EY728705 | Citrus Genome Database

Overview


Name	EY728705
Unique Name	EY728705
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	885

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY728705_ssr861	microsatellite	EY728705_ssr861:861..872.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G_PRUAV (Elongation factor 1-gamma OS=Prunus avium PE=2 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 1.261e-54
Identity = 101/147 (68.71%), Postives = 118/147 (80.27%), Query Frame = 2

Query:   26 LES*HPLLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAKKPTQ 466
            L++ +PL+GSSLID AHIEQWIDF SLEID NI+ WF PR G+AVYLP AEE             NTHLASNTYLVGH VTLADII+TCNL  GF++++ KSFTSEFPHVERYFWT+VN P F+K+LG++KQ ESVPPV SAKKP+Q
Sbjct:   77 LKADNPLIGSSLIDYAHIEQWIDFGSLEIDANIISWFRPRFGYAVYLPPAEEAAISALKRALGALNTHLASNTYLVGHFVTLADIIVTCNLFFGFTKLMIKSFTSEFPHVERYFWTLVNQPKFKKVLGDVKQTESVPPVPSAKKPSQ 223

HSP 2 Score: 22.3274 bits (46), Expect = 1.261e-54
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = 3

Query:    6 VRRYVARLKAD 38
            + RYVARLKAD
Sbjct:   70 IARYVARLKAD 80

HSP 3 Score: 97.4413 bits (241), Expect = 9.495e-32
Identity = 43/58 (74.14%), Postives = 50/58 (86.21%), Query Frame = 2

Query:  632 FQVKGLWLFRGQVIPSFIMYECYDMELYDWKKVDINDEAXKERVSQMIEDHEPFXGEA 805
            F+VKGLWLFRGQ IP F+M ECYDMELY+W KVD++DE  KERV+Q+IED EPF GEA
Sbjct:  357 FKVKGLWLFRGQEIPPFVMEECYDMELYNWTKVDLSDENQKERVNQVIEDQEPFEGEA 414

HSP 4 Score: 61.2326 bits (147), Expect = 9.495e-32
Identity = 28/29 (96.55%), Postives = 29/29 (100.00%), Query Frame = 3

Query:  552 VGGFLQRMDLARKYAFGKMLVIGSEPPFR 638
            VGGFLQRMDLARKYAFGKMLVIGSEPPF+
Sbjct:  330 VGGFLQRMDLARKYAFGKMLVIGSEPPFK 358

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G2_ORYSJ (Elongation factor 1-gamma 2 OS=Oryza sativa subsp. japonica GN=Os02g0220500 PE=2 SV=2)

HSP 1 Score: 199.134 bits (505), Expect = 2.724e-50
Identity = 94/141 (66.67%), Postives = 110/141 (78.01%), Query Frame = 2

Query:   38 HPLLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAKKP 460
            +PL GSSLI+ AHIEQW DFS+ E+D NI +W  PR+G A Y+  +EE             NTHLASNTYLVGHSVTLADI+MTCNL +GF+RI+TKSFTSEFPHVERYFWTMVN PNF+K+LG++KQAESVPPVQ    P
Sbjct:   81 NPLYGSSLIEYAHIEQWNDFSATEVDANIGKWLYPRLGIAPYVAVSEEAAIAALKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYMGFARIMTKSFTSEFPHVERYFWTMVNQPNFKKVLGDVKQAESVPPVQKKAPP 221

HSP 2 Score: 92.8189 bits (229), Expect = 6.489e-30
Identity = 43/58 (74.14%), Postives = 48/58 (82.76%), Query Frame = 2

Query:  632 FQVKGLWLFRGQVIPSFIMYECYDMELYDWKKVDINDEAXKERVSQMIEDHEPFXGEA 805
            F+VKGLWLFRG  IP F+M E YDMELY+W KVDI+DEA KERVS MIED EPF GE+
Sbjct:  353 FKVKGLWLFRGPEIPKFVMDEVYDMELYEWTKVDISDEAQKERVSAMIEDLEPFEGES 410

HSP 3 Score: 59.6918 bits (143), Expect = 6.489e-30
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  552 VGGFLQRMDLARKYAFGKMLVIGSEPPFR 638
            VGGFLQRMDL RKYAFGKMLVIGSEPPF+
Sbjct:  326 VGGFLQRMDLCRKYAFGKMLVIGSEPPFK 354

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G3_ORYSJ (Elongation factor 1-gamma 3 OS=Oryza sativa subsp. japonica GN=Os06g0571400 PE=2 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.766e-49
Identity = 94/139 (67.63%), Postives = 105/139 (75.54%), Query Frame = 2

Query:   44 LLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAKKP 460
            L GSSLID +HIEQW+DFS+ E+D NI RW  PR+GF  Y+P  EE             NTHLASNTYLVGHSVTLADI+MTCNL  GF RIL KSFTSEFPHVERYFWTMVN PNF+K++G+ KQAESVPPVQ    P
Sbjct:   83 LCGSSLIDYSHIEQWMDFSATEVDANIGRWLYPRLGFGPYVPVLEEFAITSLKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYYGFVRILIKSFTSEFPHVERYFWTMVNQPNFKKVIGDFKQAESVPPVQKKAAP 221

HSP 2 Score: 93.9745 bits (232), Expect = 1.432e-29
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 2

Query:  620 LGAT--FQVKGLWLFRGQVIPSFIMYECYDMELYDWKKVDINDEAXKERVSQMIEDHEPFXGE 802
            +G+T  F+VKGLWLFRGQ IP F+M E YDMELY+W KVD++DEA KERV+ MIED EPF GE
Sbjct:  345 IGSTPPFKVKGLWLFRGQDIPKFVMDEVYDMELYEWTKVDLSDEAQKERVNAMIEDQEPFEGE 407

HSP 3 Score: 57.3806 bits (137), Expect = 1.432e-29
Identity = 26/29 (89.66%), Postives = 27/29 (93.10%), Query Frame = 3

Query:  552 VGGFLQRMDLARKYAFGKMLVIGSEPPFR 638
            VGGFLQRMDL RKYAFGKMLVIGS PPF+
Sbjct:  324 VGGFLQRMDLCRKYAFGKMLVIGSTPPFK 352

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G1_ORYSJ (Elongation factor 1-gamma 1 OS=Oryza sativa subsp. japonica GN=Os02g0220600 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 3.934e-49
Identity = 91/139 (65.47%), Postives = 111/139 (79.86%), Query Frame = 2

Query:   29 ES*HPLLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQ 445
            +S +PL GSSLI+ AHIEQWIDFS+ E+D N  +W  PR+GFA Y+  +EE             NTHLASNTYLVGHSVTLADI+MTCNL +GF+RI+TK+FTSEFPHVERYFWTMVN PNF+K++G++KQA+SVP VQ
Sbjct:   78 KSDNPLYGSSLIEYAHIEQWIDFSATEVDANTGKWLFPRLGFAPYVAVSEEAAIAALKRSLGALNTHLASNTYLVGHSVTLADIVMTCNLYMGFARIMTKNFTSEFPHVERYFWTMVNQPNFKKVMGDVKQADSVPQVQ 216

HSP 2 Score: 93.9745 bits (232), Expect = 2.939e-30
Identity = 44/58 (75.86%), Postives = 48/58 (82.76%), Query Frame = 2

Query:  632 FQVKGLWLFRGQVIPSFIMYECYDMELYDWKKVDINDEAXKERVSQMIEDHEPFXGEA 805
            F+VKGLWLFRG  IP F+M E YDMELY+W KVDI+DEA KERVS MIED EPF GEA
Sbjct:  353 FKVKGLWLFRGPEIPKFVMDEVYDMELYEWTKVDISDEAQKERVSAMIEDLEPFEGEA 410

HSP 3 Score: 59.6918 bits (143), Expect = 2.939e-30
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  552 VGGFLQRMDLARKYAFGKMLVIGSEPPFR 638
            VGGFLQRMDL RKYAFGKMLVIGSEPPF+
Sbjct:  326 VGGFLQRMDLCRKYAFGKMLVIGSEPPFK 354

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G2_ARATH (Probable elongation factor 1-gamma 2 OS=Arabidopsis thaliana GN=At1g57720 PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 4.809e-47
Identity = 89/139 (64.03%), Postives = 105/139 (75.54%), Query Frame = 2

Query:   44 LLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAKKP 460
            L GSSLI+ AHIEQWIDFSSLEID N+L+WF PR+G+A +   AEE             NTHLASNT+LVGHSVTLADI+  CNL LGF+ ++TK FTS FPHVERYFWTMVN P F+K+LG+ KQ E+VPPV + K P
Sbjct:   83 LNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYAPFSAPAEEAAISALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTKKAP 221

HSP 2 Score: 97.0561 bits (240), Expect = 1.432e-29
Identity = 46/58 (79.31%), Postives = 49/58 (84.48%), Query Frame = 2

Query:  632 FQVKGLWLFRGQVIPSFIMYECYDMELYDWKKVDINDEAXKERVSQMIEDHEPFXGEA 805
            F+VKGLWLFRG  IP FIM E YDMELY+W KVDI+DEA KERVSQMIED EPF GEA
Sbjct:  348 FKVKGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEA 405

HSP 3 Score: 54.299 bits (129), Expect = 1.432e-29
Identity = 24/29 (82.76%), Postives = 27/29 (93.10%), Query Frame = 3

Query:  552 VGGFLQRMDLARKYAFGKMLVIGSEPPFR 638
            VGGFLQRMDLARKY+FGKML+ GSE PF+
Sbjct:  321 VGGFLQRMDLARKYSFGKMLICGSEGPFK 349

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G1_ARATH (Probable elongation factor 1-gamma 1 OS=Arabidopsis thaliana GN=At1g09640 PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 9.069e-46
Identity = 89/137 (64.96%), Postives = 102/137 (74.45%), Query Frame = 2

Query:   44 LLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAK 454
            L GSSLI+ A IEQWIDFSSLEI  +ILRWF PR+GF  Y   AEE             NTHL SNTYLVGHS+TLADII  CNL LGF+ ++TK FTSEFPHVERYFWT+VN PNF K+LG++KQ E+VPP+ S K
Sbjct:   83 LNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIASKK 219

HSP 2 Score: 97.0561 bits (240), Expect = 1.432e-29
Identity = 46/58 (79.31%), Postives = 49/58 (84.48%), Query Frame = 2

Query:  632 FQVKGLWLFRGQVIPSFIMYECYDMELYDWKKVDINDEAXKERVSQMIEDHEPFXGEA 805
            F+VKGLWLFRG  IP FIM E YDMELY+W KVDI+DEA KERVSQMIED EPF GEA
Sbjct:  349 FKVKGLWLFRGPEIPKFIMDEVYDMELYEWTKVDISDEAQKERVSQMIEDAEPFEGEA 406

HSP 3 Score: 54.299 bits (129), Expect = 1.432e-29
Identity = 24/29 (82.76%), Postives = 27/29 (93.10%), Query Frame = 3

Query:  552 VGGFLQRMDLARKYAFGKMLVIGSEPPFR 638
            VGGFLQRMDLARKY+FGKML+ GSE PF+
Sbjct:  322 VGGFLQRMDLARKYSFGKMLICGSEGPFK 350

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G_RAT (Elongation factor 1-gamma OS=Rattus norvegicus GN=Eef1g PE=2 SV=3)

HSP 1 Score: 90.1225 bits (222), Expect = 1.782e-17
Identity = 48/137 (35.04%), Postives = 69/137 (50.36%), Query Frame = 2

Query:   44 LLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAK 454
            L GS+   +A + QW+ F+  +I      W  P +G   +  QA E             +THL + T+LVG  VTLADI + C L   + ++L  SF   FP+  R+F T +N P FR ILGE+K  E +    + K
Sbjct:   84 LRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATENAKEEVKRILGLLDTHLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAILGEVKLCEKMAQFDAKK 220

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G_MOUSE (Elongation factor 1-gamma OS=Mus musculus GN=Eef1g PE=1 SV=3)

HSP 1 Score: 90.1225 bits (222), Expect = 1.782e-17
Identity = 48/137 (35.04%), Postives = 69/137 (50.36%), Query Frame = 2

Query:   44 LLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAK 454
            L GS+   +A + QW+ F+  +I      W  P +G   +  QA E             +THL + T+LVG  VTLADI + C L   + ++L  SF   FP+  R+F T +N P FR ILGE+K  E +    + K
Sbjct:   84 LRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHNKQATENAKEEVKRILGLLDTHLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAILGEVKLCEKMAQFDAKK 220

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1GA_XENLA (Elongation factor 1-gamma-A OS=Xenopus laevis GN=eef1g-A PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.782e-17
Identity = 47/137 (34.31%), Postives = 70/137 (51.09%), Query Frame = 2

Query:   44 LLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAK 454
            L G++ +  A + QW+ FS   I      W  P +G   Y  QA E             ++HL + T+LVG  +TLADI +TC+L   + ++L  SF   F +V R+F T VN P FR +LGE+K  + +    + K
Sbjct:   84 LRGTTRLHQAQVIQWVSFSDSHIVPPASAWVFPTLGIMQYNKQATEQAKEGIKTVLGVLDSHLQTRTFLVGERITLADITVTCSLLWLYKQVLEPSFRQPFGNVTRWFVTCVNQPEFRAVLGEVKLCDKMAQFDAKK 220

[Close]

BLAST of EY728705 vs. ExPASy Swiss-Prot
Match: EF1G_PIG (Elongation factor 1-gamma (Fragment) OS=Sus scrofa GN=EEF1G PE=2 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 2.328e-17
Identity = 47/137 (34.31%), Postives = 68/137 (49.64%), Query Frame = 2

Query:   44 LLGSSLIDSAHIEQWIDFSSLEIDTNILRWFIPRVGFAVYLPQAEEXXXXXXXXXXXXXNTHLASNTYLVGHSVTLADIIMTCNLTLGFSRILTKSFTSEFPHVERYFWTMVNIPNFRKILGEIKQAESVPPVQSAK 454
            L GS+   +A + QW+ F+  +I      W  P +G   Y  QA E             + HL + T+LVG  VTLADI + C L   + ++L  SF   FP+  R+F T +N P FR +LGE+K  E +    + K
Sbjct:   79 LRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHYNKQATENAKDEVRRVLGLLDAHLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKK 215

The following BLAST results are available for this feature:

BLAST of EY728705 vs. ExPASy Swiss-Prot

BLAST of EY728705 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19


Match Name	E-value	Identity	Description
EF1G_PRUAV	1.261e-54	68.71	Elongation factor 1-gamma OS=Prunus avium PE=2 SV=...	[more]
EF1G2_ORYSJ	2.724e-50	66.67	Elongation factor 1-gamma 2 OS=Oryza sativa subsp....	[more]
EF1G3_ORYSJ	1.766e-49	67.63	Elongation factor 1-gamma 3 OS=Oryza sativa subsp....	[more]
EF1G1_ORYSJ	3.934e-49	65.47	Elongation factor 1-gamma 1 OS=Oryza sativa subsp....	[more]
EF1G2_ARATH	4.809e-47	64.03	Probable elongation factor 1-gamma 2 OS=Arabidopsi...	[more]
EF1G1_ARATH	9.069e-46	64.96	Probable elongation factor 1-gamma 1 OS=Arabidopsi...	[more]
EF1G_RAT	1.782e-17	35.04	Elongation factor 1-gamma OS=Rattus norvegicus GN=...	[more]
EF1G_MOUSE	1.782e-17	35.04	Elongation factor 1-gamma OS=Mus musculus GN=Eef1g...	[more]
EF1GA_XENLA	1.782e-17	34.31	Elongation factor 1-gamma-A OS=Xenopus laevis GN=e...	[more]
EF1G_PIG	2.328e-17	34.31	Elongation factor 1-gamma (Fragment) OS=Sus scrofa...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-007-E05-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728705 ID=EY728705; Name=EY728705; organism=Citrus sinensis; type=EST; length=885bp
CACGCGTCCGACGATATGTTGCACGCTTGAAAGCTGACACCCACTGTTGG
GCTCGTCTTTAATTGATTCTGCTCATATTGAGCAGTGGATTGATTTCTCA
TCATTAGAAATTGACACCAATATTTTGCGTTGGTTTATACCAAGAGTTGG
TTTCGCGGTGTACCTTCCTCAGGCTGAGGAAGCTGCAATTGCTTCTTTGA
AGAGAGCTCTAGCTGCATTGAATACACATCTCGCTTCCAACACTTACTTA
GTTGGCCATTCAGTGACTTTGGCTGATATCATTATGACATGTAACTTGAC
GTTGGGATTCAGTCGGATCCTGACCAAGAGCTTTACATCAGAATTCCCTC
ACGTTGAGAGATACTTTTGGACAATGGTTAACATACCAAATTTCCGCAAG
ATATTGGGAGAGATAAAGCAAGCAGAATCTGTCCCTCCTGTTCAGTCAGC
GAAGAAGCCCACGCAGTCAAAAGAGCCTGCAAAACCCAAGGCCCAGGCTG
AGCCAAAGAAAGAGGCTGCAAAAGAAACAGCAAAACCTAAAGTTGAACAA
AGTGGGCGGATTCCTGCAGCGAATGGACTTGGCTCGTAAGTATGCTTTTG
GGAAGATGCTGGTTATTGGCTCGGAGCCACCTTTCAGGTAAAGGGTTTGT
GGCTTTTCCGTGGACAAGTGATTCCCTCATTTATAATGTACGAGTGTTAC
GACATGGAGTTGTATGATTGGAAGAAGGTGGACATCAATGATGAAGCTCA
NAAAGAGCGTGTAAGCCAGATGATCGAAGATCATGAACCCTTCGANGGTG
AGGCCTGTNAGATGCCAGTGCTTCAGTGAGATCACCAAATGGACCAACCG
GGGAATACTGGTGTGTGTGTGTATGCTGTTGCACC