EY679003

Overview
NameEY679003
Unique NameEY679003
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SPIOL (Formate--tetrahydrofolate ligase OS=Spinacia oleracea PE=1 SV=3)

HSP 1 Score: 180.644 bits (457), Expect = 3.522e-55
Identity = 86/102 (84.31%), Postives = 98/102 (96.08%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GGKGAVDLGIAVQ+ACENVTQPL+FLYPLD+SIKEKI+ IA+SYGA+GVEYSE+AEK+IEMY+ QGFS LPICMA TQYSFSH+AAEKGAP+GFILPIRDV+
Sbjct:  493 GGKGAVDLGIAVQKACENVTQPLRFLYPLDISIKEKIEAIAKSYGAAGVEYSEQAEKKIEMYSKQGFSNLPICMAKTQYSFSHNAAEKGAPSGFILPIRDVR 594

HSP 2 Score: 56.6102 bits (135), Expect = 3.522e-55
Identity = 25/27 (92.59%), Postives = 25/27 (92.59%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            R SIGAGFIYPLVGTMSTMPGLP RPC
Sbjct:  594 RGSIGAGFIYPLVGTMSTMPGLPTRPC 620
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_ARATH (Formate--tetrahydrofolate ligase OS=Arabidopsis thaliana GN=THFS PE=2 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 4.163e-53
Identity = 79/101 (78.22%), Postives = 96/101 (95.05%), Query Frame = 2 
Query:   38 GKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GKGAVDLGIAV++AC+N+TQPL+FLYPLD+ IK+KI+ IA+SYGASGVEYS++AEKQIEMYT QGFS LPICM+ TQYSFSHDA++KGAP+GF+LPIRDV+
Sbjct:  491 GKGAVDLGIAVEKACQNITQPLRFLYPLDIGIKDKIEAIAKSYGASGVEYSDQAEKQIEMYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVR 591

HSP 2 Score: 56.6102 bits (135), Expect = 4.163e-53
Identity = 25/27 (92.59%), Postives = 25/27 (92.59%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            R SIGAGFIYPLVGTMSTMPGLP RPC
Sbjct:  591 RGSIGAGFIYPLVGTMSTMPGLPTRPC 617
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TM_BOVIN (Monofunctional C1-tetrahydrofolate synthase, mitochondrial OS=Bos taurus GN=MTHFD1L PE=2 SV=2)

HSP 1 Score: 101.679 bits (252), Expect = 4.486e-32
Identity = 55/105 (52.38%), Postives = 71/105 (67.62%), Query Frame = 2 
Query:   32 MGGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            +GGKG+VDL  AV+ A    ++  +FLY + + I EKI TIA+S YGA  +E S EA+ +I+ YT QGF  LPICMA T  S SH   +KG P GFILPI DV++
Sbjct:  830 IGGKGSVDLAWAVREAASKESR-FQFLYDVQLPIVEKIRTIAQSVYGAKDIELSPEAQSKIDRYTEQGFGNLPICMAKTHLSLSHQPDKKGVPKGFILPISDVRA 933

HSP 2 Score: 58.151 bits (139), Expect = 4.486e-32
Identity = 26/27 (96.30%), Postives = 26/27 (96.30%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            RASIGAGFIYPLVGTMSTMPGLP RPC
Sbjct:  932 RASIGAGFIYPLVGTMSTMPGLPTRPC 958
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TC_MOUSE (C-1-tetrahydrofolate synthase, cytoplasmic OS=Mus musculus GN=Mthfd1 PE=1 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 3.711e-31
Identity = 53/104 (50.96%), Postives = 70/104 (67.31%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GG+GA+ L  AVQRA +      + LY L +SI++KI  IA R YGA  +E   EA+ + E+YT QGF  LPICMA T  S SH+  +KG PTGF+LPIRD+++
Sbjct:  791 GGQGALALAQAVQRASQ-APSSFQLLYDLKLSIEDKIRIIAQRIYGADDIELLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRA 893

HSP 2 Score: 56.9954 bits (136), Expect = 3.711e-31
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            RAS+GAGF+YPLVGTMSTMPGLP RPC
Sbjct:  892 RASVGAGFLYPLVGTMSTMPGLPTRPC 918
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TC_RAT (C-1-tetrahydrofolate synthase, cytoplasmic OS=Rattus norvegicus GN=Mthfd1 PE=1 SV=3)

HSP 1 Score: 98.5969 bits (244), Expect = 8.193e-31
Identity = 51/104 (49.04%), Postives = 70/104 (67.31%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GG+GA+ L  AVQRA +      + LY L +S+++KI  IA+  YGA  +E   EA+ + E+YT QGF  LPICMA T  S SH+  +KG PTGF+LPIRD+++
Sbjct:  791 GGQGALALAQAVQRASQ-APSSFQLLYDLKLSVEDKIRIIAQKIYGADDIELLPEAQNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRA 893

HSP 2 Score: 56.9954 bits (136), Expect = 8.193e-31
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            RAS+GAGF+YPLVGTMSTMPGLP RPC
Sbjct:  892 RASVGAGFLYPLVGTMSTMPGLPTRPC 918
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TC_HUMAN (C-1-tetrahydrofolate synthase, cytoplasmic OS=Homo sapiens GN=MTHFD1 PE=1 SV=3)

HSP 1 Score: 98.5969 bits (244), Expect = 8.193e-31
Identity = 52/104 (50.00%), Postives = 69/104 (66.35%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKGA+ L  AVQRA +      + LY L + +++KI  IA+  YGA  +E   EA+ + E+YT QGF  LPICMA T  S SH+  +KG PTGFILPIRD+++
Sbjct:  791 GGKGALALAQAVQRAAQ-APSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRA 893

HSP 2 Score: 56.9954 bits (136), Expect = 8.193e-31
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            RAS+GAGF+YPLVGTMSTMPGLP RPC
Sbjct:  892 RASVGAGFLYPLVGTMSTMPGLPTRPC 918
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TM_HUMAN (Monofunctional C1-tetrahydrofolate synthase, mitochondrial OS=Homo sapiens GN=MTHFD1L PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.066e-30
Identity = 53/105 (50.48%), Postives = 70/105 (66.67%), Query Frame = 2 
Query:   32 MGGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            +GGKG+VDL  AV+ A    ++  +FLY + V I +KI TIA++ YGA  +E S EA+ +I+ YT QGF  LPICMA T  S SH   +KG P  FILPI DV++
Sbjct:  833 VGGKGSVDLARAVREAASKRSR-FQFLYDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQGFGNLPICMAKTHLSLSHQPDKKGVPRDFILPISDVRA 936

HSP 2 Score: 58.151 bits (139), Expect = 1.066e-30
Identity = 26/27 (96.30%), Postives = 26/27 (96.30%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            RASIGAGFIYPLVGTMSTMPGLP RPC
Sbjct:  935 RASIGAGFIYPLVGTMSTMPGLPTRPC 961
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TC_PONAB (C-1-tetrahydrofolate synthase, cytoplasmic OS=Pongo abelii GN=MTHFD1 PE=2 SV=3)

HSP 1 Score: 96.2857 bits (238), Expect = 3.993e-30
Identity = 50/104 (48.08%), Postives = 68/104 (65.38%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GG GA+ L  AVQRA +      + LY L + +++KI  IA+  YGA  +E   EA+ + E+YT QGF  LP+CMA T  S SH+  +KG PTGFILPIRD+++
Sbjct:  791 GGNGALALAQAVQRAAQ-APSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPVCMAKTHLSLSHNPEQKGVPTGFILPIRDIRA 893

HSP 2 Score: 56.9954 bits (136), Expect = 3.993e-30
Identity = 24/27 (88.89%), Postives = 26/27 (96.30%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            RAS+GAGF+YPLVGTMSTMPGLP RPC
Sbjct:  892 RASVGAGFLYPLVGTMSTMPGLPTRPC 918
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TM_MOUSE (Monofunctional C1-tetrahydrofolate synthase, mitochondrial OS=Mus musculus GN=Mthfd1l PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 5.195e-30
Identity = 52/104 (50.00%), Postives = 67/104 (64.42%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKG+VDL  AV+ A  N     +FLY + + I EKI  IA++ YGA  +E S EA+ +I+ YT QGF  LPICMA T  S SH+  +KG P  F LPI DV++
Sbjct:  833 GGKGSVDLARAVREAA-NKRSRFQFLYDVQLPIVEKIRVIAQTVYGAKDIELSPEAQSKIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFTLPISDVRA 935

HSP 2 Score: 58.151 bits (139), Expect = 5.195e-30
Identity = 26/27 (96.30%), Postives = 26/27 (96.30%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPC 419
            RASIGAGFIYPLVGTMSTMPGLP RPC
Sbjct:  934 RASIGAGFIYPLVGTMSTMPGLPTRPC 960
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_ROSS1 (Formate--tetrahydrofolate ligase OS=Roseiflexus sp. (strain RS-1) GN=fhs PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.736e-27
Identity = 54/105 (51.43%), Postives = 73/105 (69.52%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKF--LYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G  DL  AV  ACE    P KF  LYPLD+S+++KI+T+A + YGA+ VEY+ EA +Q+  +  QG+  LPICMA TQ S SHD   +GAP+G++ PIR+V+
Sbjct:  421 GGAGGYDLAQAVMAACE---MPGKFTPLYPLDMSLRDKIETLATKVYGAARVEYTPEASRQLTRFEQQGYGALPICMAKTQLSISHDPKLRGAPSGYVFPIREVR 522

HSP 2 Score: 40.817 bits (94), Expect = 1.736e-27
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            R + GAGFIYPL G + TMPGLP  P   R
Sbjct:  522 RLAAGAGFIYPLAGDIRTMPGLPAHPAAER 551
The following BLAST results are available for this feature:
BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
FTHS_SPIOL3.522e-5584.31Formate--tetrahydrofolate ligase OS=Spinacia olera... [more]
FTHS_ARATH4.163e-5378.22Formate--tetrahydrofolate ligase OS=Arabidopsis th... [more]
C1TM_BOVIN4.486e-3252.38Monofunctional C1-tetrahydrofolate synthase, mitoc... [more]
C1TC_MOUSE3.711e-3150.96C-1-tetrahydrofolate synthase, cytoplasmic OS=Mus ... [more]
C1TC_RAT8.193e-3149.04C-1-tetrahydrofolate synthase, cytoplasmic OS=Ratt... [more]
C1TC_HUMAN8.193e-3150.00C-1-tetrahydrofolate synthase, cytoplasmic OS=Homo... [more]
C1TM_HUMAN1.066e-3050.48Monofunctional C1-tetrahydrofolate synthase, mitoc... [more]
C1TC_PONAB3.993e-3048.08C-1-tetrahydrofolate synthase, cytoplasmic OS=Pong... [more]
C1TM_MOUSE5.195e-3050.00Monofunctional C1-tetrahydrofolate synthase, mitoc... [more]
FTHS_ROSS11.736e-2751.43Formate--tetrahydrofolate ligase OS=Roseiflexus sp... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA
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