Developing fruit albedo at 80 DAFB in pTriplEx2 vector

Overview
Library NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
Unique NameDeveloping fruit albedo at 80 DAFB in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
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Feature NameUnique NameType
CK937473CK937473EST
CK937474CK937474EST
CK937475CK937475EST
CK937476CK937476EST
CK937477CK937477EST
CK937478CK937478EST
CK937479CK937479EST
CK937480CK937480EST
CK937481CK937481EST
CK937482CK937482EST
CK937483CK937483EST
CK937484CK937484EST
CK937485CK937485EST
CK937486CK937486EST
CK937487CK937487EST
CK937488CK937488EST
CK937489CK937489EST
CK937490CK937490EST
CK937491CK937491EST
CK937492CK937492EST
CK937493CK937493EST
CK937494CK937494EST
CK937495CK937495EST
CK937496CK937496EST
CK937497CK937497EST

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Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageDeveloping fruit albedo-collected July 29, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on July 29, 2003, between 8 to 9 am and stored at 4C. The albedo tissue was dissected out of developing fruit (80 DAFB) and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type