CX300464

Overview
NameCX300464
Unique NameCX300464
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length583
Libraries
Library NameType
SaltLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFRH_SOLTU (Isoflavone reductase homolog OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 4.005e-76
Identity = 141/194 (72.68%), Postives = 166/194 (85.57%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQEA 582
            D+ +HESLVKAIKQVDVVISTVGH L+ADQVK+IAAIKEAGNVKRFFPSEFG D DR+H AVEPAK+ ++ KA+IRR VEAEGIP+TYVA++   G+ LPNL+QP A  PP DKVVILG GN KAV+NKE+D+ T+TI AVDDP+TLNK LYI+PP NI + N+LVS+WE+K GK LER YV EEQ+LKNIQEA
Sbjct:   63 DLYDHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRVH-AVEPAKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLAQPGAAGPPNDKVVILGHGNTKAVFNKEEDIGTYTINAVDDPKTLNKILYIKPPHNIITLNELVSLWEKKTGKNLERLYVPEEQVLKNIQEA 255          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFRH_TOBAC (Isoflavone reductase homolog A622 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 4.438e-67
Identity = 127/193 (65.80%), Postives = 151/193 (78.24%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQE 579
            D+ N ESL+KAIKQVDVVISTVG     DQV II AIKEAGN+KRF PSEFG D D    A+EPA S + +K +IRR +EAEGIPYTYV        FLPNL Q EA  PPRDKVVI GDGNPKA+Y KE+D+AT+TI+AVDDPRTLNK L+++PP NI SFN++VS+WE KIGKTLE+ Y+SEE +L+ +QE
Sbjct:   65 DISNQESLLKAIKQVDVVISTVGGQQFTDQVNIIKAIKEAGNIKRFLPSEFGFDVDHAR-AIEPAASLFALKVRIRRMIEAEGIPYTYVICNWFADFFLPNLGQLEAKTPPRDKVVIFGDGNPKAIYVKEEDIATYTIEAVDDPRTLNKTLHMRPPANILSFNEIVSLWEDKIGKTLEKLYLSEEDILQIVQE 256          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFRH_ARATH (Isoflavone reductase homolog P3 OS=Arabidopsis thaliana GN=At1g75280 PE=1 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 9.887e-67
Identity = 130/196 (66.33%), Postives = 156/196 (79.59%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQ--PEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQEA 582
            D+ +HESLVKAIKQVDVVISTVG   I DQ KII+AIKEAGNVKRF PSEFG+D DR   AVEPAKS +  K +IRR +EAEGIPYTY  +    G++LP L Q  P  T+PPRDKV ILGDGN KAV NKE+D+A +TIKAVDDPRTLNK LYI+P  N  S N++V++WE+KIGK+LE+ ++ EEQLLK+IQE+
Sbjct:   64 DLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRT-SAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTHLPEEQLLKSIQES 258          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFR_MEDSA (Isoflavone reductase OS=Medicago sativa GN=IFR PE=1 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 1.578e-64
Identity = 120/194 (61.86%), Postives = 152/194 (78.35%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQEA 582
            D+ +HE+LVKAIKQVD+VI   G  LI DQVKII AIKEAGNVK+FFPSEFG+D DR H AVEP +  ++ KA IRR +EAEG+PYTY+  +   G+FL NL+Q + T PPRDKVVILGDGN K  Y  E DV TFTI+A +DP TLNK ++I+ P N  + N+++++WE+KIGKTLE+ YVSEEQ+LK+IQE+
Sbjct:   74 DINDHETLVKAIKQVDIVICAAGRLLIEDQVKIIKAIKEAGNVKKFFPSEFGLDVDR-HEAVEPVRQVFEEKASIRRVIEAEGVPYTYLCCHAFTGYFLRNLAQLDTTDPPRDKVVILGDGNVKGAYVTEADVGTFTIRAANDPNTLNKAVHIRLPENYLTQNEVIALWEKKIGKTLEKTYVSEEQVLKDIQES 266          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFR_CICAR (Isoflavone reductase OS=Cicer arietinum GN=IFR PE=1 SV=1)

HSP 1 Score: 244.973 bits (624), Expect = 2.062e-64
Identity = 121/191 (63.35%), Postives = 151/191 (79.06%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNI 573
            D+ +HE+LVKAIKQVD VI T G  LI DQVKII AIKEAGNVKRFFPSEFG+D DR H AV+P +  +D KA IRR VEAEG+PYTY+  +   G+FL NL+Q +AT PPRDKV+ILGDGN K  Y  E DV T+TI+A +DPRTLNK ++I+ P N  + N++VS+WE+KIGKTLE+ Y+SEE++LK+I
Sbjct:   74 DMNDHEALVKAIKQVDTVICTFGRLLILDQVKIIKAIKEAGNVKRFFPSEFGLDVDR-HDAVDPVRPVFDEKASIRRVVEAEGVPYTYLCCHAFTGYFLRNLAQFDATEPPRDKVIILGDGNVKGAYVTEADVGTYTIRAANDPRTLNKAVHIRLPHNYLTSNEVVSLWEKKIGKTLEKSYISEEKVLKDI 263          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFR_PEA (Isoflavone reductase OS=Pisum sativum GN=IFR PE=2 SV=1)

HSP 1 Score: 240.736 bits (613), Expect = 3.888e-63
Identity = 117/192 (60.94%), Postives = 149/192 (77.60%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQ 576
            D+ +HE+LV AIKQVD VI   G  LI DQVK+I AIKEAGNVKRFFPSEFG+D DR H AVEP +  ++ KA IRR VE+EG+PYTY+  +   G+FL NL+Q +AT PPRDKVVILGDGN +  Y  E DV T+TI+A +DP TLNK ++I+ P N  + N+++++WE+KIGKTLE+ YVSEEQ+LK+IQ
Sbjct:   74 DINDHETLVNAIKQVDTVICAAGRLLIEDQVKVIKAIKEAGNVKRFFPSEFGLDVDR-HDAVEPVRQVFEEKASIRRVVESEGVPYTYLCCHAFTGYFLRNLAQIDATDPPRDKVVILGDGNVRGAYVTEADVGTYTIRAANDPNTLNKAVHIRLPNNYLTANEVIALWEKKIGKTLEKTYVSEEQVLKDIQ 264          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFRH_MAIZE (Isoflavone reductase homolog IRL OS=Zea mays GN=IRL PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 6.012e-56
Identity = 112/194 (57.73%), Postives = 136/194 (70.10%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQEA 582
            D+ +  SLV A+K  DVVIS +G   IADQ +++ AIKEAGNVKRFFPSEFG+D DR  G VEPAKS    K  IRRA EA GIPYTY  +    G  LP + Q  A  PP DK V+LGDG+ KAV+ +E D+AT+T+ A DDPR  NK LYI+PP N  S N+L+S+WE+K GKT  REYV EE +LK IQE+
Sbjct:   64 DLYDQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRT-GIVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQVLAPGPPADKAVVLGDGDTKAVFVEEGDIATYTVLAADDPRAENKVLYIKPPANTLSHNELLSLWEKKTGKTFRREYVPEEAVLKQIQES 256          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IFRH_LUPAL (Isoflavone reductase homolog OS=Lupinus albus PE=2 SV=1)

HSP 1 Score: 196.823 bits (499), Expect = 6.438e-50
Identity = 96/195 (49.23%), Postives = 135/195 (69.23%), Query Frame = 1
Query:   10 NHESLVKAIKQVDVVISTVG------HTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQ 576
            +H+SLV A+K VDVVI T+       H L+  Q+K++ AIK+AGN+KRF PSEFG+D   +  A+EP + T+D K  +R+A+E   IP+TY+++    G+F  NLSQ +   PPRDKV++ GDGN K VY  EDDVAT+TIK +DDPRTLNK +Y++PP NI +  +L+  WE  IGK LE+  +SE+  L  ++
Sbjct:   65 DHKSLVDAVKLVDVVICTMSGVHFRSHNLLT-QLKLVEAIKDAGNIKRFLPSEFGMDPALMGHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLLYGDGNVKPVYMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTHKELIEKWEELIGKQLEKNSISEKDFLSTLK 258          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: IGS1_PETHY (Isoeugenol synthase 1 OS=Petunia hybrida GN=IGS1 PE=1 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 8.428e-42
Identity = 89/189 (47.09%), Postives = 126/189 (66.67%), Query Frame = 1
Query:    1 DVLNHESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLK 567
            ++  H+ LV   K+VD+VIST+      +Q+K+I AIKEAGN+KRF PSEFG + DR+  A+   ++  D K KIRRA EA GIP+T+V++  L  +F+  L  P   +   ++V I G G+ KAV N E+DVA +TIKA DDPR  N+ L I+PP NI S  DLVS WE+  G TL+  ++SE++++K
Sbjct:   65 ELSEHDKLVAVFKEVDIVISTLAVPQYLEQLKVIEAIKEAGNIKRFVPSEFGNEVDRVR-ALPRFQAVLDNKKKIRRATEAAGIPFTFVSANSLTAYFVDYLLHPRQKS---EQVTIYGSGDAKAVLNYEEDVAAYTIKAADDPRAANRVLIIKPPKNIVSQLDLVSSWEKTTGSTLKMTHISEQEIIK 249          
BLAST of CX300464 vs. ExPASy Swiss-Prot
Match: EGS1_OCIBA (Eugenol synthase 1 OS=Ocimum basilicum GN=EGS1 PE=1 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 2.806e-37
Identity = 80/189 (42.33%), Postives = 121/189 (64.02%), Query Frame = 1
Query:   13 HESLVKAIKQVDVVISTVGHTLIADQVKIIAAIKEAGNVKRFFPSEFGIDDDRIHGAVEPAKSTYDVKAKIRRAVEAEGIPYTYVASYGLNGHFLPNLSQPEATAPPRDKVVILGDGNPKAVYNKEDDVATFTIKAVDDPRTLNKNLYIQPPGNIYSFNDLVSMWERKIGKTLEREYVSEEQLLKNIQE 579
            HE LV+ +K+VDVVIS +    I DQ KI+ AIK AGN+KRF PS+FG+++DRI+ A+ P ++  + K  IRRA+E   IPYTYV++     +F+  L +P     P+D++ + G G  K   N E D+  +TIK   DPR LN+ +  +P  NI +  +L+S WE+KIGK  ++ +V EE+++   +E
Sbjct:   66 HEKLVELMKKVDVVISALAFPQILDQFKILEAIKVAGNIKRFLPSDFGVEEDRIN-ALPPFEALIERKRMIRRAIEEANIPYTYVSANCFASYFINYLLRPY---DPKDEITVYGTGEAKFAMNYEQDIGLYTIKVATDPRALNRVVIYRPSTNIITQLELISRWEKKIGKKFKKIHVPEEEIVALTKE 250          
The following BLAST results are available for this feature:
BLAST of CX300464 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
IFRH_SOLTU4.005e-7672.68Isoflavone reductase homolog OS=Solanum tuberosum ... [more]
IFRH_TOBAC4.438e-6765.80Isoflavone reductase homolog A622 OS=Nicotiana tab... [more]
IFRH_ARATH9.887e-6766.33Isoflavone reductase homolog P3 OS=Arabidopsis tha... [more]
IFR_MEDSA1.578e-6461.86Isoflavone reductase OS=Medicago sativa GN=IFR PE=... [more]
IFR_CICAR2.062e-6463.35Isoflavone reductase OS=Cicer arietinum GN=IFR PE=... [more]
IFR_PEA3.888e-6360.94Isoflavone reductase OS=Pisum sativum GN=IFR PE=2 ... [more]
IFRH_MAIZE6.012e-5657.73Isoflavone reductase homolog IRL OS=Zea mays GN=IR... [more]
IFRH_LUPAL6.438e-5049.23Isoflavone reductase homolog OS=Lupinus albus PE=2... [more]
IGS1_PETHY8.428e-4247.09Isoeugenol synthase 1 OS=Petunia hybrida GN=IGS1 P... [more]
EGS1_OCIBA2.806e-3742.33Eugenol synthase 1 OS=Ocimum basilicum GN=EGS1 PE=... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC07010E07SK SaltLeaf1 Citrus clementina cDNA clone C07010E07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX300464 ID=CX300464; Name=CX300464; organism=Citrus clementina; type=EST; length=583bp
GATGTTTTGAATCACGAGAGTTTGGTGAAGGCGATAAAACAAGTGGACGT
GGTGATATCAACAGTTGGTCACACTTTAATAGCCGATCAGGTCAAGATTA
TTGCTGCCATTAAAGAAGCCGGCAACGTTAAGAGATTCTTCCCGTCGGAA
TTTGGAATTGATGATGATCGAATCCATGGAGCCGTAGAGCCAGCAAAATC
AACGTATGACGTAAAGGCGAAAATCCGCCGAGCCGTTGAGGCAGAAGGCA
TTCCGTATACTTATGTAGCATCGTACGGTTTGAATGGCCACTTCCTTCCC
AATTTGTCGCAGCCTGAGGCCACTGCCCCCCCTAGGGATAAGGTCGTCAT
CTTAGGTGATGGAAATCCAAAAGCTGTTTATAACAAGGAAGATGATGTTG
CAACCTTCACCATCAAAGCGGTTGACGATCCGAGAACCTTGAACAAAAAC
CTTTACATTCAGCCTCCCGGCAATATTTACTCGTTCAATGATCTTGTGTC
TATGTGGGAGAGAAAGATTGGCAAAACCCTCGAAAGGGAGTATGTTTCAG
AAGAGCAGCTCCTAAAGAATATTCAAGAAGCTG
back to top