CN181596

Overview
NameCN181596
Unique NameCN181596
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length555
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL35_EUPES (60S ribosomal protein L35 OS=Euphorbia esula GN=RPL35 PE=2 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 3.930e-44
Identity = 88/99 (88.89%), Postives = 91/99 (91.92%), Query Frame = -1
Query:  217 RPSFALLRV---TGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIKV 504
            +   ALLRV   TGGAPNKLSKIKVVRLS+AQVLTVISQKQK ALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQ SLKTEREKK+EMYFPMRKYAIKV
Sbjct:   25 KAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKLALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQQSLKTEREKKKEMYFPMRKYAIKV 123          

HSP 2 Score: 31.187 bits (69), Expect = 3.930e-44
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = -2
Query:  501 KSKVDLLNQLKELKAELA 554
            K+K +LLNQLK+LKAELA
Sbjct:   12 KTKAELLNQLKDLKAELA 29          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL354_ARATH (60S ribosomal protein L35-4 OS=Arabidopsis thaliana GN=RPL35D PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 4.260e-43
Identity = 86/99 (86.87%), Postives = 91/99 (91.92%), Query Frame = -1
Query:  217 RPSFALLRV---TGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIKV 504
            +   ALLRV   TGGAPNKLSKIKVVR S+AQVLTVISQKQK+ALREAYKNKK LPLDLRPKKTRAIRRRLTKHQASLKTEREKK+EMYFP+RKYAIKV
Sbjct:   25 KAELALLRVAKVTGGAPNKLSKIKVVRKSIAQVLTVISQKQKSALREAYKNKKLLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKEMYFPVRKYAIKV 123          

HSP 2 Score: 31.187 bits (69), Expect = 4.260e-43
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = -2
Query:  501 KSKVDLLNQLKELKAELA 554
            KSK DL NQLKE KAELA
Sbjct:   12 KSKTDLQNQLKEFKAELA 29          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL351_ARATH (60S ribosomal protein L35-1 OS=Arabidopsis thaliana GN=RPL35A PE=1 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 1.228e-42
Identity = 85/99 (85.86%), Postives = 90/99 (90.91%), Query Frame = -1
Query:  217 RPSFALLRV---TGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIKV 504
            +   ALLRV   TGGAPNKLSKIKVVR S+AQVLTV SQKQK+ALREAYKNKK LPLDLRPKKTRAIRRRLTKHQASLKTEREKK+EMYFP+RKYAIKV
Sbjct:   25 KAELALLRVAKVTGGAPNKLSKIKVVRKSIAQVLTVSSQKQKSALREAYKNKKLLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKEMYFPIRKYAIKV 123          

HSP 2 Score: 31.9574 bits (71), Expect = 1.228e-42
Identity = 16/18 (88.89%), Postives = 16/18 (88.89%), Query Frame = -2
Query:  501 KSKVDLLNQLKELKAELA 554
            KSK DL NQLKELKAELA
Sbjct:   12 KSKSDLQNQLKELKAELA 29          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL352_ARATH (60S ribosomal protein L35-2 OS=Arabidopsis thaliana GN=RPL35B PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 2.086e-42
Identity = 86/99 (86.87%), Postives = 91/99 (91.92%), Query Frame = -1
Query:  217 RPSFALLRV---TGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIKV 504
            +   ALLRV   TGGAPNKLSKIKVVR S+AQVLTVISQKQK+ALREAYKNKK LPLDLRPKKTRAIRRRLTKHQASLKTEREKK+EMYFP+RKYAIKV
Sbjct:   25 KAELALLRVAKVTGGAPNKLSKIKVVRKSIAQVLTVISQKQKSALREAYKNKKLLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKEMYFPIRKYAIKV 123          

HSP 2 Score: 28.8758 bits (63), Expect = 2.086e-42
Identity = 14/18 (77.78%), Postives = 14/18 (77.78%), Query Frame = -2
Query:  501 KSKVDLLNQLKELKAELA 554
            KSK DL  QLKE KAELA
Sbjct:   12 KSKADLSGQLKEFKAELA 29          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL353_ARATH (60S ribosomal protein L35-3 OS=Arabidopsis thaliana GN=RPL35C PE=1 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.021e-41
Identity = 81/94 (86.17%), Postives = 88/94 (93.62%), Query Frame = -1
Query:  217 SFALLRVTGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIKV 498
            S  + +VTGGAPNKLSKIKVVR S+AQVLTV SQKQK+ALREAYKNKK LPLDLRPKKTRAIRRRLTKHQASLKTEREKK++MYFP+RKYAIKV
Sbjct:   30 SLRVAKVTGGAPNKLSKIKVVRKSIAQVLTVSSQKQKSALREAYKNKKLLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKDMYFPIRKYAIKV 123          

HSP 2 Score: 29.6462 bits (65), Expect = 1.021e-41
Identity = 15/18 (83.33%), Postives = 15/18 (83.33%), Query Frame = -2
Query:  501 KSKVDLLNQLKELKAELA 554
            KSK DL  QLKELKAELA
Sbjct:   12 KSKSDLSTQLKELKAELA 29          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL35_RAT (60S ribosomal protein L35 OS=Rattus norvegicus GN=Rpl35 PE=1 SV=3)

HSP 1 Score: 120.553 bits (301), Expect = 5.321e-27
Identity = 57/88 (64.77%), Postives = 76/88 (86.36%), Query Frame = -1
Query:  220 RVTGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIK 483
            +VTGGA +KLSKI+VVR S+A+VLTVI+Q QK  LR+ YK KK+ PLDLRPKKTRA+RRRLTKH+  LKT++++++E  +P+RKYA+K
Sbjct:   35 KVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLTKHEEKLKTKKQQRKERLYPLRKYAVK 122          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL35_MOUSE (60S ribosomal protein L35 OS=Mus musculus GN=Rpl35 PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.321e-27
Identity = 57/88 (64.77%), Postives = 76/88 (86.36%), Query Frame = -1
Query:  220 RVTGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIK 483
            +VTGGA +KLSKI+VVR S+A+VLTVI+Q QK  LR+ YK KK+ PLDLRPKKTRA+RRRLTKH+  LKT++++++E  +P+RKYA+K
Sbjct:   35 KVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLTKHEEKLKTKKQQRKERLYPLRKYAVK 122          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL35_HUMAN (60S ribosomal protein L35 OS=Homo sapiens GN=RPL35 PE=1 SV=2)

HSP 1 Score: 119.013 bits (297), Expect = 1.548e-26
Identity = 56/88 (63.64%), Postives = 76/88 (86.36%), Query Frame = -1
Query:  220 RVTGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIK 483
            +VTGGA +KLSKI+VVR S+A+VLTVI+Q QK  LR+ YK KK+ PLDLRPKKTRA+RRRL KH+ +LKT++++++E  +P+RKYA+K
Sbjct:   35 KVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVK 122          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL35_CHICK (60S ribosomal protein L35 OS=Gallus gallus GN=RPL35 PE=2 SV=3)

HSP 1 Score: 119.013 bits (297), Expect = 1.548e-26
Identity = 57/88 (64.77%), Postives = 76/88 (86.36%), Query Frame = -1
Query:  220 RVTGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIK 483
            +VTGGA +KLSKI+VVR S+A+VLTVI+Q QK  LR+ YK KK+ PLDLRPKKTRA+RRRL KH+ +LKT++++++E  +P+RKYAIK
Sbjct:   35 KVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPVRKYAIK 122          
BLAST of CN181596 vs. ExPASy Swiss-Prot
Match: RL35_BOVIN (60S ribosomal protein L35 OS=Bos taurus GN=RPL35 PE=2 SV=3)

HSP 1 Score: 119.013 bits (297), Expect = 1.548e-26
Identity = 56/88 (63.64%), Postives = 76/88 (86.36%), Query Frame = -1
Query:  220 RVTGGAPNKLSKIKVVRLSLAQVLTVISQKQKAALREAYKNKKFLPLDLRPKKTRAIRRRLTKHQASLKTEREKKREMYFPMRKYAIK 483
            +VTGGA +KLSKI+VVR S+A+VLTVI+Q QK  LR+ YK KK+ PLDLRPKKTRA+RRRL KH+ +LKT++++++E  +P+RKYA+K
Sbjct:   35 KVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYAVK 122          
The following BLAST results are available for this feature:
BLAST of CN181596 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 26
Match NameE-valueIdentityDescription
RL35_EUPES3.930e-4488.8960S ribosomal protein L35 OS=Euphorbia esula GN=RP... [more]
RL354_ARATH4.260e-4386.8760S ribosomal protein L35-4 OS=Arabidopsis thalian... [more]
RL351_ARATH1.228e-4285.8660S ribosomal protein L35-1 OS=Arabidopsis thalian... [more]
RL352_ARATH2.086e-4286.8760S ribosomal protein L35-2 OS=Arabidopsis thalian... [more]
RL353_ARATH1.021e-4186.1760S ribosomal protein L35-3 OS=Arabidopsis thalian... [more]
RL35_RAT5.321e-2764.7760S ribosomal protein L35 OS=Rattus norvegicus GN=... [more]
RL35_MOUSE5.321e-2764.7760S ribosomal protein L35 OS=Mus musculus GN=Rpl35... [more]
RL35_HUMAN1.548e-2663.6460S ribosomal protein L35 OS=Homo sapiens GN=RPL35... [more]
RL35_CHICK1.548e-2664.7760S ribosomal protein L35 OS=Gallus gallus GN=RPL3... [more]
RL35_BOVIN1.548e-2663.6460S ribosomal protein L35 OS=Bos taurus GN=RPL35 P... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001D02_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001D02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181596 ID=CN181596; Name=CN181596; organism=Citrus sinensis; type=EST; length=555bp
CCCCTTTTTTTTTTTTTTTGACAGAAATCCTATCTTGACTGCTATTCCAT
CACAACCTTTCACATCTTGTTTAGGGTGAGATGCACCCTTCAAGTTTTAC
ACACATAAGATTGAACAAAAATGATATTTGAAAAGGTAACAAAGTAAAAA
TTTGTCAACAAAATAGGTTCGTCACTGCAAGATACCAAGATACTCCTAGA
AAAAGATTCTAGCCTACACCTTAATAGCGTACTTCCTCATGGGAAAGTAC
ATCTCCCTCTTCTTCTCCCGCTCGGTCTTCAGTGAAGCCTGGTGCTTGGT
AAGCCTTCTCCTGATAGCCCGGGTCTTCTTGGGACGCAGATCAAGGGGCA
AGAACTTCTTATTCTTGTATGCCTCCCTCAATGCAGCCTTCTGCTTTTGT
GAAATGACAGTCAACACTTGTGCGAGCGACAACCTCACCACCTTGATCTT
GGATAGCTTGTTAGGGGCACCGCCGGTGACCCTTAGCAACGCGAAGGAGG
GGCGAGCTCAGCCTTGAGTTCCTTCAACTGGTTCAAAAGATCCACCTTCG
ATTTC
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