CN188428

Overview
NameCN188428
Unique NameCN188428
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length461
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_MEDSA (Malate dehydrogenase, cytoplasmic OS=Medicago sativa GN=CMDH PE=2 SV=1)

HSP 1 Score: 250.751 bits (639), Expect = 2.117e-66
Identity = 123/153 (80.39%), Postives = 132/153 (86.27%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LILKEFAPSIP +  + LTRLDHNRALGQISE+LNVQVSDVKNVIIWGNHSS+QYPDVNHATVNT AGEKPVR+LV DDAWLNGEFI+TVQQRGAAIIKARK           CDHIRDWVLGTP+GT+VSMGVYSDGSYNVP+GLI
Sbjct:  133 PANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVNTPAGEKPVRQLVSDDAWLNGEFISTVQQRGAAIIKARKLSSALSAASAACDHIRDWVLGTPQGTFVSMGVYSDGSYNVPSGLI 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_MAIZE (Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2 SV=2)

HSP 1 Score: 250.751 bits (639), Expect = 2.117e-66
Identity = 125/153 (81.70%), Postives = 129/153 (84.31%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LILKEFAPSIP K  T LTRLDHNRALGQISE+LNVQVSDVKNVIIWGNHSSSQYPDVNHATV T+ GEKPVRELV DD WLNGEFITTVQQRGAAIIKARK           CDHIRDWVLGTPEGT+VSMGVYSDGSY VP+GLI
Sbjct:  133 PANTNALILKEFAPSIPEKNVTCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSSQYPDVNHATVKTSTGEKPVRELVSDDEWLNGEFITTVQQRGAAIIKARKFSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPSGLI 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_MESCR (Malate dehydrogenase, cytoplasmic OS=Mesembryanthemum crystallinum GN=MDH PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 6.159e-66
Identity = 123/153 (80.39%), Postives = 128/153 (83.66%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LILKEFAPSIP K  + LTRLDHNRALGQISE+LNVQVSDVKNVIIWGNHSS+QYPDVNHATV T   +KPVRELV DDAWLNGEFITTVQQRGAAIIKARK           CDHI DWVLGTPEGTWVSMGVYSDGSYNVPAG+I
Sbjct:  133 PANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVKTQGVDKPVRELVADDAWLNGEFITTVQQRGAAIIKARKLSSALSAASSACDHIHDWVLGTPEGTWVSMGVYSDGSYNVPAGII 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_BETVU (Malate dehydrogenase, cytoplasmic OS=Beta vulgaris GN=NR1 PE=1 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 6.159e-66
Identity = 124/153 (81.05%), Postives = 127/153 (83.01%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LILKEFAPSIP K  T LTRLDHNRALGQISE+LN QVSDVKNVIIWGNHSSSQYPDVN  TV T +GEK VRELV DDAWLNGEFITTVQQRGAAIIKARK           CDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAG+I
Sbjct:  133 PANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISERLNAQVSDVKNVIIWGNHSSSQYPDVNPCTVKTGSGEKAVRELVADDAWLNGEFITTVQQRGAAIIKARKLSSALSAASSACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGII 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC2_ARATH (Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis thaliana GN=At5g43330 PE=2 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 5.214e-65
Identity = 121/153 (79.08%), Postives = 130/153 (84.97%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LILKEFAPSIP K  T LTRLDHNRALGQ+SE+L+V VSDVKNVIIWGNHSS+QYPDVNHATV T+ GEKPVRELVK+D WLNGEFI+TVQQRGAAIIKARK           CDHIRDWV+GTPEGT+VSMGVYSDGSYNVPAGLI
Sbjct:  133 PANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVKNVIIWGNHSSTQYPDVNHATVKTSVGEKPVRELVKNDEWLNGEFISTVQQRGAAIIKARKLSSALSAASSACDHIRDWVVGTPEGTFVSMGVYSDGSYNVPAGLI 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_ORYSJ (Malate dehydrogenase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os10g0478200 PE=1 SV=3)

HSP 1 Score: 244.202 bits (622), Expect = 1.981e-64
Identity = 122/153 (79.74%), Postives = 127/153 (83.01%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LILKEFAPSIP K  T LTRLDHNRALGQISEKLNVQV+DVKN IIWGNHSS+QYPDVNHATV T +GEKPVRELV DD WLN EFI+TVQQRGAAIIKARK           CDHIRDWVLGTPEGT+VSMGVYSDGSY VPAGLI
Sbjct:  133 PANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVTDVKNAIIWGNHSSTQYPDVNHATVKTPSGEKPVRELVADDEWLNTEFISTVQQRGAAIIKARKQSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPAGLI 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC1_ARATH (Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis thaliana GN=At1g04410 PE=1 SV=2)

HSP 1 Score: 244.202 bits (622), Expect = 1.981e-64
Identity = 121/153 (79.08%), Postives = 131/153 (85.62%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLI 460
            P   N LILKEFAPSIP K  + LTRLDHNRALGQISE+L+V VSDVKNVIIWGNHSSSQYPDVNHA V T++GEKPVRELVKDDAWL+GEFI+TVQQRGAAIIKARK           CDHIRDWVLGTPEGT+VSMGVYSDGSY+VP+GLI
Sbjct:  133 PANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVKNVIIWGNHSSSQYPDVNHAKVQTSSGEKPVRELVKDDAWLDGEFISTVQQRGAAIIKARKLSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYSVPSGLI 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_PIG (Malate dehydrogenase, cytoplasmic OS=Sus scrofa GN=MDH1 PE=1 SV=4)

HSP 1 Score: 179.874 bits (455), Expect = 4.589e-45
Identity = 95/154 (61.69%), Postives = 106/154 (68.83%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDG-SYNVPAGLI 460
            P   N L   + APSIP + F+ LTRLDHNRA  QI+ KL V   DVKNVIIWGNHSS+QYPDVNHA V   A E  V E VKDD+WL GEFITTVQQRGAA+IKARK           CDH+RD   GTPEG +VSMG+ SDG SY VP  L+
Sbjct:  132 PANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVKLQAKEVGVYEAVKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGIISDGNSYGVPDDLL 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_HUMAN (Malate dehydrogenase, cytoplasmic OS=Homo sapiens GN=MDH1 PE=1 SV=4)

HSP 1 Score: 178.333 bits (451), Expect = 1.335e-44
Identity = 93/154 (60.39%), Postives = 106/154 (68.83%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDG-SYNVPAGLI 460
            P   N L   + APSIP + F+ LTRLDHNRA  QI+ KL V  +DVKNVIIWGNHSS+QYPDVNHA V     E  V E +KDD+WL GEF+TTVQQRGAA+IKARK           CDH+RD   GTPEG +VSMGV SDG SY VP  L+
Sbjct:  132 PANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLL 285          
BLAST of CN188428 vs. ExPASy Swiss-Prot
Match: MDHC_CHICK (Malate dehydrogenase, cytoplasmic OS=Gallus gallus GN=MDH1 PE=2 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 1.744e-44
Identity = 93/154 (60.39%), Postives = 106/154 (68.83%), Query Frame = 2
Query:    2 PXIXNXLILKEFAPSIPAKIFTSLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPEGTWVSMGVYSDG-SYNVPAGLI 460
            P   N LI  + APSIP + F+ LTRLDHNRA  QI+ KL V  +DVKNVIIWGNHSS+QYPDVNHA VN    E  V E +KDD+WL G+FI TVQQRGAA+IKARK           CDH+RD   GTP G +VSMGV SDG SY VP  L+
Sbjct:  132 PANTNCLIASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTSNDVKNVIIWGNHSSTQYPDVNHAKVNVKGKEVGVYEAIKDDSWLKGDFILTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPAGEFVSMGVISDGNSYGVPEDLL 285          
The following BLAST results are available for this feature:
BLAST of CN188428 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 187
Match NameE-valueIdentityDescription
MDHC_MEDSA2.117e-6680.39Malate dehydrogenase, cytoplasmic OS=Medicago sati... [more]
MDHC_MAIZE2.117e-6681.70Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2... [more]
MDHC_MESCR6.159e-6680.39Malate dehydrogenase, cytoplasmic OS=Mesembryanthe... [more]
MDHC_BETVU6.159e-6681.05Malate dehydrogenase, cytoplasmic OS=Beta vulgaris... [more]
MDHC2_ARATH5.214e-6579.08Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis... [more]
MDHC_ORYSJ1.981e-6479.74Malate dehydrogenase, cytoplasmic OS=Oryza sativa ... [more]
MDHC1_ARATH1.981e-6479.08Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis... [more]
MDHC_PIG4.589e-4561.69Malate dehydrogenase, cytoplasmic OS=Sus scrofa GN... [more]
MDHC_HUMAN1.335e-4460.39Malate dehydrogenase, cytoplasmic OS=Homo sapiens ... [more]
MDHC_CHICK1.744e-4460.39Malate dehydrogenase, cytoplasmic OS=Gallus gallus... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006A09_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006A09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188428 ID=CN188428; Name=CN188428; organism=Citrus sinensis; type=EST; length=461bp
CCCTGNNATTATNAATGNATTGATTCTGAAAGAATTTGCGCCATCAATCC
CTGCTAAGATCTTCACAAGCTTGACCAGATTGGATCATAACAGGGCATTG
GGACAAATATCTGAGAAACTGAATGTCCAAGTATCTGATGTTAAGAATGT
GATTATTTGGGGAAATCATTCATCATCTCAGTACCCTGATGTCAACCATG
CAACTGTCAATACTGCAGCTGGGGAAAAGCCTGTCCGTGAGCTTGTCAAG
GATGATGCATGGTTGAATGGAGAATTCATAACCACAGTCCAACAACGTGG
TGCTGCAATCATCAAAGCCAGAAAGCTCTCGAGTGCTCTGTCTGCTGCTA
GCTCTGCTTGTGACCATATCCGTGATTGGGTTCTTGGAACTCCTGAGGGC
ACTTGGGTCTCCATGGGGGTATACTCTGATGGTTCATACAATGTACCAGC
TGGGCTCATTT
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