CX296370

Overview
NameCX296370
Unique NameCX296370
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length671
Libraries
Library NameType
FerrChloL1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_MICAN (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Microcystis aeruginosa (strain NIES-843) GN=ispH PE=3 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 3.215e-49
Identity = 88/147 (59.86%), Postives = 115/147 (78.23%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D K F+  L +SDNYNR+GFGHKEE ++ M  EY SD+I++++EN +    G+V + LA+++GFCWGVERAV +AYE R+ FP+E+IWITNEIIHNP+VN+RL  MAV  IPVE G+K F VV  GDVV+LPAFGA+V EM  LN++
Sbjct:    2 DTKSFKRTLQQSDNYNRKGFGHKEEVMDAMTNEYQSDLIQEIRENNYRLQRGDVTIYLAQAFGFCWGVERAVAMAYETRQHFPQERIWITNEIIHNPSVNQRLRSMAVGFIPVENGQKDFTVVESGDVVILPAFGASVSEMQILNDK 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_CYAA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain ATCC 51142) GN=ispH PE=3 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.494e-46
Identity = 86/147 (58.50%), Postives = 114/147 (77.55%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D K F+ +L +S+NY+R+GFGH+ E +  MN EY S +I+K++EN +++  G+V +KLAE++GFCWGVERAV +AYE R+ FP+EKIWITNEIIHNP+VN+RL EM V  I V  G K F VV  GDVV+LPAFGA+V EM  LN++
Sbjct:    2 DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAEAFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDFSVVEAGDVVILPAFGASVTEMQLLNDK 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_SYNP2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=ispH PE=3 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 5.676e-46
Identity = 85/147 (57.82%), Postives = 110/147 (74.83%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D K F+ +L  S+NY R+GFGHK+E   +M  EY S +I+++++N +E T G+V + LAE++GFCWGVERAV +AYE RK FP EKIW+TNEIIHNP+VN RL+EM V  I V +G K F  V  GDVV+LPAFGA V+EM  LN+R
Sbjct:    2 DTKAFKRSLNHSENYYRQGFGHKDEVAGMMTSEYQSSLIQEIRDNNYELTRGDVTIYLAEAFGFCWGVERAVAMAYETRKHFPTEKIWVTNEIIHNPSVNNRLKEMNVHFIEVVDGDKDFSGVANGDVVILPAFGATVQEMQLLNDR 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_ACAM1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Acaryochloris marina (strain MBIC 11017) GN=ispH PE=3 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 3.679e-45
Identity = 80/148 (54.05%), Postives = 116/148 (78.38%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVE-EGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D K F+ +L +S+ Y+R+GFGH+EE   L+  EY S +I++++ NG+    G+V + LAE++GFCWGVERA+ +AYE R+QFP+E+IWITNEIIHNP+VNK L +M V+ IPV+ +G+K F VV + DVV+LPAFGA+V+EM  L+++
Sbjct:    2 DTKAFKRSLHKSEQYHRKGFGHEEEVTNLLQSEYNSKLIQQIRNNGYRLQQGDVTIHLAEAFGFCWGVERAIALAYETRRQFPQERIWITNEIIHNPSVNKNLRDMKVEFIPVQPDGQKDFTVVGQNDVVILPAFGASVQEMQLLHDK 149          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_THEEB (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elongatus (strain BP-1) GN=ispH PE=3 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 6.275e-45
Identity = 82/147 (55.78%), Postives = 111/147 (75.51%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D + F+ +L  S+NY+R+GFGH EE  + +  EY S +I++++ NG+ +  G+V ++LAE++GFCWGVERAV +AYE R  FP E+IWITNEIIHNP+VN+RL +MAV+ IPV  G K F  V  GDVV+LPAFGA+V+EM  LN R
Sbjct:    2 DTRAFKRSLHSSENYHRKGFGHGEEVNQQLQGEYQSSLIQQIRANGYRWQQGDVTIRLAEAFGFCWGVERAVALAYETRTHFPTERIWITNEIIHNPSVNERLRQMAVEFIPVVNGVKDFSGVRPGDVVILPAFGASVQEMQLLNER 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_SYNR3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain RCC307) GN=ispH PE=3 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 6.275e-45
Identity = 85/147 (57.82%), Postives = 106/147 (72.11%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D + F+ +L  S+ YNRRGFG  EE    + Q Y S++I+ ++ENG+E   G V ++LAE++GFCWGVERAV IAYE R+ +P E+IWITNEIIHNP+VN  L EM V  IPVEEG K F  V  GDVV+LPAFGA V+EM  LN R
Sbjct:    2 DTRAFKRSLHHSERYNRRGFGRAEEVAGSLEQAYQSELIQSIRENGYELREGRVTIRLAEAFGFCWGVERAVAIAYETRRHYPTERIWITNEIIHNPSVNAHLVEMNVLFIPVEEGVKDFSGVESGDVVILPAFGATVQEMQLLNER 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_CYAP7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7424) GN=ispH PE=3 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 1.398e-44
Identity = 77/147 (52.38%), Postives = 115/147 (78.23%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D K F+ +L +S+NY+R+GFG ++E + +MN EY S +I++++ N +  T G+V ++LAE++GFCWGVERAV ++YE R+ FP ++IW+TNE+IHNP+VN+RL +M V+ IPV E +K F VV + DVV+LPAFGA+V EM  LN++
Sbjct:    2 DTKAFKRSLQQSENYHRKGFGQEDEAMGVMNSEYQSSLIQEIRNNNYRLTQGDVTIRLAEAFGFCWGVERAVAMSYETRQHFPNQRIWLTNEVIHNPSVNQRLRDMKVEFIPVIEDQKDFSVVERDDVVILPAFGASVTEMQLLNDK 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_SYNP6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=ispH PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.826e-44
Identity = 80/147 (54.42%), Postives = 110/147 (74.83%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D K F+  L +SD YNR+GFG   +    +   Y SD+I+ L++NG+    G + ++LAE++GFCWGVERAV IAYE R+ FP+E+IWITNEIIHNP+VN+ L EM+V+ IP E G+K F VV++GDVV+LPAFGA+V+EM  L+ +
Sbjct:    2 DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAEAFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDFSVVDRGDVVILPAFGASVQEMQLLDEK 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_SYNE7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942) GN=ispH PE=3 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.826e-44
Identity = 80/147 (54.42%), Postives = 110/147 (74.83%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D K F+  L +SD YNR+GFG   +    +   Y SD+I+ L++NG+    G + ++LAE++GFCWGVERAV IAYE R+ FP+E+IWITNEIIHNP+VN+ L EM+V+ IP E G+K F VV++GDVV+LPAFGA+V+EM  L+ +
Sbjct:    2 DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAEAFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDFSVVDRGDVVILPAFGASVQEMQLLDEK 148          
BLAST of CX296370 vs. ExPASy Swiss-Prot
Match: ISPH_SYNPX (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain WH8102) GN=ispH PE=3 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 2.385e-44
Identity = 83/147 (56.46%), Postives = 106/147 (72.11%), Query Frame = 2
Query:  218 DAKLFRHNLTRSDNYNRRGFGHKEETLELMNQEYTSDIIKKLKENGFEYTWGNVKVKLAESYGFCWGVERAVQIAYEARKQFPEEKIWITNEIIHNPTVNKRLEEMAVQNIPVEEGKKQFDVVNKGDVVVLPAFGAAVEEMVTLNNR 658
            D   F+ +L  S+ YNRRGFG  EE  E + Q Y S +I  +++NG++ + G + V+LAE++GFCWGVERAV +AYE RK +P E++WITNEIIHNP+VN  L EM VQ IPVE+G K F  V  GDVV+LPAFGA V+EM  LN R
Sbjct:    2 DTHAFKRSLHHSERYNRRGFGRAEEVAESLEQAYQSGLIGTIRDNGYKLSHGRLTVRLAEAFGFCWGVERAVAMAYETRKHYPAERLWITNEIIHNPSVNDHLREMDVQFIPVEQGVKDFSGVTSGDVVILPAFGATVQEMQLLNER 148          
The following BLAST results are available for this feature:
BLAST of CX296370 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
ISPH_MICAN3.215e-4959.864-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_CYAA51.494e-4658.504-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNP25.676e-4657.824-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_ACAM13.679e-4554.054-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_THEEB6.275e-4555.784-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNR36.275e-4557.824-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_CYAP71.398e-4452.384-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNP61.826e-4454.424-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNE71.826e-4454.424-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]
ISPH_SYNPX2.385e-4456.464-hydroxy-3-methylbut-2-enyl diphosphate reductase... [more]

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Properties
Property NameValue
Genbank descriptionC05139G03SK FerrChloL1 Citrus clementina cDNA clone C05139G03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX296370 ID=CX296370; Name=CX296370; organism=Citrus clementina; type=EST; length=671bp
ACGAGGCTTTTGCGCCGTCTCTTTCTCGCTGAATCCGTGAATCTCCCTCA
TCATGGCGACGACTCTGCAATTCTGCCGTGTTTGCGCGCGAACTGACATA
GGTTCGCGCAGTAATAATCGCTCCGGAGCGCTGGCTCCGCCGCGCCGGAG
GCCTGTGATCGTCAGGTGCTCCGGTGAAAAATCGCCGAGCTCGGCGGCGG
CTATTGGTGCCGAATTTGACGCGAAACTGTTTCGGCATAACTTGACGAGA
AGTGATAATTACAATCGGAGAGGATTTGGCCACAAGGAAGAGACTTTGGA
GCTCATGAATCAGGAGTACACAAGTGATATTATAAAGAAATTGAAGGAGA
ATGGATTTGAGTATACTTGGGGAAATGTTAAGGTGAAGCTGGCCGAATCA
TATGGCTTTTGCTGGGGAGTTGAGCGTGCTGTGCAGATTGCTTATGAAGC
AAGAAAACAGTTTCCTGAGGAGAAGATCTGGATTACCAATGAAATCATAC
ACAATCCAACTGTTAATAAGCGGTTGGAGGAGATGGCAGTTCAAAATATA
CCTGTTGAGGAAGGAAAGAAGCAATTTGATGTGGTAAATAAGGGTGATGT
TGTGGTTTTGCCTGCATTTGGGGCTGCAGTTGAAGAAATGGTGACTCTGA
ATAACAGAATGTACAGATTGT
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