CX674772

Overview
NameCX674772
Unique NameCX674772
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length809
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACO2M_ARATH (Aconitate hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=ACO2 PE=1 SV=2)

HSP 1 Score: 234.572 bits (597), Expect = 5.084e-61
Identity = 117/155 (75.48%), Postives = 130/155 (83.87%), Query Frame = 2
Query:  356 AVRSFRCSVP---RWSHRVDWR-SPLSLRAQIRTVAPAIERLERAFATMAAEHPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            A RSF    P   RWSH    + SP    +QIR V+P ++RL+R F++MA+EHPFK I T LPKPGGGEFGKFYSLPALNDPR++KLPYSIRILLESAIRNCDNFQV KEDVEKIIDWE ++PKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA
Sbjct:   45 ARRSFGTISPAFRRWSHSFHSKPSPFRFTSQIRAVSPVLDRLQRTFSSMASEHPFKGIFTTLPKPGGGEFGKFYSLPALNDPRVDKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 199          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACO3M_ARATH (Aconitate hydratase 3, mitochondrial OS=Arabidopsis thaliana GN=ACO3 PE=1 SV=2)

HSP 1 Score: 234.187 bits (596), Expect = 6.640e-61
Identity = 113/156 (72.44%), Postives = 134/156 (85.90%), Query Frame = 2
Query:  347 FASAVRSFR-CSVP-RWSHRVDWRSPLSLRAQIRTVAPAIERLERAFATMAAEHPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            F+SA+RSFR CS   RWSH   W SP SLRAQ R   P +E+ ER +ATMA+EH +K+ILT+LPKPGGGE+GK+YSLPALNDPRI+KLP+S+RILLESAIRNCDN+QV K+DVEKI+DWEN++ KQVEI FKPARV+LQDFTGVP +VDLA MRDA
Sbjct:   49 FSSALRSFRVCSASTRWSHGGSWGSPASLRAQARNSTPVMEKFERKYATMASEHSYKDILTSLPKPGGGEYGKYYSLPALNDPRIDKLPFSVRILLESAIRNCDNYQVTKDDVEKILDWENTSTKQVEIAFKPARVILQDFTGVPVLVDLASMRDA 204          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACOC_CUCMA (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 6.655e-53
Identity = 99/107 (92.52%), Postives = 106/107 (99.07%), Query Frame = 2
Query:  488 MAAEHPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            MAAE+PFKE LT+LPKPGGGEFGK+YSLP+LNDPRI++LPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENS+PKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA
Sbjct:    1 MAAENPFKENLTSLPKPGGGEFGKYYSLPSLNDPRIDRLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSSPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 107          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACOC_ORYSJ (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 198.749 bits (504), Expect = 3.091e-50
Identity = 95/107 (88.79%), Postives = 100/107 (93.46%), Query Frame = 2
Query:  488 MAAEHPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            MAAEHPFK ILT LPKPGGGE+GKFYSLPALNDPRI+KLPYSIRILLESAIRNCDNFQV + DVEKIIDWEN++PK  EIPFKPARVLLQDFTGVPAVVDLA MRDA
Sbjct:    1 MAAEHPFKNILTTLPKPGGGEYGKFYSLPALNDPRIDKLPYSIRILLESAIRNCDNFQVNQNDVEKIIDWENTSPKLAEIPFKPARVLLQDFTGVPAVVDLAAMRDA 107          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACO1_ARATH (Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2)

HSP 1 Score: 191.815 bits (486), Expect = 3.779e-48
Identity = 91/107 (85.05%), Postives = 100/107 (93.46%), Query Frame = 2
Query:  488 MAAEHPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            MA+E+PF+ IL AL KP GGEFG +YSLPALNDPRI+KLPYSIRILLESAIRNCD FQVK +DVEKI+DWEN++PKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA
Sbjct:    1 MASENPFRSILKALEKPDGGEFGNYYSLPALNDPRIDKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 107          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACOC_DICDI (Probable cytoplasmic aconitate hydratase OS=Dictyostelium discoideum GN=aco1 PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 1.105e-31
Identity = 71/107 (66.36%), Postives = 83/107 (77.57%), Query Frame = 2
Query:  488 MAAEHPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            M   +PF ++   L      +   FY+L  L DPRIEKLPYSIRILLESA+RNCDNF+V ++DVE I++WEN+A K VEIPFKPARVLLQDFTGVPAVVDLA MRDA
Sbjct:    1 MTTNNPFDKVKDVLKSQD--QTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNCDNFEVHEKDVENILNWENTANK-VEIPFKPARVLLQDFTGVPAVVDLAAMRDA 104          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACOC_CHICK (Cytoplasmic aconitate hydratase OS=Gallus gallus GN=ACO1 PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 2.384e-26
Identity = 60/103 (58.25%), Postives = 75/103 (72.82%), Query Frame = 2
Query:  500 HPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            +PF +I+   P        KF++L  L D R  +LP+SIR+LLE+AIRNCD F VKK+DVE I++W+    K VE+PFKPARV+LQDFTGVPAVVD A MRDA
Sbjct:    3 NPFVQIVE--PLDAKEPVKKFFNLSKLEDVRYARLPFSIRVLLEAAIRNCDEFLVKKQDVENILNWKVMQHKNVEVPFKPARVILQDFTGVPAVVDFAAMRDA 103          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACOC_CAEEL (Probable cytoplasmic aconitate hydratase OS=Caenorhabditis elegans GN=aco-1 PE=1 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.113e-26
Identity = 57/89 (64.04%), Postives = 68/89 (76.40%), Query Frame = 2
Query:  542 GGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            G    K++ L  LND R  +LP SI+ LLE+A+R+CD F V K+DVE I+DW+NS   Q EIPFKPARV+LQDFTGVPAVVDLA MRDA
Sbjct:   13 GDNVYKYFDLNGLNDARYNELPISIKYLLEAAVRHCDEFHVLKKDVETILDWKNSQRNQAEIPFKPARVILQDFTGVPAVVDLAAMRDA 101          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACOC_BOVIN (Cytoplasmic aconitate hydratase OS=Bos taurus GN=ACO1 PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 4.066e-26
Identity = 59/103 (57.28%), Postives = 74/103 (71.84%), Query Frame = 2
Query:  500 HPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            +PF  ++  L     G+  KF++L  L D R   LP+SIR+LLE+AIRNCD F VKK DVE I++W+    K +E+PFKPARV+LQDFTGVPAVVD A MRDA
Sbjct:    3 NPFAHLVEPLDPAQPGK--KFFNLNKLEDSRYGSLPFSIRVLLEAAIRNCDQFLVKKNDVENILNWKVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDA 103          
BLAST of CX674772 vs. ExPASy Swiss-Prot
Match: ACOC_HUMAN (Cytoplasmic aconitate hydratase OS=Homo sapiens GN=ACO1 PE=1 SV=3)

HSP 1 Score: 118.242 bits (295), Expect = 5.310e-26
Identity = 58/103 (56.31%), Postives = 74/103 (71.84%), Query Frame = 2
Query:  500 HPFKEILTALPKPGGGEFGKFYSLPALNDPRIEKLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSAPKQVEIPFKPARVLLQDFTGVPAVVDLACMRDA 808
            +PF  +   L     G+  KF++L  L D R  +LP+SIR+LLE+AIRNCD F VKK+D+E I+ W  +  K +E+PFKPARV+LQDFTGVPAVVD A MRDA
Sbjct:    3 NPFAHLAEPLDPVQPGK--KFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDA 103          
The following BLAST results are available for this feature:
BLAST of CX674772 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 46
Match NameE-valueIdentityDescription
ACO2M_ARATH5.084e-6175.48Aconitate hydratase 2, mitochondrial OS=Arabidopsi... [more]
ACO3M_ARATH6.640e-6172.44Aconitate hydratase 3, mitochondrial OS=Arabidopsi... [more]
ACOC_CUCMA6.655e-5392.52Aconitate hydratase, cytoplasmic OS=Cucurbita maxi... [more]
ACOC_ORYSJ3.091e-5088.79Putative aconitate hydratase, cytoplasmic OS=Oryza... [more]
ACO1_ARATH3.779e-4885.05Aconitate hydratase 1 OS=Arabidopsis thaliana GN=A... [more]
ACOC_DICDI1.105e-3166.36Probable cytoplasmic aconitate hydratase OS=Dictyo... [more]
ACOC_CHICK2.384e-2658.25Cytoplasmic aconitate hydratase OS=Gallus gallus G... [more]
ACOC_CAEEL3.113e-2664.04Probable cytoplasmic aconitate hydratase OS=Caenor... [more]
ACOC_BOVIN4.066e-2657.28Cytoplasmic aconitate hydratase OS=Bos taurus GN=A... [more]
ACOC_HUMAN5.310e-2656.31Cytoplasmic aconitate hydratase OS=Homo sapiens GN... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS10_8F02_g Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10 Citrus sinensis cDNA clone UCRCS10-8F02-L4-5.g, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX674772 ID=CX674772; Name=CX674772; organism=Citrus sinensis; type=EST; length=809bp
GCCCTCTCACTCTCTCTCTTTCATCTAAAAATAACAGAACAGAAGACGCC
ATGAATTGAAAGAGCATTATTGCCATCAACCAACCAATAATCAAATCCAT
CTGAGACTCTGTACCAAATCCTTATCTCTTTTTTTGGCCTTTCCGTTTTG
TGTGAATGCGTGATCCAAGACATATACGTACCTCGCCATCGTCATGTATA
TAACAACCTCCACCTCAGCAGCTTCTTCAATCCTCAGAGCTTCTTCTAGG
TCACGATTCGTCTCTTCGCTATCTTCATTTAAGTCCTTACCTGCACGTTC
TCTTTCTCCTTCTCCTTCATCGTTGGTCTCTCAGCGATCTCTCGGCTTCG
CCTCGGCCGTTCGATCCTTCCGCTGCTCCGTCCCTCGCTGGAGCCACCGC
GTTGACTGGCGCTCTCCGCTTAGTCTTCGCGCTCAGATCAGAACCGTTGC
TCCTGCCATCGAACGGCTCGAACGCGCCTTTGCTACTATGGCTGCTGAAC
ATCCATTCAAGGAGATCTTGACCGCTCTTCCAAAGCCAGGAGGTGGCGAG
TTTGGAAAGTTCTATAGCCTTCCTGCACTCAATGATCCAAGAATTGAGAA
GCTGCCATACTCTATCAGAATCCTGCTTGAATCTGCTATTCGTAATTGTG
ACAACTTTCAAGTTAAGAAGGAAGATGTCGAGAAAATTATTGACTGGGAA
AATTCTGCACCAAAGCAAGTTGAAATTCCCTTCAAGCCTGCTCGTGTATT
GTTGCAGGATTTTACTGGAGTACCAGCCGTGGTTGACCTTGCCTGCATGC
GTGATGCTA
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