CK936719

Overview
NameCK936719
Unique NameCK936719
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length829
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: METC_ARATH (Cystathionine beta-lyase, chloroplastic OS=Arabidopsis thaliana GN=At3g57050 PE=1 SV=1)

HSP 1 Score: 325.094 bits (832), Expect = 2.990e-88
Identity = 177/273 (64.84%), Postives = 203/273 (74.36%), Query Frame = 1
Query:   28 AYTDLTNSGSSIPLNSPTSFGFSK------RRIIFAKELKLNCSIDREKEIDVSTSALVDDVAXXXXXXXXXXXXPGVSTLLMNFSNEFDPYGALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            ++ DL++ G  I  NSPTS   SK      ++I      KLNC +  EK +D  T + V++                VSTLL+N  N+FDP+ A+STPLYQTATFKQPSA ENG YDYTRSGNPTRDALES            CFTSGMAAL+AVTHL+  GEEIVAGDD+YGG+DRLLS+V P++GVVVKRVNT  LDEVA+AIGP TKLVW+ESPTNPRQQI DIRKI+EMAHA GAL+LVDNSIMSPVLSRPLELGADIVMHSATKFIAG
Sbjct:   14 SFADLSDRGL-ISKNSPTSVSISKVPTWEKKQISNRNSFKLNCVM--EKSVDGQTHSTVNNTTDSLNTMNIKEEA-SVSTLLVNLDNKFDPFDAMSTPLYQTATFKQPSAIENGPYDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 282          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: METC_LACLM (Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=metC PE=1 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.361e-40
Identity = 80/181 (44.20%), Postives = 119/181 (65.75%), Query Frame = 1
Query:  286 GALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            GA+S P+YQT+T+KQ    +   Y+Y+RSGNPTR ALE              F+SG+A + AV  L   G+ I+  DD+YGGT RL+ +V  KTG++   V+  +LD++ +A    TK ++ E+P+NP  ++ DI++I+ +A AH AL LVDN+  +P L +P+ LGADIV+HSATK++ G
Sbjct:   20 GAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELIADLEGGVQGFAFSSGLAGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAIAKAHDALTLVDNTFATPYLQQPIALGADIVLHSATKYLGG 200          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: METC_LACLA (Cystathionine beta-lyase OS=Lactococcus lactis subsp. lactis GN=metC PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.361e-40
Identity = 80/181 (44.20%), Postives = 119/181 (65.75%), Query Frame = 1
Query:  286 GALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            GA+S P+YQT+T+KQ    +   Y+Y+RSGNPTR ALE              F+SG+A + AV  L   G+ I+  DD+YGGT RL+ +V  KTG++   V+  +LD++ +A    TK ++ E+P+NP  ++ DI++I+ +A AH AL LVDN+  +P L +P+ LGADIV+HSATK++ G
Sbjct:   20 GAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELIADLEGGVQGFAFSSGLAGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYFETPSNPLLKVLDIKEISAIAKAHDALTLVDNTFATPYLQQPIALGADIVLHSATKYLGG 200          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: METC_LACLC (Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris GN=metC PE=1 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 2.322e-40
Identity = 80/181 (44.20%), Postives = 119/181 (65.75%), Query Frame = 1
Query:  286 GALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            GA+S P+YQT+T+KQ    +   Y+Y+RSGNPTR ALE              F+SG+A + AV  L   G+ I+  DD+YGGT RL+ +V  KTG++   V+  +L+++ +A    TK V+ E+P+NP  ++ DI++I+ +A AH AL LVDN+  +P L +P+ LGADIV+HSATK++ G
Sbjct:   20 GAVSVPIYQTSTYKQNGLGQPKEYEYSRSGNPTRHALEELIADLEGGVQGFAFSSGLAGIHAVLSLFSAGDHIILADDVYGGTFRLVDKVLTKTGIIYDLVDLSNLEDLKAAFKAETKAVYFETPSNPLLKVLDIKEISSIAKAHNALTLVDNTFATPYLQQPIALGADIVLHSATKYLGG 200          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: MCCB_BACSU (Cystathionine gamma-lyase OS=Bacillus subtilis GN=mccB PE=1 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 3.960e-40
Identity = 80/181 (44.20%), Postives = 120/181 (66.30%), Query Frame = 1
Query:  286 GALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            GA+S P+YQ +T+KQP A ++  Y+Y+R+ NPTR ALE+             F+SGMAA+ AV  L  +G+ +V  DD+YGGT R++++V  + G+    V+T   +EV  AI P TK +++E+PTNP  +I D+  +A++A   G LL+VDN+  +P   +PL LGADIV+HSATK++ G
Sbjct:   19 GAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVTELESGEAGYAFSSGMAAITAVMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVDTSSREEVEKAIRPNTKAIYIETPTNPLLKITDLTLMADIAKKAGVLLIVDNTFNTPYFQQPLTLGADIVLHSATKYLGG 199          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: CBL_SCHPO (Cystathionine beta-lyase OS=Schizosaccharomyces pombe GN=str3 PE=2 SV=4)

HSP 1 Score: 162.925 bits (411), Expect = 1.965e-39
Identity = 90/198 (45.45%), Postives = 120/198 (60.61%), Query Frame = 1
Query:  241 VSTLLMNFSNEFDPYGALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEM--AHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            V T L++     D Y A S P+YQ+ATFKQP     G +DYTRSGNPTR  L+               ++GM+AL  +     +   +VAG DLYGG+DRLLS    + G  V  V+T DL    +A+ P T LV +ESPTNPR  ICDIR I ++  + A  ALL++DN+++SPVL  PL+ G DIV  SATK+++G
Sbjct:    9 VDTELVHVEGNEDQYHASSVPIYQSATFKQPCLEHMGKFDYTRSGNPTRSVLQVHLAKLMKAKHAFVTSNGMSALDMILRCCKSNSHVVAGHDLYGGSDRLLSFNQRQYGFKVDNVDTSDLAAFEAALRPDTNLVLIESPTNPRISICDIRAIVKITRSKAKDALLVMDNTMLSPVLCNPLDFGYDIVYESATKYLSG 206          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: CGL_DICDI (Cystathionine gamma-lyase OS=Dictyostelium discoideum GN=cysA PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 2.567e-39
Identity = 81/182 (44.51%), Postives = 113/182 (62.09%), Query Frame = 1
Query:  286 GALSTPLYQTATFKQPS-ATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            GA+  P+  + TF QPS    +  YDY+RSGNPTR A E              F SG+A L  +THLL +G+E+++ DD+YGGT R  +RV     +    V+   LD++ +A    T+LVW+E+PTNP  ++ DI+ +A+  H+ GA L+VDN+ MSP    PL+LGADIVMHS TK+I G
Sbjct:   25 GAVIVPISLSTTFLQPSPGVLHSEYDYSRSGNPTRKAFEECIAACENAKYALSFASGLATLTTITHLLKSGDEVISIDDVYGGTRRYFTRVAANFDLKFSFVDLSTLDDLKNAFTDKTRLVWIETPTNPLLKVADIKAVADYVHSRGATLVVDNTFMSPYFQNPLDLGADIVMHSVTKYING 206          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: METC_BACSU (Cystathionine beta-lyase metC OS=Bacillus subtilis GN=metC PE=1 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.274e-38
Identity = 79/181 (43.65%), Postives = 117/181 (64.64%), Query Frame = 1
Query:  286 GALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            GA+S P+   +TF Q S  E GAYDY+RSG PTR ALE              F+SGMAA++    LL  G+ ++  +D+YGGT R+++ V  + G+    V+  D +EVA +I P TK++++E+P+NP   I DI+ + ++A  +G L  +DN+ M+P L RPL+LG DIV+HSATKF++G
Sbjct:   24 GAVSVPIQHASTFHQSSFEEFGAYDYSRSGTPTRTALEETIAALEGGTRGFAFSSGMAAISTAFLLLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIEHTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKAVVQLAKENGCLTFLDNTFMTPALQRPLDLGVDIVLHSATKFLSG 204          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: METB_HELPY (Cystathionine gamma-synthase OS=Helicobacter pylori GN=metB PE=3 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 6.322e-38
Identity = 77/181 (42.54%), Postives = 115/181 (63.54%), Query Frame = 1
Query:  286 GALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHGALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            GA+S P+YQT+T++Q +   +  Y+Y+RSGNPTR ALE              F SG+A + AV  LL +G+ ++ GDD+YGGT RL ++V  K G+    ++T D+ ++  AI P TK +++E+P+NP  +I D+ + A +A  HG L +VDN+  +P    PL LGADIV HS TK++ G
Sbjct:   19 GAVSVPIYQTSTYRQDAIGRHKGYEYSRSGNPTRFALEELIADLEGGVKGFAFASGLAGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNQVLVKNGLSCTIIDTSDISQIKKAIKPNTKALYLETPSNPLLKITDLAQCASVAKDHGLLTIVDNTFATPYYQNPLLLGADIVAHSGTKYLGG 199          
BLAST of CK936719 vs. ExPASy Swiss-Prot
Match: CGL_HUMAN (Cystathionine gamma-lyase OS=Homo sapiens GN=CTH PE=1 SV=3)

HSP 1 Score: 157.532 bits (397), Expect = 8.257e-38
Identity = 78/181 (43.09%), Postives = 114/181 (62.98%), Query Frame = 1
Query:  289 ALSTPLYQTATFKQPSATENGAYDYTRSGNPTRDALESXXXXXXXXXXXXCFTSGMAALAAVTHLLGTGEEIVAGDDLYGGTDRLLSRVTPKTGVVVKRVNTCDLDEVASAIGPWTKLVWVESPTNPRQQICDIRKIAEMAHAHG-ALLLVDNSIMSPVLSRPLELGADIVMHSATKFIAG 828
            A+  P+  + TFKQ +  ++  ++Y+RSGNPTR+ LE              F SG+AA   +THLL  G++I+  DD+YGGT+R   +V  + G+ +  V+   +  + +AI P TKLVW+E+PTNP Q++ DI   A + H HG  +L+VDN+ MSP   RPL LGADI M+SATK++ G
Sbjct:   36 AVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNG 216          
The following BLAST results are available for this feature:
BLAST of CK936719 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 39
Match NameE-valueIdentityDescription
METC_ARATH2.990e-8864.84Cystathionine beta-lyase, chloroplastic OS=Arabido... [more]
METC_LACLM1.361e-4044.20Cystathionine beta-lyase OS=Lactococcus lactis sub... [more]
METC_LACLA1.361e-4044.20Cystathionine beta-lyase OS=Lactococcus lactis sub... [more]
METC_LACLC2.322e-4044.20Cystathionine beta-lyase OS=Lactococcus lactis sub... [more]
MCCB_BACSU3.960e-4044.20Cystathionine gamma-lyase OS=Bacillus subtilis GN=... [more]
CBL_SCHPO1.965e-3945.45Cystathionine beta-lyase OS=Schizosaccharomyces po... [more]
CGL_DICDI2.567e-3944.51Cystathionine gamma-lyase OS=Dictyostelium discoid... [more]
METC_BACSU1.274e-3843.65Cystathionine beta-lyase metC OS=Bacillus subtilis... [more]
METB_HELPY6.322e-3842.54Cystathionine gamma-synthase OS=Helicobacter pylor... [more]
CGL_HUMAN8.257e-3843.09Cystathionine gamma-lyase OS=Homo sapiens GN=CTH P... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004519_A01 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IIF_A01 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936719 ID=CK936719; Name=CK936719; organism=Citrus sinensis; type=EST; length=829bp
GGGGATCTCAAGCCTTTCGTCTCCGATGCCTACACTGATCTCACCAACAG
TGGTTCTTCAATACCTTTAAATTCCCCAACAAGCTTTGGGTTTAGTAAAA
GGCGGATAATATTTGCGAAGGAATTAAAACTGAATTGCTCGATAGACAGG
GAAAAGGAAATTGATGTCAGCACTTCAGCTCTTGTGGATGATGTAGCTGA
GTGTTTGGAAGAAGAAATGAGCGTGGAGGAGGAGCCTGGTGTTTCTACAT
TATTGATGAATTTTTCTAATGAGTTTGATCCTTATGGTGCGTTGAGCACT
CCGCTTTATCAGACTGCGACATTTAAGCAACCTTCTGCGACAGAGAATGG
CGCCTATGATTATACCAGAAGTGGAAATCCTACACGAGATGCCTTAGAAA
GCCTCCTTGCAAAGCTTGATAAGGCCGACCAAGCGCTTTGCTTCACCAGC
GGAATGGCTGCTTTGGCTGCTGTTACTCATCTTCTTGGAACTGGTGAAGA
AATTGTTGCTGGAGATGACCTGTATGGTGGTACTGATCGTTTGTTATCAC
GAGTAACTCCTAAAACTGGGGTTGTAGTGAAGCGTGTGAATACATGCGAT
CTAGATGAGGTTGCCTCTGCTATTGGTCCTTGGACAAAGCTTGTCTGGGT
GGAAAGTCCAACCAACCCTCGACAACAAATTTGTGACATTCGTAAAATTG
CCGAAATGGCTCATGCACATGGTGCTCTTCTGCTAGTGGATAACAGCATT
ATGTCCCCTGTATTATCTCGACCTCTGGAACTCGGAGCAGACATTGTGAT
GCACTCAGCTACTAAATTTATAGCTGGAC
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