EY702386

Overview
NameEY702386
Unique NameEY702386
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length649
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PLPP_RAT (Pyridoxal phosphate phosphatase OS=Rattus norvegicus GN=Pdxp PE=1 SV=2)

HSP 1 Score: 117.472 bits (293), Expect = 6.135e-26
Identity = 64/156 (41.03%), Postives = 98/156 (62.82%), Query Frame = 3
Query:    3 RVRGAVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVTT----QSTLQDPSNNIQPDYYTNQVSDILELLG 458
            RVR AV+VG D H ++ KL      +R+ P CL +AT+RD    LTD    PG G + AA+  ++ ++ +VVGKPS +M + +++ F +  +RM MVGDRL+TDILFG   G  T+L L+GV++    Q+ L    +++ P YY   ++D++E LG
Sbjct:  138 RVR-AVLVGYDEHFSFAKLTEACAHLRD-PDCLLVATDRDPWHPLTDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFQCITEDFSVDPARMLMVGDRLETDILFGHRCGMTTVLTLTGVSSLEEAQAYLAAGQHDLVPHYYVESIADLMEGLG 291          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PGP_CHICK (Phosphoglycolate phosphatase OS=Gallus gallus GN=PGP PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 6.782e-25
Identity = 60/154 (38.96%), Postives = 87/154 (56.49%), Query Frame = 3
Query:   15 AVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVTTQSTLQDPSNN-------IQPDYYTNQVSDILELL 455
            AV+VG D H +Y KL      +   P CL + TNRD    L      PG GC+V A+  + E+E ++VGKPS ++ + ++ +F I  +R  MVGDRLDTDIL G   G  TLL L+GV+T   ++    +       + PDYY + ++D+L  L
Sbjct:  157 AVLVGFDEHFSYAKLCQALRYLLRGPDCLLVGTNRDNRLPLEGGSAIPGTGCLVKAVETAAEREALIVGKPSRYIFDCVASEFDIDPARTIMVGDRLDTDILMGNTCGLTTLLTLTGVSTLEEVRGHQESDCPARQGLVPDYYVDSIADLLPAL 310          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PLPP_BOVIN (Pyridoxal phosphate phosphatase OS=Bos taurus GN=PDXP PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.973e-24
Identity = 61/153 (39.87%), Postives = 94/153 (61.44%), Query Frame = 3
Query:    3 RVRGAVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVTT----QSTLQDPSNNIQPDYYTNQVSDILE 449
            RVR AV+VG D H ++ KL      +R+ P CL +AT+RD    L+D    PG G + AA+  ++ ++ +VVGKPS +M E +++ F +   R  MVGDRL+TDILFG   G  T+L L+GV++    Q+ L    +++ P YY   ++D++E
Sbjct:  142 RVR-AVLVGYDEHFSFAKLSEACAHLRD-PDCLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFECITEHFSVDPGRTLMVGDRLETDILFGHRCGMTTVLTLTGVSSLEEAQAYLAAGQHDLVPHYYVESIADLME 292          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PLPP_HUMAN (Pyridoxal phosphate phosphatase OS=Homo sapiens GN=PDXP PE=1 SV=2)

HSP 1 Score: 112.079 bits (279), Expect = 2.577e-24
Identity = 62/153 (40.52%), Postives = 94/153 (61.44%), Query Frame = 3
Query:    3 RVRGAVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVT----TQSTLQDPSNNIQPDYYTNQVSDILE 449
            RVR AV+VG D H ++ KL+     +R+ P CL +AT+RD    L+D    PG G + AA+  ++ ++ +VVGKPS +M E +++ F I  +R  MVGDRL+TDILFG   G  T+L L+GV+     Q+ L    +++ P YY   ++D+ E
Sbjct:  142 RVR-AVLVGYDEHFSFAKLREACAHLRD-PECLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFECITENFSIDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSRLEEAQAYLAAGQHDLVPHYYVESIADLTE 292          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PGP_HUMAN (Phosphoglycolate phosphatase OS=Homo sapiens GN=PGP PE=1 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 4.396e-24
Identity = 63/157 (40.13%), Postives = 92/157 (58.60%), Query Frame = 3
Query:   15 AVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVTTQSTLQDPSNN----------IQPDYYTNQVSDILELL 455
            AVVVG DPH +Y KL      +++ PGCL + TN D    L + +   G GC+V A+  + +++  ++GKPS F+ + +S+++ I   R  MVGDRLDTDIL G   G KT+L L+GV   STL D  NN          + PD+Y + ++D+L  L
Sbjct:  167 AVVVGFDPHFSYMKLTKALRYLQQ-PGCLLVGTNMDNRLPLENGRFIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGINPERTVMVGDRLDTDILLGATCGLKTILTLTGV---STLGDVKNNQESDCVSKKKMVPDFYVDSIADLLPAL 319          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PLPP_MOUSE (Pyridoxal phosphate phosphatase OS=Mus musculus GN=Pdxp PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.083e-22
Identity = 58/153 (37.91%), Postives = 93/153 (60.78%), Query Frame = 3
Query:    3 RVRGAVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVTT----QSTLQDPSNNIQPDYYTNQVSDILE 449
            RVR AV+VG D   ++ +L      +R+ P CL +AT+RD    L+D    PG G + AA+  ++ ++ +VVGKPS +M + +++ F +  +R  MVGDRL+TDILFG   G  T+L L+GV++    Q+ L     ++ P YY   ++D++E
Sbjct:  138 RVR-AVLVGYDEQFSFSRLTEACAHLRD-PDCLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKPSPYMFQCITEDFSVDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSSLEEAQAYLTAGQRDLVPHYYVESIADLME 288          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PGP_BOVIN (Phosphoglycolate phosphatase OS=Bos taurus GN=PGP PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.414e-22
Identity = 60/157 (38.22%), Postives = 92/157 (58.60%), Query Frame = 3
Query:   15 AVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVTTQSTLQDPSNN----------IQPDYYTNQVSDILELL 455
            AVVVG DPH +Y KL      +++ P CL + TN D    L + +   G GC+V A+  + +++  ++GKPS F+ + +S+++ I   R  MVGDRLDTDIL G   G KT+L L+GV   S+L+D  +N          + PD+Y + ++D+L  L
Sbjct:  167 AVVVGFDPHFSYMKLTKAVRYLQQ-PDCLLVGTNMDNRLPLENGRFIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGIHPERTVMVGDRLDTDILLGVTCGLKTILTLTGV---SSLRDVKSNQESDCMAKKKMVPDFYVDSIADLLPAL 319          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PGP_MOUSE (Phosphoglycolate phosphatase OS=Mus musculus GN=Pgp PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.151e-22
Identity = 59/157 (37.58%), Postives = 91/157 (57.96%), Query Frame = 3
Query:   15 AVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGCKTLLVLSGVTTQSTLQDPSNN----------IQPDYYTNQVSDILELL 455
            AVVVG DPH +Y KL      +++ P CL + TN D    L + +   G GC+V A+  + +++  ++GKPS F+ + +S+++ I   R  MVGDRLDTDIL G     KT+L L+GV   S+L+D  +N          + PD+Y + ++D+L  L
Sbjct:  167 AVVVGFDPHFSYMKLTKAVRYLQQ-PDCLLVGTNMDNRLPLENGRFIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGINPERTVMVGDRLDTDILLGSTCSLKTILTLTGV---SSLEDVKSNQESDCMFKKKMVPDFYVDSIADLLPAL 319          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PNPP_SCHPO (4-nitrophenylphosphatase OS=Schizosaccharomyces pombe GN=pho2 PE=2 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 3.986e-17
Identity = 49/147 (33.33%), Postives = 82/147 (55.78%), Query Frame = 3
Query:   12 GAVVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGC-KTLLVLSGVTTQSTLQDPSNNIQPDYYTNQVSDILE 449
            GAV+ G+D H+ Y K       +++ P C F+ TN+D+    T+ +  PG+G +   +  ST ++P ++GKP   MME +         + C VGDRL+TDI F +N+    +LLVL+GV+ +  + +    + PDYY   ++ + E
Sbjct:  152 GAVLCGMDMHVTYLKYCMAFQYLQD-PNCAFLLTNQDST-FPTNGKFLPGSGAISYPLIFSTGRQPKILGKPYDEMMEAIIANVNFDRKKACFVGDRLNTDIQFAKNSNLGGSLLVLTGVSKEEEILEKDAPVVPDYYVESLAKLAE 296          
BLAST of EY702386 vs. ExPASy Swiss-Prot
Match: PNPP_YEAST (4-nitrophenylphosphatase OS=Saccharomyces cerevisiae GN=PHO13 PE=1 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 3.374e-16
Identity = 52/147 (35.37%), Postives = 81/147 (55.10%), Query Frame = 3
Query:   18 VVVGLDPHINYYKLQYGTLCIRENPGCLFIATNRDAVGHLTDLQEWPGAGCMVAAMCASTEKEPIVVGKPSTFMMEILSKKFQIASSRMCMVGDRLDTDILFGQNAGC-KTLLVLSGVTTQSTLQDPSNNI-QPDYYTNQVSDILEL 452
            V+ GLD  +NY++L   TL   +     F+ TN D+         +PGAG M+ ++  S+ + P   GKP+  M+  +   F +  S+ CMVGDRL+TD+ FG   G   TLLVLSG+ T+      S++  +P +Y +++ DI  L
Sbjct:  163 VIAGLDTKVNYHRLAV-TLQYLQKDSVHFVGTNVDSTFPQKGYT-FPGAGSMIESLAFSSNRRPSYCGKPNQNMLNSIISAFNLDRSKCCMVGDRLNTDMKFGVEGGLGGTLLVLSGIETEERALKISHDYPRPKFYIDKLGDIYTL 307          
The following BLAST results are available for this feature:
BLAST of EY702386 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 24
Match NameE-valueIdentityDescription
PLPP_RAT6.135e-2641.03Pyridoxal phosphate phosphatase OS=Rattus norvegic... [more]
PGP_CHICK6.782e-2538.96Phosphoglycolate phosphatase OS=Gallus gallus GN=P... [more]
PLPP_BOVIN1.973e-2439.87Pyridoxal phosphate phosphatase OS=Bos taurus GN=P... [more]
PLPP_HUMAN2.577e-2440.52Pyridoxal phosphate phosphatase OS=Homo sapiens GN... [more]
PGP_HUMAN4.396e-2440.13Phosphoglycolate phosphatase OS=Homo sapiens GN=PG... [more]
PLPP_MOUSE1.083e-2237.91Pyridoxal phosphate phosphatase OS=Mus musculus GN... [more]
PGP_BOVIN1.414e-2238.22Phosphoglycolate phosphatase OS=Bos taurus GN=PGP ... [more]
PGP_MOUSE3.151e-2237.58Phosphoglycolate phosphatase OS=Mus musculus GN=Pg... [more]
PNPP_SCHPO3.986e-1733.334-nitrophenylphosphatase OS=Schizosaccharomyces po... [more]
PNPP_YEAST3.374e-1635.374-nitrophenylphosphatase OS=Saccharomyces cerevisi... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-006-D08-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY702386 ID=EY702386; Name=EY702386; organism=Citrus sinensis; type=EST; length=649bp
CCCGCGTCCGTGGAGCAGTTGTGGTTGGATTAGATCCGCATATTAACTAT
TACAAGCTTCAGTATGGAACCCTTTGCATCCGTGAGAATCCTGGCTGCCT
TTTTATTGCTACCAATCGTGATGCAGTGGGGCATTTGACTGATTTACAAG
AGTGGCCTGGTGCCGGGTGTATGGTTGCAGCCATGTGTGCATCAACTGAA
AAAGAGCCTATTGTAGTTGGAAAGCCATCAACGTTCATGATGGAAATTTT
GTCAAAGAAATTTCAGATTGCTTCCTCCAGAATGTGTATGGTGGGTGATA
GATTAGATACTGATATTTTGTTTGGACAAAATGCTGGCTGCAAAACACTC
CTTGTTCTTTCAGGTGTGACAACTCAATCCACCCTCCAAGATCCCTCAAA
CAACATCCAACCAGATTATTACACAAATCAGGTGTCTGACATTCTTGAAT
TATTAGGACAGTAACCTTGCGATTACTGCTTCAATACTCACTTATTTGAG
GCCTGTTTGCTGGTTGCCCCATGCAGATCTTATGTATGCCCATAGAATGC
TGATTGTTTGTATTGAACTCGCTTATATTGAAACATCAAAAAGCAATTCG
CGCTTAAAACTGCAACATTGCTTTTGCTTTTAAAAAAAAAAAAAAAAAA
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