DR406092

Overview
NameDR406092
Unique NameDR406092
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length735
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13132 contig Csv1_Contig13132:223..957. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: FACE1_ARATH (CAAX prenyl protease 1 homolog OS=Arabidopsis thaliana GN=FACE1 PE=1 SV=1)

HSP 1 Score: 451.44 bits (1160), Expect = 2.243e-126
Identity = 223/244 (91.39%), Postives = 231/244 (94.67%), Query Frame = 3
Query:    3 GPYLAIYLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAIDEPDK 734
            GPYLAIYLWAFMF+LSLVMMT+YPVLIAPLFNKFTPLP+G+LREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKN +EIVAVIAHELGHWKLNHT YSFIAVQ+L  LQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIP+QHLVSFGLNLVSR+FEFQADAFA KLGYA  LR  LVKLQEENLSAMNTDP YSAYHYSHPPLVERL AID  DK
Sbjct:  178 GPYLAIYLWAFMFILSLVMMTIYPVLIAPLFNKFTPLPDGDLREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNEDEIVAVIAHELGHWKLNHTTYSFIAVQILAFLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPLQHLVSFGLNLVSRAFEFQADAFAVKLGYAKDLRPALVKLQEENLSAMNTDPLYSAYHYSHPPLVERLRAIDGEDK 421          
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: FACE1_ORYSJ (CAAX prenyl protease 1 homolog OS=Oryza sativa subsp. japonica GN=FACE1 PE=2 SV=1)

HSP 1 Score: 430.254 bits (1105), Expect = 5.351e-120
Identity = 206/243 (84.77%), Postives = 226/243 (93.00%), Query Frame = 3
Query:    3 GPYLAIYLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGYTLVRNSTDLFRSFGFDTQPVLIGLIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAIDEPD 731
            GPYLAIYLW FMF LSLVMMT+YP++IAPLFNKFTPLPEG LREKIEKLA+SL FPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQC + +EIV+VIAHELGHWKLNHT+YSF+AVQ+L  LQFGGYTLVRNS DLF SFGF+ QPV+IGLIIFQHT+IP+QHL+SF LNLVSR+FEFQADAFAK LGYA  LRA LVKLQEENLSAMNTDPWYSAYHYSHPPLVERL+A+++ D
Sbjct:  178 GPYLAIYLWGFMFALSLVMMTIYPIVIAPLFNKFTPLPEGVLREKIEKLAASLSFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCSSEDEIVSVIAHELGHWKLNHTVYSFVAVQLLMFLQFGGYTLVRNSKDLFESFGFEDQPVIIGLIIFQHTIIPVQHLLSFCLNLVSRAFEFQADAFAKNLGYAPQLRAALVKLQEENLSAMNTDPWYSAYHYSHPPLVERLSALEDAD 420          
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: FACE1_HUMAN (CAAX prenyl protease 1 homolog OS=Homo sapiens GN=ZMPSTE24 PE=1 SV=2)

HSP 1 Score: 250.366 bits (638), Expect = 7.590e-66
Identity = 137/278 (49.28%), Postives = 180/278 (64.75%), Query Frame = 3
Query:    3 GPYLAIYLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQ-------------------------------------CKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGYTLVRNSTDLFRSFGF-DTQPVLIG-LIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAI 719
            G Y  IY W F  V+SLV++T+Y   IAPLF+KFTPLPEG+L+E+IE +A S+ FPL K++VV+GS RSSHSNAY YGFFKNKRIVL+DTL+++                                     CKN EE++AV+ HELGHWKL HT+ + I  Q+ + L F  + ++    +LF +FGF D+QP LIG LIIFQ    P   ++SF L ++SR FEFQADAFAKKLG A  L + L+KL ++NL    +D  +S +HYSHPPL+ERL A+
Sbjct:  193 GDYFFIYAWLFTLVVSLVLVTIYADYIAPLFDKFTPLPEGKLKEEIEVMAKSIDFPLTKVYVVEGSKRSSHSNAYFYGFFKNKRIVLFDTLLEEYSVLNKDIQEDSGMEPRNEEEGNSEEIKAKVKNKKQGCKN-EEVLAVLGHELGHWKLGHTVKNIIISQMNSFLCFFLFAVLIGRKELFAAFGFYDSQPTLIGLLIIFQFIFSPYNEVLSFCLTVLSRRFEFQADAFAKKLGKAKDLYSALIKLNKDNLGFPVSDWLFSMWHYSHPPLLERLQAL 469          
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: FACE1_MOUSE (CAAX prenyl protease 1 homolog OS=Mus musculus GN=Zmpste24 PE=1 SV=2)

HSP 1 Score: 249.595 bits (636), Expect = 1.295e-65
Identity = 136/278 (48.92%), Postives = 180/278 (64.75%), Query Frame = 3
Query:    3 GPYLAIYLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQ-------------------------------------CKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGYTLVRNSTDLFRSFGF-DTQPVLIG-LIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAI 719
            G Y  IY W F  V+SLV++T+Y   IAPLF+KFTPLPEG+L+++IE +A S+ FPL K++VV+GS RSSHSNAY YGFFKNKRIVL+DTL+++                                     CKN EE++AV+ HELGHWKL HT+ + I  Q+ + L F  + ++    +LF +FGF D+QP LIG LIIFQ    P   ++SF L ++SR FEFQADAFAKKLG A  L + L+KL ++NL    +D  +S +HYSHPPL+ERL A+
Sbjct:  193 GDYFFIYAWLFTLVVSLVLVTIYADYIAPLFDKFTPLPEGKLKQEIEVMAKSIDFPLTKVYVVEGSKRSSHSNAYFYGFFKNKRIVLFDTLLEEYSVPNKDNQEESGMEARNEGEGDSEEVKAKVKNKKQGCKN-EEVLAVLGHELGHWKLGHTVKNIIISQMNSFLCFFLFAVLIGRRELFAAFGFYDSQPTLIGLLIIFQFIFSPYNEVLSFCLTVLSRRFEFQADAFAKKLGKAKDLYSALIKLNKDNLGFPVSDWLFSTWHYSHPPLLERLQAL 469          
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: STE24_YEAST (CAAX prenyl protease 1 OS=Saccharomyces cerevisiae GN=STE24 PE=1 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 4.310e-61
Identity = 118/250 (47.20%), Postives = 165/250 (66.00%), Query Frame = 3
Query:   21 YLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGF-FKNKRIVLYDTLIQQCKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGYTLVRNSTDLFRSFGFDTQ---------------PVLIGLIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAID 722
            Y+  F+FV+ ++ MT+ PV I P+FNKFTPL +GEL++ IE LA  + FPL K+FV+DGS RSSHSNAY  G  F +KRIVL+DTL+    + +EI AV+AHE+GHW+ NH +   I  Q+ T L F  +T +  +T  + +FGF  +               P++IG ++F   + P++  + F ++L+SR+ E+QADA+AKKLGY   L   L+ LQ +NLS MN DP YS+YHYSHP L ERL A+D
Sbjct:  197 YIMVFLFVVQILAMTIIPVFIMPMFNKFTPLEDGELKKSIESLADRVGFPLDKIFVIDGSKRSSHSNAYFTGLPFTSKRIVLFDTLVNS-NSTDEITAVLAHEIGHWQKNHIVNMVIFSQLHTFLIFSLFTSIYRNTSFYNTFGFFLEKSTGSFVDPVITKEFPIIIGFMLFNDLLTPLECAMQFVMSLISRTHEYQADAYAKKLGYKQNLCRALIDLQIKNLSTMNVDPLYSSYHYSHPTLAERLTALD 445          
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: STE24_SCHPO (Probable CAAX prenyl protease 1 OS=Schizosaccharomyces pombe GN=SPAC3H1.05 PE=2 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 7.120e-56
Identity = 108/241 (44.81%), Postives = 159/241 (65.98%), Query Frame = 3
Query:    3 GPYLAIYLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGYTLVRNSTDLFRSFGFDTQ-PVLIGLIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAID 722
            G    +Y W    V  L++ T+ P LI PLF KFTPL  G LR +IE+LA+S+ FPLKKL+V+D S RS+HSNA+ YG   NK IVL+DTL++      E++A++ HELGHW ++H + + I    ++L     +     +  L+ SF F T+ PV++GL++F   + P+  +++F  N VSR  E+QADAFAK+LGYA  L  GL+++ ++NLS +  D  Y++Y++SHP LV+RL AID
Sbjct:  227 GDNFIMYAWGAYIVFGLILQTIAPSLIMPLFYKFTPLENGSLRTQIEELAASINFPLKKLYVIDASRRSTHSNAFFYGLPWNKGIVLFDTLVKN-HTEPELIAILGHELGHWYMSHNLINTIIDYGMSLFHLFLFAAFIRNNSLYTSFNFITEKPVIVGLLLFSDALGPLSSILTFASNKVSRLCEYQADAFAKQLGYAKDLGDGLIRIHDDNLSPLEFDSLYTSYYHSHPILVDRLNAID 466          
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: FACE1_DICDI (CAAX prenyl protease 1 homolog OS=Dictyostelium discoideum GN=zmpste24 PE=3 SV=1)

HSP 1 Score: 202.986 bits (515), Expect = 1.390e-51
Identity = 110/245 (44.90%), Postives = 151/245 (61.63%), Query Frame = 3
Query:    3 GPYLAIYLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKK--LFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGYTLVRNSTDLFRSFGF--DTQPVLIGLIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVKLQEENLSAMNTDPWYSAYHYSHPPLVERLAAIDE 725
            GP L  Y W  +  ++L+ +T+YP  I PLFNKFTP+ +GEL E I  LA  + FP  K  +FVVD S R  H NAY YG F  KRIVLYDTL+ +  + EE++AV+ HE GH+K++HT+   + VQV  +     ++L+ N   L++ FGF      VL+GL +F     PI  + S  +N+ SR +EFQAD FA +LG+ ++    L KL  + L  +  DP YSAYH+SHP LVER   ID+
Sbjct:  176 GPQLWFYCWLLLVAITLLSITIYPTFIQPLFNKFTPV-DGELAESIFALAKRVGFPASKDTIFVVDNSKRDGHMNAYFYGLFGTKRIVLYDTLVNEL-DKEELLAVMGHEFGHYKMSHTLKQMLLVQVHLVTLLYAFSLLINDDQLYQQFGFVSSKDSVLVGLTLFMFLYSPIDRIFSLLINIFSRKYEFQADDFAVELGFLNSNH--LFKLHFKELGCLVYDPLYSAYHHSHPTLVERSNNIDK 416          
BLAST of DR406092 vs. ExPASy Swiss-Prot
Match: YHFN_BACSU (Uncharacterized metalloprotease yhfN OS=Bacillus subtilis GN=yhfN PE=3 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 5.893e-18
Identity = 71/240 (29.58%), Postives = 115/240 (47.92%), Query Frame = 3
Query:   18 IYLWAFMFVLSLVMMTLYPVLIAPLFNKFTPLPEGELREKIEKLASSLKFPLKKLFVVDGSTRSSHSNAYMYGFFKNKRIVLYDTLIQQCKNGEEIVAVIAHELGHWKLNHTMYSFIAVQVLTLLQFGGY----TLVRNSTDLFRS-FGFDTQPVLIGLIIFQHTVIPIQHLVSFGLNLVSRSFEFQADAFAKKLGYASALRAGLVK----LQEENLSAMNTDPWYSAYHYSHPPLVERL 710
            +Y W      SL +  + PV+I PL+N F PL   EL  KI +LA     P   ++ V+ S +++  NAY+ G   NKRIVL+DT + +  +  EI+ ++ HE+GH+ + H    +I +    L+   G+     L + +  L RS F  + +  L  L +       +   V+   N VSR  E +AD +  +L   +  R   VK    L    LS ++       +  SHP ++ER+
Sbjct:  177 LYAWLLTVPFSLFLFFIQPVIIDPLYNDFYPLKNKELESKILELADEANIPADHVYEVNMSEKTNALNAYVTGIGANKRIVLWDTTLNKL-DDSEILFIMGHEMGHYVMKHV---YIGLAGYLLVSLAGFYVIDKLYKRTVRLTRSMFHLEGRHDLAALPLLLLLFSVLSFAVTPFSNAVSRYQENKADQYGIEL---TENREAAVKTFQDLAVTGLSQVDPPVLVKIFRGSHPSIMERI 409          
The following BLAST results are available for this feature:
BLAST of DR406092 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 8
Match NameE-valueIdentityDescription
FACE1_ARATH2.243e-12691.39CAAX prenyl protease 1 homolog OS=Arabidopsis thal... [more]
FACE1_ORYSJ5.351e-12084.77CAAX prenyl protease 1 homolog OS=Oryza sativa sub... [more]
FACE1_HUMAN7.590e-6649.28CAAX prenyl protease 1 homolog OS=Homo sapiens GN=... [more]
FACE1_MOUSE1.295e-6548.92CAAX prenyl protease 1 homolog OS=Mus musculus GN=... [more]
STE24_YEAST4.310e-6147.20CAAX prenyl protease 1 OS=Saccharomyces cerevisiae... [more]
STE24_SCHPO7.120e-5644.81Probable CAAX prenyl protease 1 OS=Schizosaccharom... [more]
FACE1_DICDI1.390e-5144.90CAAX prenyl protease 1 homolog OS=Dictyostelium di... [more]
YHFN_BACSU5.893e-1829.58Uncharacterized metalloprotease yhfN OS=Bacillus s... [more]
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Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248L02 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248L02 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR406092 ID=DR406092; Name=DR406092; organism=Citrus sinensis; type=EST; length=735bp
GGGGTCCTTATCTGGCCATCTATCTTTGGGCATTTATGTTTGTTCTTTCT
CTTGTGATGATGACACTATACCCCGTTTTAATTGCCCCTCTGTTCAATAA
GTTTACTCCTCTTCCAGAAGGAGAGCTAAGGGAGAAAATTGAGAAACTTG
CTTCCTCCCTCAAGTTTCCTTTAAAGAAGTTGTTCGTAGTTGATGGATCC
ACAAGGTCAAGCCACAGCAATGCCTATATGTATGGATTCTTTAAGAATAA
GCGAATTGTCCTTTATGACACTTTGATACAACAGTGCAAAAATGGCGAGG
AAATTGTAGCTGTTATAGCTCATGAGCTTGGGCACTGGAAACTTAACCAT
ACAATGTACTCATTCATTGCTGTTCAGGTTCTTACACTCTTACAATTTGG
AGGATATACCCTTGTGAGGAACTCAACAGATCTCTTCCGAAGCTTTGGGT
TCGACACACAGCCAGTTCTCATTGGTCTAATCATCTTTCAGCATACTGTA
ATACCAATCCAACACTTAGTAAGCTTTGGTCTTAATCTTGTGAGCCGATC
TTTTGAATTTCAGGCTGATGCTTTTGCTAAGAAGCTTGGTTATGCCTCTG
CTCTTCGTGCTGGTCTTGTGAAACTACAGGAAGAAAATTTGTCGGCTATG
AATACGGATCCTTGGTACTCAGCTTATCACTATTCTCATCCACCACTTGT
CGAGCGACTGGCGGCAATTGATGAACCTGATAAGA
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