CV885281

Overview
NameCV885281
Unique NameCV885281
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length847
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Library UCRCS04-UCRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS2_PONAB (Fatty acid desaturase 2 OS=Pongo abelii GN=FADS2 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.241e-22
Identity = 69/233 (29.61%), Postives = 108/233 (46.35%), Query Frame = 1
Query:  154 QSAALMEAEKKYITAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYHPGTAW--QYLDKLFTGYFV-----QDF-EVSEISKDYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGVLCCESVW-AHLGSGMLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAV 825
            + AA  E      + EE++ HN   D W+ I  KVY++T+W+ QHPGG   + + AG+D TDAF A+HP   +  ++L  L  G        QD  + S+I++D+R L        +F+         L  +  +  +  + V    + W + L +  +L     Q+ ++ HD GH  V   PK+N +        L G S  WW   H  HH   N    DPD+  + VF +
Sbjct:    9 EGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWISTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVL 241          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS2_HUMAN (Fatty acid desaturase 2 OS=Homo sapiens GN=FADS2 PE=1 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.927e-22
Identity = 74/244 (30.33%), Postives = 114/244 (46.72%), Query Frame = 1
Query:  154 QSAALMEAEKKYITAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYHPGTAW--QYLDKLFTGYFV-----QDF-EVSEISKDYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGVLCCESV-W---AHLGSG--------MLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAV 825
            + AA  E      + EE++ HN   D W+ I  KVY++T+W+ QHPGG   + + AG+D TDAF A+HP   +  ++L  L  G        QD  + S+I++D+R L        +F K  HV           F++++  ++  ES+ W    + G+G         +L     Q+ ++ HD GH  V   PK+N +        L G S  WW   H  HH   N    DPD+  + VF +
Sbjct:    9 EGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLF-KTNHV----------FFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVL 241          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS2_MACFA (Fatty acid desaturase 2 OS=Macaca fascicularis GN=FADS2 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.823e-22
Identity = 74/244 (30.33%), Postives = 113/244 (46.31%), Query Frame = 1
Query:  154 QSAALMEAEKKYITAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYHPGTAW--QYLDKLFTGYFV-----QDF-EVSEISKDYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGVLCCESV-W---AHLGSG--------MLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAV 825
            + AA  E      + EE++ HN   D W+ I  KVY++T+W+ QHPGG   + + AG+D TDAF A+HP   +  ++L  L  G        QD  + S+I +D+R L        +F K  HV           F++++  ++  ES+ W    + G+G         +L     Q+ ++ HD GH  V   PK+N +        L G S  WW   H  HH   N    DPD+  + VF +
Sbjct:    9 EGAAEREVPMPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSTQHPGGQRVIGHYAGEDATDAFRAFHPDLKFVGKFLKPLLIGELAPEEPSQDHGKNSKIIEDFRALKKTAEDMNLF-KTNHV----------FFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVL 241          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS1_PAPAN (Fatty acid desaturase 1 OS=Papio anubis GN=FADS1 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 3.236e-21
Identity = 66/239 (27.62%), Postives = 112/239 (46.86%), Query Frame = 1
Query:  145 PIFQSAALMEAEKKYITAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYH--PGTAWQYLDKLFTGYFVQD---FEVS---EISKDYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGV------LCCESVWAHLGSGMLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVF 819
            P+     +     +Y T +E+   +  E+ W+ I  KVYD++E+ ++HPGG   + + AGQD TD F+A+H   G   +Y++ L  G    +   FE +   E++ ++R L     + G+  K  HV       + L+ ++++ G       +   S    L   +LL    IQ+ ++ HD GH  V ++ K+N +        L G+  +WW   H  HH   N    DPD+   P F
Sbjct:    5 PVAAKTPVQGPTPRYFTWDEVAQRSGCEERWLVIDRKVYDISEFTRRHPGGSRVISHYAGQDATDPFVAFHSNKGLVKKYMNSLLIGELSPEQPSFEPTKNKELTDEFRELRATVEQMGLM-KANHV----FFLLYLLHILLLDGAAWLTLWIFGTSFLPFLLCAVLLTAAQIQAGWLQHDLGHLSVFSTSKWNHLVHHFVIGHLKGVPASWWNHMHFQHHAKPNCFGKDPDINMHPFF 238          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS2_DANRE (Fatty acid desaturase 2 OS=Danio rerio GN=fads2 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.227e-21
Identity = 65/222 (29.28%), Postives = 101/222 (45.50%), Query Frame = 1
Query:  193 TAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYHPGTAWQYLDKLFTGYFVQDFEVSEIS----------KDYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGVLCCESVWAH-LGSGMLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAV 825
            T EE++ H K  D W+ ++ KVY+V++W K+HPGG   L + AG+D T+AF A+HP    Q + K      + + E SE S          +D+R L      +G F+ +    +  L  + L+  +    V    + W + L   ++L     Q+ ++ HD GH  V  +   N +        L G S  WW   H  HH   N    DPD+  +  F V
Sbjct:   22 TWEEVQKHTKHGDQWVVVERKVYNVSQWVKRHPGGLRILGHYAGEDATEAFTAFHPNL--QLVRKYLKPLLIGELEASEPSQDRQKNAALVEDFRALRERLEAEGCFKTQPLFFALHLGHILLLEAIAFMMVWYFGTGWINTLIVAVILATAQSQAGWLQHDFGHLSVFKTSGMNHLVHKFVIGHLKGASAGWWNHRHFQHHAKPNIFKKDPDVNMLNAFVV 241          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS2_XENLA (Fatty acid desaturase 2 OS=Xenopus laevis GN=fads2 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.606e-20
Identity = 65/229 (28.38%), Postives = 104/229 (45.41%), Query Frame = 1
Query:  199 EELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYHPGTAW--QYLDKLFTGYFVQDFEVSEISK------DYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGVLCCESV-WA---HLGSG--------MLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAV 825
            EE++ HN   D W+ I+ KVY++++W K+HPGG   + + AG+D TDAF A+HP   +  ++L  L+ G   ++    +  K      D+R L        +F+                F+  ++ +L  E + W    +LG+G        +LL     Q+ ++ HD GH  V    K+N +        L G S  WW   H  HH   N    DPD+  + VF +
Sbjct:   26 EEIQKHNLKTDKWLVIERKVYNISQWVKRHPGGMRIIGHYAGEDATDAFHAFHPDKTFVRKFLKPLYIGELAENEPSQDRDKNAQQVEDFRALRKTAEDMRLFKSN-----------PAFFIFYLFHILLIEFLAWCTLHYLGTGWIPAIITVLLLTISQAQAGWLQHDFGHLSVFEKSKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFSKDPDVNMVNVFVL 243          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS2_BOVIN (Fatty acid desaturase 2 OS=Bos taurus GN=FADS2 PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 2.098e-20
Identity = 63/233 (27.04%), Postives = 105/233 (45.06%), Query Frame = 1
Query:  154 QSAALMEAEKKYITAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYHPGTAW--QYLDKLFTGYFVQD------FEVSEISKDYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGVLCCESVW-AHLGSGMLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAV 825
            + A  +EA       EE++ HN   D W+ I  KVY++T+W+ +HPGG   + + AG+D TDAF+A+H    +  +++  L  G    +       + S+I++D+R L        +F+  Q      L  +  M  +  + +    + W   + +  +L     Q+ ++ HD GH  V     +N I        L G S  WW   H  HH   N    DPD+  + VF +
Sbjct:    9 EGATELEAPMPTFRWEEIQKHNLRTDKWLVIDRKVYNITKWSSRHPGGQRVIGHYAGEDATDAFLAFHRNLDFVRKFMKPLLIGELAPEEPSQDRGKNSQITEDFRALRKTAEDMNLFKSNQLFFLLHLAHIIAMESIAWFTLFYFGNGWIPTIITAFVLATSQAQAGWLQHDYGHLSVYKKSMWNHIVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVL 241          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS3_BOVIN (Fatty acid desaturase 3 OS=Bos taurus GN=FADS3 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.740e-20
Identity = 61/216 (28.24%), Postives = 100/216 (46.30%), Query Frame = 1
Query:  199 EELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYHPGTAW--QYLDKLFTGYFVQDF------EVSEISKDYRRLYIEFAKQGMFEKKQHVASCALTCVALMFVVVVYGVLCCESVWA-HLGSGMLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVF 819
            E++R HN   D W+ I+ +VYD++ WA++HPGG   + +   +D TDAF A+H   ++  ++L  L  G    +       + +++ +D+R L        +FE K       L  +  M V+    +      W     + ++L     QS  + HD GH  +  + ++N +AQ      L G S  WW + H  HH   N    DPD+   PVF
Sbjct:   24 EQVRRHNLPGDKWLVIERRVYDISRWAQRHPGGSRLIGHHGAEDATDAFHAFHQDLSFVRKFLQPLLIGELAPEEPSQDGPQNTQLIEDFRALRQAVEDMKLFEAKPAFFGLLLGHILAMEVLAWLMIYMLGPGWVPSTLAALILAISQAQSWCLQHDLGHTSIFRNSRWNHLAQQFVMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPVF 239          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FADS1_HUMAN (Fatty acid desaturase 1 OS=Homo sapiens GN=FADS1 PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.740e-20
Identity = 64/238 (26.89%), Postives = 108/238 (45.38%), Query Frame = 1
Query:  145 PIFQSAALMEAEKKYITAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYH--PGTAWQYLDKLFTGYFVQD---FEVS---EISKDYRRLYIEFAKQGMFEKKQ-----HVASCALTCVALMFVVVVYGVLCCESVWAHLGSGMLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVF 819
            P+    A      +Y T +E+   +  E+ W+ I  KVY+++E+ ++HPGG   + + AGQD TD F+A+H   G   +Y++ L  G    +   FE +   E++ ++R L     + G+ +        ++    L   A    + V+G     S    L   +LL  +  Q+ ++ HD GH  V ++ K+N +        L G   +WW   H  HH   N    DPD+   P F
Sbjct:    5 PVAAETAAQGPTPRYFTWDEVAQRSGCEERWLVIDRKVYNISEFTRRHPGGSRVISHYAGQDATDPFVAFHINKGLVKKYMNSLLIGELSPEQPSFEPTKNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTLWVFGT----SFLPFLLCAVLLSAVQAQAGWLQHDFGHLSVFSTSKWNHLLHHFVIGHLKGAPASWWNHMHFQHHAKPNCFRKDPDINMHPFF 238          
BLAST of CV885281 vs. ExPASy Swiss-Prot
Match: FAD2L_MOUSE (Fatty acid desaturase 2-like protein OS=Mus musculus PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.740e-20
Identity = 69/225 (30.67%), Postives = 101/225 (44.89%), Query Frame = 1
Query:  193 TAEELRTHNKAEDLWISIQGKVYDVTEWAKQHPGGDVPLLNLAGQDVTDAFIAYH--PGTAWQYLDKLFTGYFVQDFEVSEISK------DYRRLYIEFAKQGMFEKK-----QHVASCALTCVALMFVVVVYGVLCCESVW-AHLGSGMLLGFLWIQSAYVGHDSGHYQVMTSPKFNKIAQLISGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAV 825
            T +E++ H++  D W+ I  KVY+VT+WA +HPGG   L + AGQD TDAF A H   G    YL  L  G    +    E +K      D+R L        MF         H+A   +  ++   ++  +G     S W   +    LL     Q +++ HD GH  +    K+N +        L G+S  WW + H  HH+  N    DPD+   P+F V
Sbjct:   66 TWQEIQRHSQEADQWLVIDRKVYNVTDWAGKHPGGRRVLNHYAGQDATDAFRAMHLDLGMVKLYLKPLLIGELSPEEPSQEKNKNAQLVEDFRELRKTLEAMNMFSANLRFFFLHLAQILILEISAWLILHHFG-----SSWLVTILISFLLTVSQAQCSFLQHDLGHLSMFKKSKWNHLMHKFVMCHLKGLSADWWNYRHFQHHVKPNIYPKDPDIDVGPLFLV 285          
The following BLAST results are available for this feature:
BLAST of CV885281 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 24
Match NameE-valueIdentityDescription
FADS2_PONAB2.241e-2229.61Fatty acid desaturase 2 OS=Pongo abelii GN=FADS2 P... [more]
FADS2_HUMAN2.927e-2230.33Fatty acid desaturase 2 OS=Homo sapiens GN=FADS2 P... [more]
FADS2_MACFA3.823e-2230.33Fatty acid desaturase 2 OS=Macaca fascicularis GN=... [more]
FADS1_PAPAN3.236e-2127.62Fatty acid desaturase 1 OS=Papio anubis GN=FADS1 P... [more]
FADS2_DANRE4.227e-2129.28Fatty acid desaturase 2 OS=Danio rerio GN=fads2 PE... [more]
FADS2_XENLA1.606e-2028.38Fatty acid desaturase 2 OS=Xenopus laevis GN=fads2... [more]
FADS2_BOVIN2.098e-2027.04Fatty acid desaturase 2 OS=Bos taurus GN=FADS2 PE=... [more]
FADS3_BOVIN2.740e-2028.24Fatty acid desaturase 3 OS=Bos taurus GN=FADS3 PE=... [more]
FADS1_HUMAN2.740e-2026.89Fatty acid desaturase 1 OS=Homo sapiens GN=FADS1 P... [more]
FAD2L_MOUSE2.740e-2030.67Fatty acid desaturase 2-like protein OS=Mus muscul... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_2_013G04_T7 Ruby Orange Developing Flower cDNA Library UCRCS04-UCR Citrus sinensis cDNA clone UCRCS04_013_T7_G04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV885281 ID=CV885281; Name=CV885281; organism=Citrus sinensis; type=EST; length=847bp
CGGAAATAAAATAGAGATCTCAAAACCGAAAGCAAACCAAATCATCACAT
CCCTTGCTATTTCACTTTGAGCAAAACTTGCGGGATTTTCTTGATTTGAT
TTTGCTTCCCAGGTGGCATAACCTATCTTCCCCCACAACACTTTCCAATT
TTTCAATCTGCTGCCTTAATGGAGGCCGAGAAAAAATACATAACGGCTGA
AGAGCTTAGAACCCACAACAAGGCAGAAGATTTGTGGATCTCTATACAGG
GGAAAGTTTACGACGTTACAGAATGGGCAAAACAACATCCTGGCGGCGAC
GTTCCGCTCTTGAATTTGGCTGGCCAAGATGTAACCGATGCGTTTATAGC
GTATCATCCAGGAACCGCTTGGCAATATCTTGATAAACTTTTTACAGGCT
ATTTTGTTCAAGATTTCGAAGTATCTGAAATCTCAAAAGATTACAGGAGA
CTGTACATTGAGTTTGCCAAACAGGGTATGTTTGAAAAGAAACAACACGT
GGCATCCTGTGCCCTTACATGTGTAGCATTGATGTTTGTTGTTGTGGTTT
ATGGCGTTTTGTGCTGTGAGAGCGTCTGGGCTCATTTGGGTTCAGGCATG
TTGTTGGGGTTTCTTTGGATTCAAAGTGCTTATGTTGGTCACGATTCCGG
GCATTATCAGGTTATGACAAGTCCTAAGTTTAACAAAATTGCTCAGCTTA
TTTCAGGAAATTGTTTAACAGGAATCAGCATTGCTTGGTGGAAGTGGACT
CACAATGCCCACCACATTGCTTGCAACAGCCTTGATTACGATCCAGATCT
TCAACACATCCCAGTTTTTGCGGTATCTACGCGTTTATTTAATTCAA
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