CX302557

Overview
NameCX302557
Unique NameCX302557
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length580
Libraries
Library NameType
SaltLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP1_PRUDU (Non-specific lipid-transfer protein 1 OS=Prunus dulcis PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 4.490e-25
Identity = 41/70 (58.57%), Postives = 56/70 (80.00%), Query Frame = 2
Query:   29 ILVTAPVTNAITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKRALRNNP 238
            ++V+ P+  AITCGQV+++LAPCIP++R GG  PP  CC+G+R++N  ARTTPDRQAACNCLK+   + P
Sbjct:   17 MVVSVPIAQAITCGQVSSNLAPCIPYVRGGGAVPPA-CCNGIRNVNNLARTTPDRQAACNCLKQLSASVP 85          

HSP 2 Score: 38.891 bits (89), Expect = 4.490e-25
Identity = 15/31 (48.39%), Postives = 21/31 (67.74%), Query Frame = 3
Query:  219 ELYGTIRGIKPNVAAGLPSQCGVRIPYKTQP 311
            +L  ++ G+ PN AA LP +CGV IPY+  P
Sbjct:   79 QLSASVPGVNPNNAAALPGKCGVNIPYQISP 109          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP_HELAN (Non-specific lipid-transfer protein OS=Helianthus annuus PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 2.915e-24
Identity = 43/65 (66.15%), Postives = 52/65 (80.00%), Query Frame = 2
Query:   29 ILVTAPVTNAITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKRA 223
            ++V AP T A++CGQV++SLAPCI +L  GG  PP  CCSGV+SLN AA+TTPDRQAAC CLK A
Sbjct:   16 MVVGAPYTEALSCGQVSSSLAPCISYLTKGGAVPPA-CCSGVKSLNSAAKTTPDRQAACGCLKSA 79          

HSP 2 Score: 35.039 bits (79), Expect = 2.915e-24
Identity = 14/29 (48.28%), Postives = 17/29 (58.62%), Query Frame = 3
Query:  225 YGTIRGIKPNVAAGLPSQCGVRIPYKTQP 311
            Y +I G+    AA  P +CGV IPYK  P
Sbjct:   80 YNSISGVNAGNAASFPGKCGVSIPYKISP 108          

HSP 3 Score: 23.8682 bits (50), Expect = 2.915e-24
Identity = 8/12 (66.67%), Postives = 12/12 (100.00%), Query Frame = 1
Query:  301 RLSPSTDCSRVR 336
            ++SPSTDCS+V+
Sbjct:  105 KISPSTDCSKVQ 116          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP_MALDO (Non-specific lipid-transfer protein OS=Malus domestica GN=MALD3 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.702e-24
Identity = 39/63 (61.90%), Postives = 50/63 (79.37%), Query Frame = 2
Query:   29 ILVTAPVTNAITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLK 217
            + +   V +AITCGQVT+SLAPCI ++R+GG  PP  CC+G+R++NG ARTT DRQ ACNCLK
Sbjct:   15 LCMAVSVAHAITCGQVTSSLAPCIGYVRSGGAVPPA-CCNGIRTINGLARTTADRQTACNCLK 76          

HSP 2 Score: 42.3578 bits (98), Expect = 3.702e-24
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = 3
Query:  222 LYGTIRGIKPNVAAGLPSQCGVRIPYK 302
            L G+I G+ PN AAGLP +CGV +PYK
Sbjct:   78 LAGSISGVNPNNAAGLPGKCGVNVPYK 104          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: LTP1_MORNI (Non-specific lipid-transfer protein 1 OS=Morus nigra PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 8.955e-23
Identity = 40/58 (68.97%), Postives = 46/58 (79.31%), Query Frame = 2
Query:   59 ITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKRALRN 232
            ITCGQV++SLAPCI +LR GG   P  CC+GVRSLN AA+TT DRQAACNCLK A  +
Sbjct:    1 ITCGQVSSSLAPCINYLRAGG-VVPANCCNGVRSLNNAAKTTADRQAACNCLKSAFNS 57          

HSP 2 Score: 40.817 bits (94), Expect = 8.955e-23
Identity = 15/29 (51.72%), Postives = 22/29 (75.86%), Query Frame = 3
Query:  225 YGTIRGIKPNVAAGLPSQCGVRIPYKTQP 311
            + +I+G+  N+AAGLP +CGV +PYK  P
Sbjct:   55 FNSIKGLNLNLAAGLPGKCGVSVPYKISP 83          

HSP 3 Score: 21.557 bits (44), Expect = 8.955e-23
Identity = 7/12 (58.33%), Postives = 10/12 (83.33%), Query Frame = 1
Query:  301 RLSPSTDCSRVR 336
            ++SPSTDC  V+
Sbjct:   80 KISPSTDCKSVK 91          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP1_PRUPE (Non-specific lipid-transfer protein 1 OS=Prunus persica PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.968e-22
Identity = 38/60 (63.33%), Postives = 49/60 (81.67%), Query Frame = 2
Query:   59 ITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKRALRNNP 238
            ITCGQV+++LAPCIP++R GG  PP  CC+G+R++N  ARTTPDRQAACNCLK+   + P
Sbjct:    1 ITCGQVSSALAPCIPYVRGGGAVPPA-CCNGIRNVNNLARTTPDRQAACNCLKQLSASVP 59          

HSP 2 Score: 38.1206 bits (87), Expect = 1.968e-22
Identity = 15/28 (53.57%), Postives = 20/28 (71.43%), Query Frame = 3
Query:  219 ELYGTIRGIKPNVAAGLPSQCGVRIPYK 302
            +L  ++ G+ PN AA LP +CGV IPYK
Sbjct:   53 QLSASVPGVNPNNAAALPGKCGVHIPYK 80          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP_PRUAV (Non-specific lipid-transfer protein OS=Prunus avium PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 9.330e-22
Identity = 36/70 (51.43%), Postives = 53/70 (75.71%), Query Frame = 2
Query:   29 ILVTAPVTNAITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKRALRNNP 238
            ++V+ P+  A+TCGQV+++LAPCI ++R GG  PP  CC+G+R++N  A+TT DRQ ACNCLK+   + P
Sbjct:   17 MVVSVPIAQALTCGQVSSNLAPCIAYVRGGGAVPPA-CCNGIRNINNLAKTTADRQTACNCLKQLSASVP 85          

HSP 2 Score: 36.965 bits (84), Expect = 9.330e-22
Identity = 14/31 (45.16%), Postives = 20/31 (64.52%), Query Frame = 3
Query:  219 ELYGTIRGIKPNVAAGLPSQCGVRIPYKTQP 311
            +L  ++ G+  N AA LP +CGV +PYK  P
Sbjct:   79 QLSASVPGVNANNAAALPGKCGVNVPYKISP 109          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP_PYRCO (Non-specific lipid-transfer protein OS=Pyrus communis PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.216e-21
Identity = 37/63 (58.73%), Postives = 51/63 (80.95%), Query Frame = 2
Query:   29 ILVTAPVTNAITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLK 217
            + +   V +AITC QV+A+LAPCI ++R+GG  PP  CC+G++++NG A+TTPDRQAACNCLK
Sbjct:   15 LCMAVSVAHAITCSQVSANLAPCINYVRSGGAVPPA-CCNGIKTINGLAKTTPDRQAACNCLK 76          

HSP 2 Score: 35.4242 bits (80), Expect = 1.216e-21
Identity = 13/27 (48.15%), Postives = 18/27 (66.67%), Query Frame = 3
Query:  222 LYGTIRGIKPNVAAGLPSQCGVRIPYK 302
            L G++ G+ P  A  LP +CGV +PYK
Sbjct:   78 LAGSVSGVNPGNAESLPGKCGVNVPYK 104          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP1_PRUAR (Non-specific lipid-transfer protein 1 OS=Prunus armeniaca PE=1 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 1.243e-21
Identity = 35/54 (64.81%), Postives = 45/54 (83.33%), Query Frame = 2
Query:   59 ITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKR 220
            ITCGQV++SLAPCI ++R GG  PP  CC+G+R++N  ARTTPDR+ ACNCLK+
Sbjct:    1 ITCGQVSSSLAPCIGYVRGGGAVPPA-CCNGIRNVNNLARTTPDRRTACNCLKQ 53          

HSP 2 Score: 41.2022 bits (95), Expect = 1.243e-21
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3
Query:  219 ELYGTIRGIKPNVAAGLPSQCGVRIPYK 302
            +L G+I G+ PN AA LP +CGV IPYK
Sbjct:   53 QLSGSISGVNPNNAAALPGKCGVNIPYK 80          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP1_TOBAC (Non-specific lipid-transfer protein 1 OS=Nicotiana tabacum GN=LTP1 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.506e-21
Identity = 37/65 (56.92%), Postives = 46/65 (70.77%), Query Frame = 2
Query:   29 ILVTAPVTNAITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKRA 223
            ++V AP   AITCGQVT++LAPC+ +LR  G  P   CC GV++L  +ARTT DRQ AC CLK A
Sbjct:   15 MVVAAPCAEAITCGQVTSNLAPCLAYLRNTG--PLGRCCGGVKALVNSARTTEDRQIACTCLKSA 77          

HSP 2 Score: 39.2762 bits (90), Expect = 5.506e-21
Identity = 18/28 (64.29%), Postives = 18/28 (64.29%), Query Frame = 3
Query:  228 GTIRGIKPNVAAGLPSQCGVRIPYKTQP 311
            G I GI    AAGLPS CGV IPYK  P
Sbjct:   79 GAISGINLGKAAGLPSTCGVNIPYKISP 106          

HSP 3 Score: 23.8682 bits (50), Expect = 5.506e-21
Identity = 8/12 (66.67%), Postives = 12/12 (100.00%), Query Frame = 1
Query:  301 RLSPSTDCSRVR 336
            ++SPSTDCS+V+
Sbjct:  103 KISPSTDCSKVQ 114          
BLAST of CX302557 vs. ExPASy Swiss-Prot
Match: NLTP1_SOLLC (Non-specific lipid-transfer protein 1 OS=Solanum lycopersicum GN=TSW12 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.616e-20
Identity = 37/65 (56.92%), Postives = 48/65 (73.85%), Query Frame = 2
Query:   29 ILVTAPVTNAITCGQVTASLAPCIPFLRTGGRFPPPPCCSGVRSLNGAARTTPDRQAACNCLKRA 223
            ++V AP   A+TCGQVTA LAPC+P+L+  GR P   CC GV++L G+A+TT DR+ AC CLK A
Sbjct:   15 MVVVAPHAEALTCGQVTAGLAPCLPYLQ--GRGPLGGCCGGVKNLLGSAKTTADRKTACTCLKSA 77          

HSP 2 Score: 35.8094 bits (81), Expect = 2.616e-20
Identity = 16/26 (61.54%), Postives = 18/26 (69.23%), Query Frame = 3
Query:  234 IRGIKPNVAAGLPSQCGVRIPYKTQP 311
            I+GI  N AAG+PS C V IPYK  P
Sbjct:   81 IKGIDLNKAAGIPSVCKVNIPYKISP 106          

HSP 3 Score: 22.7126 bits (47), Expect = 2.616e-20
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 1
Query:  301 RLSPSTDCSRVR 336
            ++SPSTDCS V+
Sbjct:  103 KISPSTDCSTVQ 114          
The following BLAST results are available for this feature:
BLAST of CX302557 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 70
Match NameE-valueIdentityDescription
NLTP1_PRUDU4.490e-2558.57Non-specific lipid-transfer protein 1 OS=Prunus du... [more]
NLTP_HELAN2.915e-2466.15Non-specific lipid-transfer protein OS=Helianthus ... [more]
NLTP_MALDO3.702e-2461.90Non-specific lipid-transfer protein OS=Malus domes... [more]
LTP1_MORNI8.955e-2368.97Non-specific lipid-transfer protein 1 OS=Morus nig... [more]
NLTP1_PRUPE1.968e-2263.33Non-specific lipid-transfer protein 1 OS=Prunus pe... [more]
NLTP_PRUAV9.330e-2251.43Non-specific lipid-transfer protein OS=Prunus aviu... [more]
NLTP_PYRCO1.216e-2158.73Non-specific lipid-transfer protein OS=Pyrus commu... [more]
NLTP1_PRUAR1.243e-2164.81Non-specific lipid-transfer protein 1 OS=Prunus ar... [more]
NLTP1_TOBAC5.506e-2156.92Non-specific lipid-transfer protein 1 OS=Nicotiana... [more]
NLTP1_SOLLC2.616e-2056.92Non-specific lipid-transfer protein 1 OS=Solanum l... [more]

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Properties
Property NameValue
Genbank descriptionC07012H03SK SaltLeaf1 Citrus clementina cDNA clone C07012H03, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX302557 ID=CX302557; Name=CX302557; organism=Citrus clementina; type=EST; length=580bp
CGGGANNNNNNNNNNNNNNNNNNNNNGCATTCTGGTGACTGCCCCGGTGA
CTAATGCCATAACATGCGGGCAGGTGACAGCCTCCTTGGCTCCATGCATC
CCTTTCTTGAGAACTGGAGGGCGTTTTCCTCCACCGCCATGCTGTAGCGG
CGTCAGGTCTCTCAACGGCGCCGCCAGAACCACCCCTGACCGCCAAGCTG
CATGCAACTGCTTGAAACGAGCTTTACGGAACAATCCGCGGCATCAAACC
TAATGTTGCCGCTGGCCTTCCTAGTCAATGTGGAGTCAGAATTCCTTACA
AGACTCAGCCCCAGCACCGACTGCTCTAGGGTCAGGTGAAGTTGAGTTTG
AGCTCTGTGACTTTTCCAAGTATGATTGACTTGAGAAGAATAAATAAATA
AAGAACATCTATGTTTAATTTCTTTTGTCCGAACGAGTCTCCACTTTGTA
TGAGTTTCACTCTCATGTCATGTTTGTATATTTGCTTTTTTATGTCATAT
TAAATATGTAAACGCGATCTTGTGTTTTGAGTGATTAATTATAGTTATTT
TGTGGTATTATCTTAAAAAAAAAAAAAAAA
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