Prolonged somatic transposition in citrus: The autonomous Ac transposable element remains active in the citrus genome for several years

Publication Overview
TitleProlonged somatic transposition in citrus: The autonomous Ac transposable element remains active in the citrus genome for several years
AuthorsTrainin T, Lipsky A, Levy A, Holland D
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume130
Issue1
Year2005
Page(s)95-101
CitationTrainin T, Lipsky A, Levy A, Holland D. Prolonged somatic transposition in citrus: The autonomous Ac transposable element remains active in the citrus genome for several years. Journal of the American Society for Horticultural Science. 2005; 130(1):95-101.

Abstract

The maize transposable element Activator (Ac) has been shown to be active in a number of dicots, including arabidopsis Arabidopsis thaliana (L.) Heynh., tobacco (Nicotiana tabacum L.), tomato (Lycopersicon esculentum Mill.), potato (Solanum tuberosum L.), and aspen (Populus tremuloides Michx.). However, no information is available on somatic transposition in any plant during several years of growth and development. It is not known how transposition affects genetic variability among vegetative parts that have developed during a long period of growth. In order to explore the possibility of using somatic Ac transposition for gene tagging and mutagenesis in fruit trees, a derivative of the maize Ac transposable element was introduced into 'Duncan' grapefruit (Citrus paradisi Macf.) by Agrobacterium tumefaciens (Smith & Towns.) Conn.-mediated stable transformation. Genetically identical 4-year-old sibling trees were established by grafting one of the transformants on Troyer citrange Citrus sinensis (L.) Osbec. x Poncirus trifoliate (L.) Ras. rootstocks. We demonstrated that the Ac element was active upon transformation in citrus (Citrus L.) trees and that transposition can create genetic variability among tree siblings and among leaves collected from different parts of the same tree. Ac was still active among propagated plants 4 years after transformation, clearly indicating that it is capable of maintaining itself in citrus trees for a long period of time. The observation of different integration patterns in different parts of the same tree and within tree siblings originating from the same transformant suggests that an Ac-based mutagenesis system could be very useful in creating somatic mutations in citrus trees.
Features
This publication contains information about 12 features:
Feature NameUniquenameType
35S forward35S forwardgenetic_marker
Ac1Ac1genetic_marker
Ac1'Ac1'genetic_marker
Ac138Ac138genetic_marker
Ac2Ac2genetic_marker
BamHI adapterBamHI adaptergenetic_marker
MseI adapterMseI adaptergenetic_marker
MseI primerMseI primergenetic_marker
TAc 126TAc 126genetic_marker
TAc 44TAc 44genetic_marker
Tdsf1Tdsf1genetic_marker
uidA forwarduidA forwardgenetic_marker
Stocks
This publication contains information about 1 stocks:
Stock NameUniquenameType
DuncanDuncancultivar
Properties
Additional details for this publication include:
Property NameValue
Publication Date2005
Published Location|||
Language Abbreng
Publication TypeJournal Article
KeywordsCitrus paradisi, grapefruits, fruit crops, transposons, transposition (genetics), genetic variation, plant growth, plant development, insertional mutagenesis
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3689269