CX293823

Overview
NameCX293823
Unique NameCX293823
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length469
Libraries
Library NameType
FlavSen1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS6B_MANSE (26S protease regulatory subunit 6B OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.096e-16
Identity = 46/148 (31.08%), Postives = 82/148 (55.41%), Query Frame = 3
Query:   24 TEYRKKLLQHKEIESRVRSVKENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            T+Y+K     + +E +   +K+  R  KKE+   ++++K +QSV  +IG+ L  +D    IV +++G  Y V   S +D+E L     V L   +  ++  LP E D  +  +  ++  +V YS +GG+  Q +E+RE++ELPL + E
Sbjct:   39 TKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVE 186          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS6B_ENCCU (26S protease regulatory subunit 6B homolog OS=Encephalitozoon cuniculi GN=ECU08_1970 PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 3.576e-16
Identity = 47/146 (32.19%), Postives = 83/146 (56.85%), Query Frame = 3
Query:   30 YRKKLLQHKEIESRVRSVKENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            Y++   + + +E R   V++ +   K+E   + ++L  ++SV  I+G+ L P+D+   IV +++G  +VV   S VD+E L   T V L   +  I+  LP EVD  +  M   +  +V+Y  VGGL  Q +E++E++ELPL+  +
Sbjct:   13 YKQMERKLRLLEIREGYVRKEIETLKREERHSREELDRVKSVPLIMGQFLEPIDSSTAIVGSTAGSNFVVRILSTVDRELLKPNTTVALHRHSSAIVGVLPPEVDSTIPVMGESEKPSVTYGDVGGLDVQKQEIKETVELPLLQSD 158          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PSMR_METVS (Proteasome-activating nucleotidase OS=Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) GN=pan PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.670e-16
Identity = 48/145 (33.10%), Postives = 84/145 (57.93%), Query Frame = 3
Query:   57 EIESRVRSV--------KENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            E+ES+V  +        +EN++  KKE    + +L  L+    I+G VL  ++  + +VK+S+GP ++V     V+ + +  G RV L+  TL ++  LP+E D     M  E+  ++ +  +GGL++QIR+++E +ELPL NPE
Sbjct:   29 ELESKVLRLELKNKDITRENVQI-KKENEILKRELDKLRIPPLILGTVLDRVNERKAVVKSSTGPNFLVNLSQFVEPDDIVPGARVCLNQQTLAVVEVLPKEKDYRAMAMELEEKPDILFGDIGGLNNQIRDIKEVVELPLKNPE 172          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PSMR_AERPE (Proteasome-activating nucleotidase OS=Aeropyrum pernix GN=pan PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 4.670e-16
Identity = 47/135 (34.81%), Postives = 76/135 (56.30%), Query Frame = 3
Query:   60 IESRVRSVKENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNP 464
            ++ +VRS+ +   + +KE    ++++  L S   I   VL  +D+ R++VK+S+GP  +V   + VD   L  G  V L+    TI+  LP   DP+V  M  E+   V +  VGGL +QIRE+ E++ LP+ NP
Sbjct:   28 LKDKVRSLTKEKISLQKELEYYKNEITKLLSPPYIEAVVLEVIDDNRVVVKSSTGPNLIVNVAAGVDARSLKPGAIVALNNRGSTIVDVLPGRYDPLVKAMEVEERPKVFFKDVGGLEEQIREIYEAVVLPIKNP 162          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS8_YEAST (26S protease regulatory subunit 8 homolog OS=Saccharomyces cerevisiae GN=RPT6 PE=1 SV=4)

HSP 1 Score: 83.1889 bits (204), Expect = 6.099e-16
Identity = 45/145 (31.03%), Postives = 83/145 (57.24%), Query Frame = 3
Query:   54 KEIESRVRSVKENLRAAKKEFNKT-------EDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            +E E ++RS  EN+R  + + N         +D+L+ LQ  G  +GEV++ + +++++VK     +Y+V     ++ + L +  RV L   +  + + L  + DP+V  M+ E   + +Y  VGGL+ QI+E++E IELP+ +PE
Sbjct:   28 QETELKIRSKTENVRRLEAQRNALNDKVRFIKDELRLLQEPGSYVGEVIKIVSDKKVLVKVQPEGKYIVDVAKDINVKDLKASQRVCLRSDSYMLHKVLENKADPLVSLMMVEKVPDSTYDMVGGLTKQIKEIKEVIELPVKHPE 172          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS6B_SCHPO (26S protease regulatory subunit 6B homolog OS=Schizosaccharomyces pombe GN=rpt3 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 7.966e-16
Identity = 43/125 (34.40%), Postives = 75/125 (60.00%), Query Frame = 3
Query:   81 VKENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPL 455
            +KE+ ++ K+E  + ++++K +QSV  +IG+ L  +D    IV +++G  YVV   S +D+E L     V L   +  ++  LP E D  +  +  ++  +VSY+ VGGL  Q +E+RE++ELPL
Sbjct:   30 IKEDCKSLKRELIRAQEEVKRIQSVPLVIGQFLEAIDQNTAIVGSTTGSNYVVRILSTLDRELLKPSASVALQRHSNALVDILPPEADSSISMLRPDERPDVSYADVGGLDVQKQEVREAVELPL 154          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS4_ORYSJ (26S protease regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica GN=TBP2 PE=2 SV=2)

HSP 1 Score: 82.8037 bits (203), Expect = 7.966e-16
Identity = 45/136 (33.09%), Postives = 74/136 (54.41%), Query Frame = 3
Query:   60 IESRVRSVKENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            +E    + +E LR  + +  +    +  L+     +G +   +D    IV +S GP Y VG  S VDK++L  G  +++    L+++  L  EVDP+V  M  E     SY+ +GGL  QI+E++E++ELPL +PE
Sbjct:   82 MEEEFVAAQERLRPTEDKTEEDRSKVDDLRGTPMSVGSLEEIIDESHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPE 217          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS4B_ARATH (26S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana GN=RPT2B PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 7.966e-16
Identity = 48/142 (33.80%), Postives = 78/142 (54.93%), Query Frame = 3
Query:   42 LLQHKEIESRVRSVKENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            LL  +E  +    +K     A+++ +K +D    L+     +G +   +D    IV +S GP Y VG  S VDK++L  G  +++    L+++  L  EVDP+V  M  E     SY+ +GGL  QI+E++E++ELPL +PE
Sbjct:   75 LLMEEEFVANQERLKPQEEKAEEDRSKVDD----LRGTPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPE 212          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS4A_ARATH (26S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana GN=RPT2A PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 7.966e-16
Identity = 48/142 (33.80%), Postives = 78/142 (54.93%), Query Frame = 3
Query:   42 LLQHKEIESRVRSVKENLRAAKKEFNKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            LL  +E  +    +K     A+++ +K +D    L+     +G +   +D    IV +S GP Y VG  S VDK++L  G  +++    L+++  L  EVDP+V  M  E     SY+ +GGL  QI+E++E++ELPL +PE
Sbjct:   75 LLMEEEFVANQERLKPQEEKAEEDRSKVDD----LRGTPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPE 212          
BLAST of CX293823 vs. ExPASy Swiss-Prot
Match: PRS8_DICDI (26S protease regulatory subunit 8 OS=Dictyostelium discoideum GN=psmC5 PE=1 SV=2)

HSP 1 Score: 82.4185 bits (202), Expect = 1.040e-15
Identity = 49/145 (33.79%), Postives = 82/145 (56.55%), Query Frame = 3
Query:   54 KEIESRVRSVKENLR---AAKKEFNKT----EDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPE 467
            +E+E +V    ++LR   A + E N      +++L+ L + G  + EV++ +   +++VK +   ++VV     VD  KLT  TR  L   + T+ R LP ++DP+V  M  E   + +Y  VGGL  QI+E++E IELP+ +PE
Sbjct:   25 EELEIKVNEKAQDLRRLEAQRNELNNRVRMLKEELQLLTNPGSHVAEVVKLMGKNKVLVKVNPEGKFVVDIDPTVDIAKLTPSTRAALKHESYTLHRILPNKIDPLVSLMKVEKIPDSTYDMVGGLDKQIKEIKEVIELPIKHPE 169          
The following BLAST results are available for this feature:
BLAST of CX293823 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 84
Match NameE-valueIdentityDescription
PRS6B_MANSE2.096e-1631.0826S protease regulatory subunit 6B OS=Manduca sext... [more]
PRS6B_ENCCU3.576e-1632.1926S protease regulatory subunit 6B homolog OS=Ence... [more]
PSMR_METVS4.670e-1633.10Proteasome-activating nucleotidase OS=Methanococcu... [more]
PSMR_AERPE4.670e-1634.81Proteasome-activating nucleotidase OS=Aeropyrum pe... [more]
PRS8_YEAST6.099e-1631.0326S protease regulatory subunit 8 homolog OS=Sacch... [more]
PRS6B_SCHPO7.966e-1634.4026S protease regulatory subunit 6B homolog OS=Schi... [more]
PRS4_ORYSJ7.966e-1633.0926S protease regulatory subunit 4 homolog OS=Oryza... [more]
PRS4B_ARATH7.966e-1633.8026S proteasome regulatory subunit 4 homolog B OS=A... [more]
PRS4A_ARATH7.966e-1633.8026S proteasome regulatory subunit 4 homolog A OS=A... [more]
PRS8_DICDI1.040e-1533.7926S protease regulatory subunit 8 OS=Dictyostelium... [more]

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Properties
Property NameValue
Genbank descriptionC05001B03SK FlavSen1 Citrus clementina cDNA clone C05001B03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX293823 ID=CX293823; Name=CX293823; organism=Citrus clementina; type=EST; length=469bp
CCGTTAGACGCCGTAATGCCATGACGGAGTACCGTAAGAAGCTACTCCAG
CACAAAGAAATCGAATCTAGAGTTCGATCTGTTAAGGAGAATTTGAGAGC
TGCAAAGAAGGAGTTCAACAAAACAGAAGATGATTTGAAGTCTCTTCAAA
GTGTTGGGCAGATCATTGGAGAAGTTCTCAGACCTCTTGACAATGAACGC
TTGATTGTTAAAGCTAGCAGCGGGCCCCGTTATGTGGTTGGATGCCGCAG
CAAAGTGGATAAAGAGAAGTTGACTTCGGGAACAAGAGTTGTTCTTGATA
TGACAACGCTCACAATCATGCGAGCTCTTCCTCGTGAAGTTGATCCGGTT
GTATATAACATGTTGCATGAAGATCCGGGTAATGTTAGCTATTCGGCTGT
GGGTGGTTTATCTGATCAGATCAGAGAATTAAGGGAATCTATTGAGCTAC
CTTTGATGAATCCTGAGCT
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