QTL mapping of mandarin (Citrus reticulata) fruit characters using high-throughput SNP markers

Publication Overview
TitleQTL mapping of mandarin (Citrus reticulata) fruit characters using high-throughput SNP markers
AuthorsYu Y, Chen C, Gmitter F
TypeJournal Article
Journal NameTree Genetics & Genomes
Volume12
Issue77
Year2016
Page(s)1-16
CitationYu Y, Chen C, Gmitter F. QTL mapping of mandarin (Citrus reticulata) fruit characters using high-throughput SNP markers. Tree Genetics & Genomes. 2016; 12(77):1-16.

Abstract

Seedlessness, flavor, and color are top priorities for mandarin (Citrus reticulata Blanco) cultivar improvement. Given long juvenility, large tree size, and high breeding cost, marker-assisted selection (MAS) may be an expeditious and economical approach to these challenges. The objectives of this study were to construct high-density mandarin genetic maps and to identify single nucleotide polymorphism (SNP) markers associated with fruit quality traits. Two parental genetic maps were constructed from an F1 population derived from ‘Fortune’ × ‘Murcott’, two mandarin cultivars with distinct fruit characters, using a 1536-SNP Illumina GoldenGate assay. The map for ‘Fortune’ (FOR) consisted of 189 SNPs spanning 681.07 cM and for ‘Murcott’ (MUR) consisted of 106 SNPs spanning 395.25 cM. Alignment of the SNP sequences to the Clementine (Citrus clementina) genome showed highly conserved synteny between the genetic maps and the genome. A total of 48 fruit quality quantitative trait loci (QTLs) were identified, and ten of them stable over two or more samplings were considered as major QTLs. A cluster of QTLs for flavedo color space values L, a, b, and a/b and juice color space values a and a/b were detected in a single genomic region on linkage group 4. Two carotenoid biosynthetic pathway genes, pds1 and ccd4, were found within this QTL interval. Several SNPs were potentially useful in MAS for these fruit characteristics. QTLs were validated in 13 citrus selections, which may be useful in further validation and tentative MAS in mandarin fruit quality improvement.
Projects
This publication contains information about 1 projects:
Project NameDescription
Citrus-Fruit_Characters-Yu-2016
Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-Fortune/Murcott-Fortune-F1-1991
Citrus-Fortune/Murcott-Murcott-F1-1991
Stocks
This publication contains information about 14 stocks:
Stock NameUniquenameType
FortuneFortunecultivar
MurcottMurcottcultivar
Citrus-Fortune_x_Murcott-F1Citrus-Fortune_x_Murcott-F1population
GoutouGoutoucultivar
KingKingcultivar
MoroMorocultivar
NulesNulescultivar
OwariOwaricultivar
PimpledPimpledcultivar
PonkanPonkancultivar
RangpurRangpurcultivar
Citrus aurantiumCitrus aurantiumcultivar
SunkiSunkicultivar
ZhulaunZhulauncultivar
Properties
Additional details for this publication include:
Property NameValue
DOIdoi:10.1007/s11295-016-1034-7
URLhttps://link.springer.com/article/10.1007/s11295-016-1034-7
Year2016