Flavedo Mature

Overview
Library NameFlavedo Mature
Unique NameFlavedo Mature
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Flavedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
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Feature NameUnique NameType
CB304429CB304429EST
CB304430CB304430EST
CB304431CB304431EST
CB304432CB304432EST
CB304433CB304433EST
CB304434CB304434EST
CB304435CB304435EST
CB304436CB304436EST
CB304437CB304437EST
CB304438CB304438EST
CB304439CB304439EST
CB304440CB304440EST
CB304441CB304441EST
CB304442CB304442EST
CB304443CB304443EST
CB304444CB304444EST
CB304445CB304445EST
CB304446CB304446EST
CB304447CB304447EST
CB304448CB304448EST
CB304449CB304449EST
CB304450CB304450EST
CB304451CB304451EST
CB304452CB304452EST
CB304453CB304453EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Flavedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Pericarptissue type