CN184530

Overview
NameCN184530
Unique NameCN184530
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length418
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:246..663. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK2_PEA (S-adenosylmethionine synthetase 2 OS=Pisum sativum GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 280.796 bits (717), Expect = 1.477e-75
Identity = 134/138 (97.10%), Postives = 136/138 (98.55%), Query Frame = 3 
Query:    3 TETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            TETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR+IGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPEDIGAGDQGHMFGYATDETPEFM
Sbjct:    3 TETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEDIGAGDQGHMFGYATDETPEFM 140
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK_BRARP (S-adenosylmethionine synthetase OS=Brassica rapa subsp. pekinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 277.33 bits (708), Expect = 1.633e-74
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE M
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK5_BRAJU (S-adenosylmethionine synthetase 5 OS=Brassica juncea GN=MSAMS5 PE=2 SV=1)

HSP 1 Score: 277.33 bits (708), Expect = 1.633e-74
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE M
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK3_BRAJU (S-adenosylmethionine synthetase 3 OS=Brassica juncea GN=MSAMS3 PE=2 SV=1)

HSP 1 Score: 277.33 bits (708), Expect = 1.633e-74
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE M
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK1_BRAJU (S-adenosylmethionine synthetase 1 OS=Brassica juncea GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 277.33 bits (708), Expect = 1.633e-74
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE M
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK2_ARATH (S-adenosylmethionine synthetase 2 OS=Arabidopsis thaliana GN=SAM2 PE=1 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 2.133e-74
Identity = 131/137 (95.62%), Postives = 134/137 (97.81%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA +DYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPE M
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK1_CATRO (S-adenosylmethionine synthetase 1 OS=Catharanthus roseus GN=SAMS1 PE=1 SV=1)

HSP 1 Score: 276.944 bits (707), Expect = 2.133e-74
Identity = 132/137 (96.35%), Postives = 135/137 (98.54%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDETPEFM
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPEFM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK_CARPA (S-adenosylmethionine synthetase OS=Carica papaya GN=SAMS PE=2 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 3.638e-74
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGFVSDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDETPEFM
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRSIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPEFM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK1_POPTR (S-adenosylmethionine synthetase 1 OS=Populus trichocarpa GN=METK1 PE=2 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 3.638e-74
Identity = 129/137 (94.16%), Postives = 135/137 (98.54%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTK K+DYEKIVRDTCR+IGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDETPE+M
Sbjct:    2 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKGKIDYEKIVRDTCRNIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPEYM 138
[Close]
BLAST of CN184530 vs. ExPASy Swiss-Prot
Match: METK4_HORVU (S-adenosylmethionine synthetase 4 OS=Hordeum vulgare GN=SAM4 PE=2 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 6.206e-74
Identity = 130/137 (94.89%), Postives = 134/137 (97.81%), Query Frame = 3 
Query:    6 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPEFM 416
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL +DP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGFVS+DVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDETPEFM
Sbjct:    5 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPEFM 141
The following BLAST results are available for this feature:
BLAST of CN184530 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK2_PEA1.477e-7597.10S-adenosylmethionine synthetase 2 OS=Pisum sativum... [more]
METK_BRARP1.633e-7496.35S-adenosylmethionine synthetase OS=Brassica rapa s... [more]
METK5_BRAJU1.633e-7496.35S-adenosylmethionine synthetase 5 OS=Brassica junc... [more]
METK3_BRAJU1.633e-7496.35S-adenosylmethionine synthetase 3 OS=Brassica junc... [more]
METK1_BRAJU1.633e-7496.35S-adenosylmethionine synthetase 1 OS=Brassica junc... [more]
METK2_ARATH2.133e-7495.62S-adenosylmethionine synthetase 2 OS=Arabidopsis t... [more]
METK1_CATRO2.133e-7496.35S-adenosylmethionine synthetase 1 OS=Catharanthus ... [more]
METK_CARPA3.638e-7496.35S-adenosylmethionine synthetase OS=Carica papaya G... [more]
METK1_POPTR3.638e-7494.16S-adenosylmethionine synthetase 1 OS=Populus trich... [more]
METK4_HORVU6.206e-7494.89S-adenosylmethionine synthetase 4 OS=Hordeum vulga... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0005K14_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0005K14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184530 ID=CN184530; Name=CN184530; organism=Citrus sinensis; type=EST; length=418bp
AGACGGAGACCTTTCTATTCACCTCTGAATCTGTGAACGAGGGTCACCCC
GACAAGTTGTGCGACCAGATCTCTGATGCGGTGCTTGATGCCTGCCTTGA
GCAGGATCCTGAGAGCAAGGTTGCCTGTGAGACATGCACCAAGACCAACA
TGGTCATGGTCTTTGGAGAGATTACCACCAAGGCCAAGGTAGACTATGAG
AAGATTGTTCGCGACACTTGCCGTTCCATTGGATTTGTTTCTGATGATGT
GGGTCTTGATGCCGATCACTGCAAAGTCCTGGTCAATATTGAGCAACAAA
GCCCTGATATTGCCCAGGGTGTTCATGGCCACTTTACAAAGCGCCCTGAG
GACATTGGTGCTGGTGACCAGGGCCATATGTTTGGTTATGCTACTGATGA
GACCCCTGAATTTATGCC
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