Inhibition of quorum sensing (QS) in Yersinia enterocolitica by an orange extract rich in glycosylated flavanones

Publication Overview
TitleInhibition of quorum sensing (QS) in Yersinia enterocolitica by an orange extract rich in glycosylated flavanones
AuthorsTruchado P, Giménez-Bastida JA, Larrosa M, Castro-Ibáñez I, Espín JC, Tomás-Barberán FA, García-Conesa MT, Allende A
TypeJournal Article
Journal NameJournal of agricultural and food chemistry
Volume60
Issue36
Year2012
Page(s)8885-94
CitationTruchado P, Giménez-Bastida JA, Larrosa M, Castro-Ibáñez I, Espín JC, Tomás-Barberán FA, García-Conesa MT, Allende A. Inhibition of quorum sensing (QS) in Yersinia enterocolitica by an orange extract rich in glycosylated flavanones. Journal of agricultural and food chemistry. 2012 Sep 12; 60(36):8885-94.

Abstract

Flavanones, flavonoids abundant in Citrus , have been shown to interfere with quorum sensing (QS) and affect related physiological processes. We have investigated the QS-inhibitory effects of an orange extract enriched in O-glycosylated flavanones (mainly naringin, neohesperidin, and hesperidin). The QS-inhibitory capacity of this extract and its main flavanone components was first screened using the bacteriological monitoring system Chromobacterium violaceum . We next examined the ability of the orange extract and of some of the flavanones to (i) reduce the levels of the QS mediators produced by Y. enterocolitica using HPLC-MS/MS, (ii) inhibit biofilm formation, and (iii) inhibit swimming and swarming motility. Additionally, we evaluated changes in the expression of specific genes involved in the synthesis of the lactones (yenI, yenR) and in the flagellar regulon (flhDC, fleB, fliA) by RT-PCR. The results showed that the orange extract and its main flavanone components inhibited QS in C. violaceum, diminished the levels of lactones secreted by Y. enterocolitica to the media, and decreased QS-associated biofilm maturation without affecting bacterial growth. Among the tested compounds, naringin was found to inhibit swimming motility. Exposure to the orange extract and (or) to naringin was also found to be associated with induction of the transcription levels of yenR, flhDC, and fliA. This work shows the in vitro QS-inhibitory effects of an orange extract enriched in flavanones against a human enteropathogen at doses that can be achieved through the diet and suggests that consumption of these natural extracts may have a beneficial antipathogenic effect.

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Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1520-5118
eISSN1520-5118
Publication Date2012 Sep 12
Journal AbbreviationJ. Agric. Food Chem.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
Publication TypeResearch Support, Non-U.S. Gov't
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DatabaseAccession
PMID: PubMedPMID:22533445