CF417415 | Citrus Genome Database

Overview


Name	CF417415
Unique Name	CF417415
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	658

Libraries


Library Name	Type
Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig13335	contig	Csv1_Contig13335:744..1401.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 363.229 bits (931), Expect = 6.578e-100
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL
Sbjct:  411 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 358.992 bits (920), Expect = 1.241e-98
Identity = 171/174 (98.28%), Postives = 173/174 (99.43%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGR DPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITD+IHPAGWHEWDGNFALNTLFYGEHQN+GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL
Sbjct:  411 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 317.005 bits (811), Expect = 5.406e-86
Identity = 149/174 (85.63%), Postives = 161/174 (92.53%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            FFV CL+AGTVDFIFGN AAV Q+CDIHAR+P SGQKNMVTAQGRTDPNQNTGIVIQK RIGATSDL+PVQ SFPTYLGRPWKEYSRTVIMQSSITD+I PAGWHEW+GNFAL+TLFYGE+ N+GAGA TSGRVKWKG +VITS+TEAQA+TPG FIAG SWL STGFPFSLGL
Sbjct:  410 FFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME_DAUCA (Pectinesterase OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 314.694 bits (805), Expect = 2.683e-85
Identity = 147/174 (84.48%), Postives = 162/174 (93.10%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            FF+NC IAGTVDFIFGNAA VLQ+CDIHAR+P SGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDL+PVQ SFPTYLGRPWKEYSRTV+MQSSIT++I+PAGW  WDGNFAL+TL+YGE+QN+GAGA TSGRV WKGF+VITS+TEAQ FTPGSFIAG SWL +T FPFSLGL
Sbjct:  146 FFINCFIAGTVDFIFGNAAVVLQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVQSSFPTYLGRPWKEYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAATSGRVTWKGFKVITSSTEAQGFTPGSFIAGGSWLKATTFPFSLGL 319

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 294.278 bits (752), Expect = 3.753e-79
Identity = 138/174 (79.31%), Postives = 153/174 (87.93%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            FFV CLIAGTVDFIFGNAA VLQ+CDIHAR+PNSGQKNMVTAQGRTDPNQNTGIVIQK RIGATSDL+ V+GSFPTYLGRPWKEYS+TVIMQS+I+D+I P GW EW G FALNTL Y E+ N+GAGAGT+ RVKW+GF+VIT+A EAQ +T G FI G  WL STGFPFSLGL
Sbjct:  419 FFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 281.567 bits (719), Expect = 2.517e-75
Identity = 133/174 (76.44%), Postives = 147/174 (84.48%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            FFV C I GTVDFIFGNAAAVLQ+CDI+AR+PNSGQKNMVTAQGR+DPNQNTGIVIQ  RIG TSDL  V+G+FPTYLGRPWKEYSRTVIMQS I+D+I P GWHEW G+FAL+TL Y E+ N G GAGT+ RVKWKG++VITS TEAQ FT G FI G  WL STGFPFSL L
Sbjct:  414 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 234.187 bits (596), Expect = 4.609e-61
Identity = 113/174 (64.94%), Postives = 137/174 (78.74%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            F+ +C + GTVDFIFGNAAAVLQNC+I AR+P S + N +TAQGR+DPNQNTGI+I  SR+ A SDL+PV GS  TYLGRPW++YSRTV M++S+  LI P GW EWDGNFAL TLFY E QN+G GA TSGRV W GFRV+ SA+EA  FT G+F+AG SW+ S+  PF+ GL
Sbjct:  340 FYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPRS-KTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 1.255e-58
Identity = 105/174 (60.34%), Postives = 135/174 (77.59%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            F+  C I GTVDFIFGNAA VLQNC+I ARKP + + N +TAQGRTDPNQ+TGI+I   R+ A SDLKPVQ S  T+LGRPWK+YSRTV +++ +  LI+PAGW EW G+FALNTL+Y E+ N+G G+ T+ RVKW+G+ V+TS ++   FT G+FIAG+SWL +T  PF+ GL
Sbjct:  338 FYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME1_FICAW (Pectinesterase/pectinesterase inhibitor OS=Ficus awkeotsang PE=1 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 1.639e-58
Identity = 107/174 (61.49%), Postives = 131/174 (75.29%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            F+ +  I GTVDFIFGNAA V QNC++  RK   GQ+N +TAQGRTDPNQNTG  IQ   I A++DL+PV+ +F +YLGRPWKEYSRTV+M+S I+D+I PAGW EWD +FAL TLFYGE++N G G+GTS RVKW G+ VITS   A+ FT    I G SWLGSTG  ++ GL
Sbjct:  370 FYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMKGQENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAELIQGGSWLGSTGVDYTAGL 543

[Close]

BLAST of CF417415 vs. ExPASy Swiss-Prot
Match: PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 221.476 bits (563), Expect = 3.091e-57
Identity = 108/174 (62.07%), Postives = 125/174 (71.84%), Query Frame = 2

Query:    2 FFVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 523
            F+ +  + GTVDFIFGNAA V Q C + ARKP   Q+NMVTAQGRTDPNQ TG  IQ   I A+SDL+PV   FPTYLGRPWKEYSRTV+M+S +  LI+PAGW EWDG+FAL TL+YGE  N+G GAGTS RVKW G+ VIT   +A  FT    I G SWL STG  +  GL
Sbjct:  371 FYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRSTGVAYVDGL 544

The following BLAST results are available for this feature:

BLAST of CF417415 vs. ExPASy Swiss-Prot

BLAST of CF417415 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 84


Match Name	E-value	Identity	Description
PME1_CITSI	6.578e-100	100.00	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE...	[more]
PME3_CITSI	1.241e-98	98.28	Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1	[more]
PMEU1_SOLLC	5.406e-86	85.63	Pectinesterase/pectinesterase inhibitor U1 OS=Sola...	[more]
PME_DAUCA	2.683e-85	84.48	Pectinesterase OS=Daucus carota PE=1 SV=1	[more]
PME3_ARATH	3.753e-79	79.31	Pectinesterase/pectinesterase inhibitor 3 OS=Arabi...	[more]
PME2_ARATH	2.517e-75	76.44	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 P...	[more]
PME17_ARATH	4.609e-61	64.94	Probable pectinesterase/pectinesterase inhibitor 1...	[more]
PME2_CITSI	1.255e-58	60.34	Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE...	[more]
PME1_FICAW	1.639e-58	61.49	Pectinesterase/pectinesterase inhibitor OS=Ficus a...	[more]
PME1_SOLLC	3.091e-57	62.07	Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_115000-345 Citrus sinensis: Insect-damaged immature leaves/stems from field collected Valencia orange Citrus sinensis cDNA clone IDFL-004_H12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CF417415 ID=CF417415; Name=CF417415; organism=Citrus sinensis; type=EST; length=658bp
GTTCTTTGTGAACTGCTTAATTGCTGGCACGGTTGATTTCATTTTTGGTA
ATGCTGCAGCCGTGTTACAAAATTGTGACATCCATGCACGAAAGCCCAAT
TCCGGCCAAAAAAATATGGTCACAGCCCAAGGCAGGACTGACCCTAACCA
AAACACCGGCATTGTCATTCAAAAATCTAGGATTGGTGCCACCTCCGATT
TAAAACCGGTTCAGGGTAGTTTCCCGACGTACCTCGGCAGGCCCTGGAAG
GAGTACTCGAGGACGGTGATCATGCAGTCGTCGATTACTGACTTGATCCA
CCCTGCCGGGTGGCACGAGTGGGATGGTAACTTCGCGTTGAACACATTGT
TTTACGGAGAGCATCAGAACAGCGGAGCCGGTGCCGGAACTTCAGGGAGA
GTGAAATGGAAGGGATTTAGGGTTATTACAAGTGCTACCGAGGCTCAAGC
TTTTACTCCTGGAAGCTTCATTGCTGGTAGTAGCTGGCTGGGCTCCACTG
GTTTCCCATTCTCCCTTGGTTTGTAATATTCACTAGGAGTTTTAATTAAT
ATGTTTTGTATTAGTGGATCCATCGATCTCGGGTCTTTCAATTTGTAATA
TTTGATTGAGCGTGTCTTATTCGTGGCTTCGATCTCACAAATACTATTGT
GTGATTTA