DR908624

Overview
NameDR908624
Unique NameDR908624
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length605
Libraries
Library NameType
Citrus sinensis phloemcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 2.388e-66
Identity = 117/183 (63.93%), Postives = 145/183 (79.23%), Query Frame = 3
Query:    3 YGDLIWDLLVSGLLPEKVCQQIGLCAFNGAEYVSTGIKTVVEKENVSAGDS---AVCSACEMTVVWVQNQLKQKQTKEKVLSYINELCDSLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 542
            YG  I DLL+S   P+K+C QI LC F+G   VS GI++VV++    + DS    +CS CEMTVVW+QNQL+Q QTKE++++YINELCD +P+PMG+S +DCG++ +MP VSFTIG KIF+L+PE+YILK GEG    CISGF AFD+PPPRGPLWILGDVFMG YHTVFD GKLR+G AEAA
Sbjct:  331 YGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIESVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDVFMGRYHTVFDFGKLRVGSAEAA 513          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 239.965 bits (611), Expect = 7.191e-63
Identity = 113/186 (60.75%), Postives = 141/186 (75.81%), Query Frame = 3
Query:    3 YGDLIWDLLVSGLLPEKVCQQIGLCAFNGAEYVSTGIKTVVEKENVSAGDS------AVCSACEMTVVWVQNQLKQKQTKEKVLSYINELCDSLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 542
            YG  I DLL++   P K+C Q+GLC F+G   VS GIK+VV+ E   AG+S       +C+ACEM VVW+QNQL Q +T++ +L+YIN+LCD LP+PMGES +DCG + +MP +SFTIG K F L PE+YILK GEG A  CISGF A D+PPPRGPLWILGDVFMG YHTVFD GK+R+GFA++A
Sbjct:  327 YGQQILDLLLAETQPSKICSQVGLCTFDGKHGVSAGIKSVVDDE---AGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGAYHTVFDYGKMRVGFAKSA 509          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 1.227e-62
Identity = 111/183 (60.66%), Postives = 139/183 (75.96%), Query Frame = 3
Query:    3 YGDLIWDLLVSGLLPEKVCQQIGLCAFNGAEYVSTGIKTVVEKENVSAGD---SAVCSACEMTVVWVQNQLKQKQTKEKVLSYINELCDSLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 542
            YG  I DLL++   P+K+C Q+GLC F+G   VS GI++VV+ E V +       +CSACEM VVW+QNQL Q +T++ +L Y+N+LC+ LP+PMGES +DCG + +MP++ FTIG K F L PE+YILK GEG A  CISGF A D+PPPRGPLWILGDVFMG YHTVFD GKLRIGFA+AA
Sbjct:  326 YGQQILDLLLAETQPKKICSQVGLCTFDGTRGVSAGIRSVVDDEPVKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPSPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 238.039 bits (606), Expect = 2.733e-62
Identity = 109/180 (60.56%), Postives = 143/180 (79.44%), Query Frame = 3
Query:    3 YGDLIWDLLVSGLLPEKVCQQIGLCAFNGAEYVSTGIKTVVEKENVSAGDSAVCSACEMTVVWVQNQLKQKQTKEKVLSYINELCDSLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 542
            YG++I +LL++   P+KVC Q+GLC F+G   VS GI++VV+KEN+  G  A+CS CEM VVW++NQL++ +TKE +L+Y N+LC+ LP+P GES + C +I  MPN++FTI +K F L+PEQYI+K  +G   VCISGFMAFD+PPPRGPLWILGDVFMG YHTVFD GK RIGFA++A
Sbjct:  319 YGEMILNLLIAQTDPQKVCSQVGLCMFDGKRSVSNGIESVVDKENL--GSDAMCSVCEMAVVWIENQLRENKTKELILNYANQLCERLPSPNGESTVSCHQISKMPNLAFTIANKTFILTPEQYIVKLEQGGQTVCISGFMAFDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 217.624 bits (553), Expect = 3.822e-56
Identity = 99/181 (54.70%), Postives = 135/181 (74.59%), Query Frame = 3
Query:    3 YGDLIWDLLVSGLLPEKVCQQIGLCAFNGAEYVSTGIKTVVEK-ENVSAGDSAVCSACEMTVVWVQNQLKQKQTKEKVLSYINELCDSLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 542
            YG  + ++L+S   PEK+C Q+ LC+F+G+   S  I++VV+K +  S+G    C  C   VVW+QNQ++Q +T+E +++Y+++LC+ LP+PMGES +DC  + +MPN++FT+G K FNLSPEQY+LK GEG    CISGF A D+ PP GPLWILGDVFMG YHTVFD G LR+GFAEAA
Sbjct:  293 YGKSMIEMLLSEEQPEKICSQMKLCSFDGSHDTSMIIESVVDKSKGKSSGLPMRCVPCARWVVWMQNQIRQNETEENIINYVDKLCERLPSPMGESAVDCSSLSSMPNIAFTVGGKTFNLSPEQYVLKVGEGATAQCISGFTAMDVAPPHGPLWILGDVFMGQYHTVFDYGNLRVGFAEAA 473          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: CATD_CLUHA (Cathepsin D OS=Clupea harengus GN=ctsd PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 4.557e-25
Identity = 47/93 (50.54%), Postives = 69/93 (74.19%), Query Frame = 3
Query:  261 SLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEA 539
            ++P   GE +IDC ++PT+P +SF +G K ++L+ EQY+LK  +G   +C+SG M  ++PPP GPLWILGDVF+G Y+TVFD    R+GFA++
Sbjct:  303 AIPLIQGEYMIDCKKVPTLPTISFNVGGKTYSLTGEQYVLKESQGGKTICLSGLMGLEIPPPAGPLWILGDVFIGQYYTVFDRESNRVGFAKS 395          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: ASPP_AEDAE (Lysosomal aspartic protease OS=Aedes aegypti GN=AAEL006169 PE=1 SV=2)

HSP 1 Score: 110.538 bits (275), Expect = 6.580e-24
Identity = 56/137 (40.88%), Postives = 79/137 (57.66%), Query Frame = 3
Query:  132 ENVSAGDSAVCS-ACEMTVVWVQNQLKQKQTKEKVLSYINELCDSLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEA 539
            ++V  GD+  C+  CE  +      L      E  ++ IN+     P   GE ++DC  IP +P +SF +G K F+L    Y+L+  +    +C+SGFM  D+PPP GPLWILGDVF+G Y+T FD G  R+GFA A
Sbjct:  253 DSVKVGDTEFCNNGCE-AIADTGTSLIAGPVSE--VTAINKAIGGTPIMNGEYMVDCSLIPKLPKISFVLGGKSFDLEGADYVLRVAQMGKTICLSGFMGIDIPPPNGPLWILGDVFIGKYYTEFDMGNDRVGFATA 386          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: CATD_CHIHA (Cathepsin D OS=Chionodraco hamatus GN=ctsd PE=1 SV=2)

HSP 1 Score: 110.153 bits (274), Expect = 8.593e-24
Identity = 55/141 (39.01%), Postives = 87/141 (61.70%), Query Frame = 3
Query:  123 VEKENVSAGDS-AVCSA-CEMTVVWVQNQLKQKQTKEKVLSYINELCDSLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEA 539
            +  ++++ GD  ++C+  CE  V    + +     + K L    +   + P   GE +++C  +P++P +SFT+G +++ L+ EQYILK  +    +C+SGFM  D+P P GPLWILGDVFMG Y+TVFD    R+GFA+A
Sbjct:  258 IRVDSMAVGDQLSLCTGGCEAIVDSGTSLITGPSVEVKALQ---KAIGAFPLIQGEYMVNCDTVPSLPVISFTVGGQVYTLTGEQYILKVTQAGKTMCLSGFMGLDIPAPAGPLWILGDVFMGQYYTVFDRDANRVGFAKA 395          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 3.266e-23
Identity = 48/94 (51.06%), Postives = 65/94 (69.15%), Query Frame = 3
Query:  261 SLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 542
            ++P   GE +I C ++ T+P ++  +G K + LSPE Y LK  +    +C+SGFM  D+PPP GPLWILGDVF+G Y+TVFD    R+GFAEAA
Sbjct:  317 AVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAA 410          
BLAST of DR908624 vs. ExPASy Swiss-Prot
Match: CATD_PIG (Cathepsin D OS=Sus scrofa GN=CTSD PE=1 SV=2)

HSP 1 Score: 107.842 bits (268), Expect = 4.265e-23
Identity = 48/94 (51.06%), Postives = 66/94 (70.21%), Query Frame = 3
Query:  261 SLPNPMGESIIDCGRIPTMPNVSFTIGDKIFNLSPEQYILKTGEGIAEVCISGFMAFDLPPPRGPLWILGDVFMGVYHTVFDSGKLRIGFAEAA 542
            ++P   GE +I C ++P++P+V+ T+G K + LS E Y LK  +    +C+SGFM  D+PPP GPLWILGDVF+G Y+TVFD    R+G AEAA
Sbjct:  252 AVPLIQGEYMIPCEKVPSLPDVTVTLGGKKYKLSSENYTLKVSQAGQTICLSGFMGMDIPPPGGPLWILGDVFIGRYYTVFDRDLNRVGLAEAA 345          
The following BLAST results are available for this feature:
BLAST of DR908624 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 65
Match NameE-valueIdentityDescription
ASPR_CUCPE2.388e-6663.93Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1[more]
ASPR1_ORYSJ7.191e-6360.75Aspartic proteinase oryzasin-1 OS=Oryza sativa sub... [more]
ASPR_HORVU1.227e-6260.66Phytepsin OS=Hordeum vulgare PE=1 SV=1[more]
ASPRX_ORYSJ2.733e-6260.56Aspartic proteinase OS=Oryza sativa subsp. japonic... [more]
CYPR1_CYNCA3.822e-5654.70Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO... [more]
CATD_CLUHA4.557e-2550.54Cathepsin D OS=Clupea harengus GN=ctsd PE=1 SV=1[more]
ASPP_AEDAE6.580e-2440.88Lysosomal aspartic protease OS=Aedes aegypti GN=AA... [more]
CATD_CHIHA8.593e-2439.01Cathepsin D OS=Chionodraco hamatus GN=ctsd PE=1 SV... [more]
CATD_HUMAN3.266e-2351.06Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1[more]
CATD_PIG4.265e-2351.06Cathepsin D OS=Sus scrofa GN=CTSD PE=1 SV=2[more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_16752 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-44_E08 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR908624 ID=DR908624; Name=DR908624; organism=Citrus sinensis; type=EST; length=605bp
AGTATGGGGATTTAATATGGGATCTCTTAGTATCAGGGCTACTACCTGAG
AAAGTATGTCAGCAGATTGGTTTATGTGCTTTCAATGGCGCTGAATATGT
GAGTACTGGGATCAAGACCGTGGTTGAGAAGGAAAACGTATCAGCCGGGG
ATAGTGCTGTGTGCAGTGCTTGTGAAATGACCGTGGTTTGGGTCCAGAAT
CAGCTAAAACAAAAGCAAACAAAGGAGAAAGTACTCAGTTATATAAATGA
GCTCTGCGACAGCCTGCCAAATCCGATGGGAGAATCAATTATTGACTGTG
GTCGCATTCCAACCATGCCAAATGTTTCATTCACCATCGGAGACAAAATT
TTCAACCTCTCTCCAGAGCAGTATATTCTGAAAACCGGAGAAGGGATTGC
AGAAGTCTGCATCAGTGGCTTCATGGCTTTCGACCTGCCTCCTCCAAGGG
GTCCTCTGTGGATTCTGGGAGATGTATTCATGGGGGTTTATCACACTGTT
TTCGACTCTGGTAAGCTCCGGATCGGCTTTGCTGAGGCTGCGTAATGATC
TACTTACTGTCAGTTTTTATGTAGTAGCAGCGATCATCACCTGCTCATAT
GAGGA
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