Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment

Publication Overview
TitleTetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment
AuthorsAleza P, Froelicher Y, Schwarz S, Agustí M, Hernández M, Juárez J, Luro F, Morillon R, Navarro L, Ollitrault P
TypeJournal Article
Journal NameAnnals of botany
Volume108
Issue1
Year2011
Page(s)37-50
CitationAleza P, Froelicher Y, Schwarz S, Agustí M, Hernández M, Juárez J, Luro F, Morillon R, Navarro L, Ollitrault P. Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment. Annals of botany. 2011; 108(1):37-50.

Abstract

BACKGROUND AND AIMS: Polyploidy is a major component of plant evolution. The citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. The main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation. METHODS: Tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry and genotyped with 24 single sequence repeat (SSR) markers to analyse their genetic origin. Embryo rescue was used to grow all embryos contained in polyembryonic seeds of 'Tardivo di Ciaculli' mandarin, followed by characterization of the plantlets obtained by flow cytometry and SSR markers to accurately establish the rate of tetraploidization events and their potential tissue location. Inter-annual variations in tetraploid seedling rates were analysed for seven genotypes. Variation in tetraploid plantlet rates was analysed between different seedlings of the same genotype ('Carrizo' citrange; Citrus sinensis x Poncirus trifoliata) from seeds collected in different tropical, subtropical and Mediterranean countries. KEY RESULTS: Tetraploid plants were obtained for all the studied diploid genotypes, except for four mandarins. All tetraploid plants were identical to their diploid maternal line for SSR markers and were not cytochimeric. Significant genotypic and environmental effects were observed, as well as negative correlation between mean temperature during the flowering period and tetraploidy seedling rates. The higher frequencies (20 %) of tetraploids were observed for citranges cultivated in the Mediterranean area. CONCLUSIONS: Tetraploidization by chromosome doubling of nucellar cells are frequent events in apomictic citrus, and are affected by both genotypic and environmental factors. Colder conditions in marginal climatic areas appear to favour the expression of tetraploidization. Tetraploid genotypes arising from chromosome doubling of apomictic citrus are extensively being used as parents in breeding programmes to develop seedless triploid cultivars and have potential direct use as new rootstocks.
Features
This publication contains information about 13 features:
Feature NameUniquenameType
mCrCIR01C06mCrCIR01C06genetic_marker
CAC 15CAC 15genetic_marker
mCrCIR02D04bmCrCIR02D04bgenetic_marker
mCrCIR02G12mCrCIR02G12genetic_marker
mCrCIR03D12amCrCIR03D12agenetic_marker
Mest 123Mest 123genetic_marker
Mest 132Mest 132genetic_marker
Mest 15Mest 15genetic_marker
Mest 192Mest 192genetic_marker
Mest 56Mest 56genetic_marker
Mest 88Mest 88genetic_marker
TAA 1TAA 1genetic_marker
TAA 15TAA 15genetic_marker
Stocks
This publication contains information about 26 stocks:
Stock NameUniquenameType
MinneolaMinneolacultivar
MurcottMurcottcultivar
DuncanDuncancultivar
AnanaAnanacultivar
BeautyBeautycultivar
BoukhobzaBoukhobzacultivar
Cara CaraCara Caracultivar
FairchildFairchildcultivar
FremontFremontcultivar
KaraKaracultivar
KinnowKinnowcultivar
Mapo tangeloMapo tangelocultivar
MoroMorocultivar
Nadorcott tangorNadorcott tangorcultivar
Ortanique tangorOrtanique tangorcultivar
PagePagecultivar
Parson BrownParson Browncultivar
PineapplePineapplecultivar
SaltenitaSaltenitacultivar
SanguinelliSanguinellicultivar
ShamoutiShamouticultivar
SimetoSimetocultivar
Star RubyStar Rubycultivar
SunburstSunburstcultivar
Tardivo di CiaculliTardivo di Ciacullicultivar

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2011
Published Location|||
Language Abbreng
KeywordsCitroncirus webberi, Citrus, chromosomes, climatic zones, diploidy, environmental factors, evolution, flow cytometry, genes, genetic markers, genotype, genotype-environment interaction, mandarins, microsatellite repeats, parents, plantlets, rootstocks, seed collecting, seedless varieties, seedlings, seeds, temperature, tetraploidy
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4593577
PMID: PubMedPMID:21586529