identity = 58/209 (27.75%), Postives = 105/209 (50.24%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNK-ICKPIDIGELSISTLVCAMQNMLWGEAL---------ELKEKGTTILGV-ELKFKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKY 603
            YGPI  L LG+K  VV S+P   +  ++  D  F+ R          +   D+ ++ YGP+W+ LRK     M+   +L+    ++  EV + ++++Y  ++K +  P+ + E+    L  AM NM+    L         +L    +    V E ++ + ELM + G  NI D  P ++W+D+QGI+R  K I   F+ ++N  ++++
Sbjct:   69 YGPIMYLKLGSKGTVVASNPKAARAFLKTHDANFSNRPIDGGPTYLAYNAQDMVFAEYGPKWKLLRKLCSLHMLGPKALEDWAHVKVSEVGHMLKEMYEQSSKSVPVPVVVPEM----LTYAMANMIGRIILSRRPFVITSKLDSSASASASVSEFQYMVMELMRMAGLFNIGDFIPYIAWMDLQGIQRDMKVIQKKFDVLLNKMIKEH 273          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C75A1_PINTA (Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.816e-18
Identity = 60/201 (29.85%), Postives = 104/201 (51.74%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEALELKEKGTTILGVELK---FKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKY 603
            YGPI  L  G+   VVVSS    K  ++  D  FA R P   G    +   DIA+S YG  WRK+RK  V +++++  +++   +R++E+   I  ++  +      +++ + +IST   ++ N+LW   L  K+     LG + K     + E+ + +G+ NI D  P L  LD+QGI+R  KK +  F+      ++++
Sbjct:   73 YGPILFLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSK-AIST---SLANILW-RILARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFDAFAEKMIDEH 268          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 7.596e-18
Identity = 58/212 (27.36%), Postives = 107/212 (50.47%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEALELK----EKGTTILGVELKFKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKYRSKDFNVEGK 633
            YG I  L +G    VV S+P   K  ++  D  F+ R P        +G  D+ +++YGP W+ LRK     M+   +L+    +R  E+ + ++ +++ + +  + + + E+    L  AM NM+ G+ +  K     KG  +   E K  + ELM   G  NI D  P L+W+D+QGIE+  K++   F+ ++    +++++  +  +GK
Sbjct:   64 YGAIMYLKVGTCGMVVASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYGAQDMVFAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDMSRE-GERVVVAEM----LTFAMANMI-GQVILSKRVFVNKGVEV--NEFKDMVVELMTTAGYFNIGDFIPCLAWMDLQGIEKGMKRLHKKFDALLTKMFDEHKATSYERKGK 267          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.692e-17
Identity = 56/211 (26.54%), Postives = 106/211 (50.24%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEALELK---EKGTTILGVELKFKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKYRSKDFNVEGK 633
            YG I  L +G     V S+P   K  ++  D  F+ R P        +   D+ +++YGP W+ LRK     M+   +L+    +R  E+ + ++ + ++ ++  + + + E+    L  AM NM+    L  +   +KG  +   E K  + ELM + G  NI D  P L+W+D+QGIE+R K++   F+ ++    +++++  +  +GK
Sbjct:   64 YGAIMYLKVGTCGMAVASTPDAAKAFLKTLDINFSNRPPNAGATHLAYNAQDMVFAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSM-SDMSREGQRVVVAEM----LTFAMANMIGQVMLSKRVFVDKGVEVN--EFKDMVVELMTIAGYFNIGDFIPCLAWMDLQGIEKRMKRLHKKFDALLTKMFDEHKATTYERKGK 267          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.886e-17
Identity = 59/201 (29.35%), Postives = 98/201 (48.76%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEAL---ELKEKGTTILGVELKFKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKY 603
            YGPI  L LG    VV +S S+ +Q ++  D  FA R P        +   D+ ++ YG  WR LRK     + S  +L+    +R++EV    R+L        KP+++G+L    +V A+   + G  L   +   K       E +  + E+M L G  NI D  P L WLD+QG+  + K++   F+  ++S ++++
Sbjct:   64 YGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGT---KPVNLGQLVNMCVVNALGREMIGRRLFGADADHKAD-----EFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLSSILKEH 256          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C71DB_LOTJA (Cytochrome P450 71D11 (Fragment) OS=Lotus japonicus GN=CYP71D11 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 8.398e-17
Identity = 59/214 (27.57%), Postives = 103/214 (48.13%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEALELKEKGTTILGVELKFKLAELMVLLGTPNISDIFPMLSWLD-----IQGIERRAKKISLWFENIINSTVEKYRSKDFNVEGKE 636
            YGP+  L LG  + ++VSS    K+V++  D TFA R   +   +  +G  DI +S YG  WR++RK    +++S   + + + +R++EVKN I+ + +    +   +++ +   S +        +G+    +E        E    + E+M L G  NI+D+FP   WL+         E   +K+    E II+      R+K+  VEG E
Sbjct:   51 YGPLMHLQLGEVIFIIVSSAEYAKEVMKTHDVTFASRPRSLFTDIVFYGSTDIGFSPYGDYWRQVRKICNVELLSMKRVQSLWPIREEEVKNLIQRIASEEGSV---VNLSQAIDSLIFTITSRSAFGKRYMEQE--------EFISCVREVMKLAGGFNIADLFPSAKWLENLTRMRSKFEYLHQKMDRILETIIDDHKANSRTKEGQVEGGE 253          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 87.0409 bits (214), Expect = 1.433e-16
Identity = 53/203 (26.11%), Postives = 102/203 (50.25%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEALELKEKGTTILGVELKFK--LAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKYRSK 612
            YG +F LW G++L VVVSS S  +Q     D   A R   ++G    +    +  ++YG  WR LR+     ++SN  +++   +R+ E +  I  L ++++     +++           +  M+ G+    ++  T+ L    +F+  ++EL+ L G  N +D  P+L +LD + +E+R K IS   +  +   +E++R+K
Sbjct:   63 YGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTK 265          
BLAST of EY666148 vs. ExPASy Swiss-Prot
Match: C84A1_ARATH (Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CYP84A1 PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.871e-16
Identity = 62/204 (30.39%), Postives = 99/204 (48.53%), Query Frame = 1
Query:   10 YGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFAYRDPPIAGLVATFGGNDIAWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKQEVKNTIRDLYNNNNKICKPIDIGELSISTLVCAMQNMLWGEALELKEKGTTILGVELKFKLAELMVLLGTPNISDIFPMLSWLDIQGIERRAKKISLWFENIINSTVEKYRSKDFN 621
            YG +  L +G      VSSP + +QV++ QD+ F+ R   IA    T+   D+A+++YGP WR++RK  V K+ S    ++ +A  + EV   +R +  N   + KPI++GE   +          +G A    EKG      E    L E   L G  N++D  P   W+D QGI +R  K     +  I+  ++++  K  N
Sbjct:   71 YGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRKVCVMKVFSRKRAES-WASVRDEVDKMVRSVSCN---VGKPINVGEQIFALTRNITYRAAFGSAC---EKGQD----EFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDDIIDEHMKKKEN 263          
The following BLAST results are available for this feature:
BLAST of EY666148 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 46
Match NameE-valueIdentityDescription
F3PH_PETHY4.303e-2130.39Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=C... [more]
C75A2_SOLME8.973e-1928.50Flavonoid 3',5'-hydroxylase OS=Solanum melongena G... [more]
C75A6_CAMME4.453e-1827.75Flavonoid 3',5'-hydroxylase OS=Campanula medium GN... [more]
C75A1_PINTA5.816e-1829.85Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 P... [more]
C75A3_PETHY7.596e-1827.36Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida G... [more]
C75A1_PETHY1.692e-1726.54Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida G... [more]
F3PH_ARATH2.886e-1729.35Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana... [more]
C71DB_LOTJA8.398e-1727.57Cytochrome P450 71D11 (Fragment) OS=Lotus japonicu... [more]
C81E1_GLYEC1.433e-1626.11Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ... [more]
C84A1_ARATH1.871e-1630.39Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CY... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-102-014-F03-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY666148 ID=EY666148; Name=EY666148; organism=Citrus sinensis; type=EST; length=855bp
TCCGGAGTTTATGGTCCGATCTTCAAGCTCTGGCTCGGAAATAAATTATG
TGTAGTGGTGAGCTCACCTTCCCTAGTTAAACAAGTGGTTCGTGATCAAG
ATACAACATTCGCATACCGTGACCCGCCGATCGCTGGACTAGTGGCCACC
TTTGGTGGCAATGATATTGCATGGTCCAATTACGGTCCCGAATGGAGAAA
GTTGCGCAAATTTTTTGTGGGCAAGATGATGAGTAACGCAAGCCTTGATG
CTTGTTATGCTCTAAGAAAACAAGAGGTTAAAAATACTATCAGGGATTTG
TATAACAACAACAACAAAATTTGCAAGCCCATTGACATTGGTGAATTGTC
GATTTCAACACTGGTATGTGCTATGCAGAACATGCTGTGGGGTGAAGCGC
TTGAATTAAAAGAGAAAGGGACCACTATTCTTGGGGTTGAGCTAAAATTT
AAGCTTGCAGAACTCATGGTGCTACTGGGAACACCGAATATTTCGGATAT
CTTTCCAATGCTTTCATGGCTCGACATACAAGGTATAGAGAGGAGAGCAA
AGAAGATTTCACTCTGGTTTGAAAATATTATAAATTCTACTGTTGAAAAG
TATAGAAGCAAGGACTTTAATGTTGAAGGTAAAGAAAGAACAAGGGGAAA
ATTTTGAAGGGATCAGGGAACAGAAACTTCTTGCAGTTACTATTGGAACT
TTCAAAAAAAAGGAAAAACGGTCTTCCCTCATTTAACATGACCAATTTTA
AGGGCGGGGTTCGCGGTATTGTATACGACATAGCCCGAAAAGATTGTAAA
TTTGGACGGAGGGCGCCCTTTTCCAATTTTTTTTAATATTTTGAAAAAAC
AAACC
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