FC923451

Overview
NameFC923451
Unique NameFC923451
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length675
Libraries
Library NameType
StrClemenNcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_EUCGU (Caffeic acid 3-O-methyltransferase OS=Eucalyptus gunnii GN=OMT PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.135e-29
Identity = 61/121 (50.41%), Postives = 78/121 (64.46%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAGE---LSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSVSG-----GERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWYII 472
            AMQLA   VLPM  +AAI+L + EI+AKAG    LS  E+AAQL  QN +AP+MLDR+ RLL S+ VL C++        ERLY L PV K+ V N+DG S+     +  DK+ MESWY +
Sbjct:   24 AMQLASASVLPMVLKAAIELDLLEIMAKAGPGAFLSPGEVAAQLPTQNPEAPVMLDRIFRLLASYSVLTCTLRNLPDGKVERLYGLAPVCKFLVKNEDGVSIAALNLMNQDKILMESWYYL 144          

HSP 2 Score: 43.1282 bits (100), Expect = 1.135e-29
Identity = 21/38 (55.26%), Postives = 27/38 (71.05%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNPGL*NL-SRGM 672
            LKDAV+EGGIPFN+ +GM  FEY   +P    + +RGM
Sbjct:  144 LKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKIFNRGM 181          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: OMT1_ARATH (Quercetin 3-O-methyltransferase 1 OS=Arabidopsis thaliana GN=OMT1 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 9.376e-29
Identity = 59/117 (50.43%), Postives = 77/117 (65.81%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAGELSAP-EIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSV-----SGGERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWY 466
            AMQLA   VLPMA ++A++L + EI+AK G   +P EIA++L  +N +AP+MLDR+LRLL S+ VL CS       G ER+Y L PV KY   N+DG S+     +  DKV MESWY
Sbjct:   23 AMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWY 139          

HSP 2 Score: 41.5874 bits (96), Expect = 9.376e-29
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDA+++GGIPFN+ +GM  FEY   +P
Sbjct:  141 LKDAILDGGIPFNKAYGMSAFEYHGTDP 168          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_CATRO (Caffeic acid 3-O-methyltransferase OS=Catharanthus roseus GN=COMT1 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 9.376e-29
Identity = 58/119 (48.74%), Postives = 78/119 (65.55%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAGE---LSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSVS-----GGERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWY 466
            AM+LA   VLPM  ++AI+L + E+I K+G    +S  E+AAQL  QN  AP+MLDR+LRLL S+ VL C++      G ERLY+L PV K+   N+DG S+   + +  DKV MESWY
Sbjct:   23 AMRLASASVLPMVLKSAIELDLLELIKKSGPGAYVSPSELAAQLPTQNPDAPVMLDRILRLLASYSVLNCTLKDLPDGGIERLYSLAPVCKFLTKNEDGVSMAALLLMNQDKVLMESWY 141          

HSP 2 Score: 42.743 bits (99), Expect = 9.376e-29
Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDAV+EGGIPFN+ +GM  FEY   +P
Sbjct:  143 LKDAVLEGGIPFNKAYGMTAFEYHGKDP 170          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_COFCA (Caffeic acid 3-O-methyltransferase OS=Coffea canephora PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.590e-28
Identity = 58/119 (48.74%), Postives = 77/119 (64.71%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAGE---LSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSVS-----GGERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWY 466
            AM LA   VLPM  ++AI+L + E+IAKAG    +S  E+AAQL   N +AP+MLDR+LRLL ++ VL+C ++     G ERLY L PV K+   N DG S+   + +  DKV MESWY
Sbjct:   10 AMSLASASVLPMVLKSAIELDLLELIAKAGPGAYVSPSELAAQLPTHNPEAPIMLDRILRLLATYSVLDCKLNNLADGGVERLYGLAPVCKFLTKNADGVSMAPLLLMNQDKVLMESWY 128          

HSP 2 Score: 41.5874 bits (96), Expect = 1.590e-28
Identity = 17/28 (60.71%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDAV++GGIPFN+ +GM  FEY   +P
Sbjct:  130 LKDAVLDGGIPFNKAYGMTAFEYHGTDP 157          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_POPKI (Caffeic acid 3-O-methyltransferase 1 OS=Populus kitakamiensis GN=HOMT1 PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 5.943e-28
Identity = 59/121 (48.76%), Postives = 75/121 (61.98%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAGE---LSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSVSG-----GERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWYII 472
            AMQLA   VLPM  + AI+L + EI+AKAG    LS  EIA+ L  +N  AP+MLDR+LRLL S+ +L CS+        ERLY L PV K+   N+DG S+     +  DKV MESWY +
Sbjct:   23 AMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSEIASHLPTKNPDAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPVCKFLTKNEDGVSVSPLCLMNQDKVLMESWYYL 143          

HSP 2 Score: 42.3578 bits (98), Expect = 5.943e-28
Identity = 17/28 (60.71%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDA++EGGIPFN+ +GM  FEY   +P
Sbjct:  143 LKDAILEGGIPFNKAYGMTAFEYHGTDP 170          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_MEDSA (Caffeic acid 3-O-methyltransferase OS=Medicago sativa PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.007e-27
Identity = 60/119 (50.42%), Postives = 76/119 (63.87%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAG---ELSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSVSGGE-----RLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWY 466
            AMQLA   VLPM  ++A++L + EIIAKAG   ++S  EIA+QL   N  AP+MLDRMLRLL  + +L CSV   +     RLY L  V+KY V N+DG S+     +  DKV MESWY
Sbjct:   23 AMQLASASVLPMILKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNPDAPVMLDRMLRLLACYIILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMNQDKVLMESWY 141          

HSP 2 Score: 41.5874 bits (96), Expect = 1.007e-27
Identity = 17/28 (60.71%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDAV++GGIPFN+ +GM  FEY   +P
Sbjct:  143 LKDAVLDGGIPFNKAYGMTAFEYHGTDP 170          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_POPTM (Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides GN=OMT1 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.311e-27
Identity = 59/121 (48.76%), Postives = 75/121 (61.98%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAGE---LSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSVSG-----GERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWYII 472
            AMQLA   VLPM  + AI+L + EI+AKAG    LS  EIA+ L  +N  AP+MLDR+LRLL S+ +L CS+        ERLY L PV K+   N+DG S+     +  DKV MESWY +
Sbjct:   23 AMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSEIASHLPTKNPDAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPVCKFLTKNEDGVSVSPLCLMNQDKVLMESWYYL 143          

HSP 2 Score: 41.2022 bits (95), Expect = 1.311e-27
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDA+++GGIPFN+ +GM  FEY   +P
Sbjct:  143 LKDAILDGGIPFNKAYGMTAFEYHGTDP 170          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT2_OCIBA (Caffeic acid 3-O-methyltransferase 2 OS=Ocimum basilicum GN=COMT2 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.707e-27
Identity = 57/123 (46.34%), Postives = 77/123 (62.60%), Query Frame = 2
Query:  122 SYSHAMQLAMGVVLPMATQAAIQLGVFEIIAKAGE---LSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSVS-----GGERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWY 466
            ++  AMQLA   VLPM  ++AI+L + E+I KAG    +S  E+AAQL   N +A +MLDR+LRLL S+ +LEC +      G +RLY L PV K+   N+DG S+     +  DKV MESWY
Sbjct:   17 NFLFAMQLASASVLPMVLKSAIELDLLELIKKAGAGAFVSPAELAAQLLTTNAEAHVMLDRILRLLTSYAILECRLKTLPDGGVQRLYGLAPVCKFLTKNEDGVSMAPLALMNQDKVLMESWY 139          

HSP 2 Score: 41.5874 bits (96), Expect = 1.707e-27
Identity = 17/28 (60.71%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDAV++GGIPFN+ +GM  FEY   +P
Sbjct:  141 LKDAVLDGGIPFNKAYGMTAFEYHGTDP 168          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_CLABR (Caffeic acid 3-O-methyltransferase OS=Clarkia breweri GN=COMT PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.222e-27
Identity = 61/124 (49.19%), Postives = 75/124 (60.48%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKA------GELSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSV-----SGGERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWYII 472
            AMQLA   VLPM  +AAI+L V EI+AK+        +S  EIAAQL   N  AP+MLDR+LRLL S+ V+ CS+        ERLY L PV K+   N+DG SL     +  DKV MESWY +
Sbjct:   25 AMQLASASVLPMVLKAAIELDVLEIMAKSIPHGSGAYISPAEIAAQLPTTNPDAPVMLDRVLRLLASYSVVTCSLRELPDGKVERLYGLAPVCKFLTKNEDGVSLAPLCLMNQDKVLMESWYYL 148          

HSP 2 Score: 41.2022 bits (95), Expect = 2.222e-27
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDA+++GGIPFN+ +GM  FEY   +P
Sbjct:  148 LKDAILDGGIPFNKAYGMTAFEYHGTDP 175          
BLAST of FC923451 vs. ExPASy Swiss-Prot
Match: COMT1_PRUDU (Caffeic acid 3-O-methyltransferase OS=Prunus dulcis GN=COMT1 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 2.893e-27
Identity = 58/119 (48.74%), Postives = 75/119 (63.03%), Query Frame = 2
Query:  134 AMQLAMGVVLPMATQAAIQLGVFEIIAKAGE---LSAPEIAAQLQAQNVKAPMMLDRMLRLLVSHRVLECSV-----SGGERLYALNPVSKYFVSNKDGASLGHFMALPLDKVFMESWY 466
            AMQLA   VLPM  +AAI+L + EI+AKAG    LS  +IA+QL  +N  AP+MLDRMLRLL S+ +L  S+        ERLY L PV K+   N++G S+     +  DKV +ESWY
Sbjct:   23 AMQLASASVLPMVLKAAIELDLLEIMAKAGPGVFLSPTDIASQLPTKNPDAPVMLDRMLRLLASYSILTYSLRTLADGKVERLYGLGPVCKFLTKNEEGVSIAPLCLMNQDKVLLESWY 141          

HSP 2 Score: 42.743 bits (99), Expect = 2.893e-27
Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 1
Query:  562 LKDAVMEGGIPFNRVHGMHIFEYASGNP 645
            LKDAV+EGGIPFN+ +GM  FEY   +P
Sbjct:  143 LKDAVLEGGIPFNKAYGMTAFEYHGTDP 170          
The following BLAST results are available for this feature:
BLAST of FC923451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 26
Match NameE-valueIdentityDescription
COMT1_EUCGU1.135e-2950.41Caffeic acid 3-O-methyltransferase OS=Eucalyptus g... [more]
OMT1_ARATH9.376e-2950.43Quercetin 3-O-methyltransferase 1 OS=Arabidopsis t... [more]
COMT1_CATRO9.376e-2948.74Caffeic acid 3-O-methyltransferase OS=Catharanthus... [more]
COMT1_COFCA1.590e-2848.74Caffeic acid 3-O-methyltransferase OS=Coffea canep... [more]
COMT1_POPKI5.943e-2848.76Caffeic acid 3-O-methyltransferase 1 OS=Populus ki... [more]
COMT1_MEDSA1.007e-2750.42Caffeic acid 3-O-methyltransferase OS=Medicago sat... [more]
COMT1_POPTM1.311e-2748.76Caffeic acid 3-O-methyltransferase 1 OS=Populus tr... [more]
COMT2_OCIBA1.707e-2746.34Caffeic acid 3-O-methyltransferase 2 OS=Ocimum bas... [more]
COMT1_CLABR2.222e-2749.19Caffeic acid 3-O-methyltransferase OS=Clarkia brew... [more]
COMT1_PRUDU2.893e-2748.74Caffeic acid 3-O-methyltransferase OS=Prunus dulci... [more]

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Properties
Property NameValue
Genbank descriptionC31705B10EF StrClemenN Citrus clementina cDNA clone C31705B10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>FC923451 ID=FC923451; Name=FC923451; organism=Citrus clementina; type=EST; length=675bp
GATGCCAAGTAAAATAAAAGCAATAGTAGTCAACCAAAAATAAAGCAACG
AAACAATGGGTTCTTTATCGGAATATCAAAAACTCGCTCAGAAAAAACAT
GAAGAAGAAGAAGAAGAAGAGAGCTATTCACATGCCATGCAGCTAGCAAT
GGGCGTAGTGCTGCCCATGGCAACCCAGGCAGCTATTCAGCTTGGCGTTT
TTGAAATCATAGCCAAAGCTGGGGAGCTCTCGGCTCCAGAGATTGCAGCG
CAGTTACAAGCGCAAAACGTAAAAGCACCAATGATGCTAGACAGAATGCT
TAGGCTTCTGGTCAGCCACCGTGTGCTTGAGTGCTCTGTTTCTGGTGGCG
AGCGGCTGTATGCTCTCAACCCTGTGTCCAAATATTTTGTTAGTAATAAA
GATGGAGCCTCGTTGGGTCACTTTATGGCCTTGCCTCTCGACAAGGTTTT
CATGGAGAGCTGGTACATTATTATTCTCTCTTTTTTTTTTTTTTTCCCCT
TTCGGGTCAAATCTATATAGTAGTCAATTTAAGTAATTTTAAAAATGCAT
GTAGGTTGGGATTGAAAGATGCAGTTATGGAGGGAGGAATACCATTCAAT
AGGGTGCATGGAATGCACATCTTTGAGTACGCTTCCGGAAACCCCGGTTT
ATGAAACTTATCACGAGGCATGTTC
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