Construction of High-Density Genetic Maps and Detection of QTLs Associated With Huanglongbing Tolerance in Citrus.

Publication Overview
TitleConstruction of High-Density Genetic Maps and Detection of QTLs Associated With Huanglongbing Tolerance in Citrus.
AuthorsHuang M, Roose ML, Yu Q, Du D, Yu Y, Zhang Y, Deng Z, Stover E, Gmitter FG
TypeJournal Article
Journal NameFrontiers in plant science
Volume9
Year2018
Page(s)1694
CitationHuang M, Roose ML, Yu Q, Du D, Yu Y, Zhang Y, Deng Z, Stover E, Gmitter FG. Construction of High-Density Genetic Maps and Detection of QTLs Associated With Huanglongbing Tolerance in Citrus.. Frontiers in plant science. 2018; 9:1694.

Abstract

Huanglongbing (HLB), or citrus greening, is the most devastating disease in citrus worldwide. Commercial citrus varieties including sweet orange (Citrus sinensis) are highly susceptible to HLB, and trifoliate orange (Poncirus trifoliata, a close Citrus relative) is widely considered resistant or highly tolerant to HLB. In this study, an intergeneric F1 population of sweet orange and trifoliate orange was genotyped by Genotyping-by-Sequencing, and high-density SNP-based genetic maps were constructed separately for trifoliate orange and sweet orange. The two genetic maps exhibited high synteny and high coverage of the citrus genome. Progenies of the F1 population and their parents were planted in a replicated field trial, exposed to intense HLB pressure for 3 years, and then evaluated for susceptibility to HLB over 2 years. The F1 population exhibited a wide range in severity of HLB foliar symptom and canopy damage. Genome-wide QTL analysis based on the phenotypic data of foliar symptom and canopy damage in 2 years identified three clusters of repeatable QTLs in trifoliate orange linkage groups LG-t6, LG-t8 and LG-t9. Co-localization of QTLs for two traits was observed within all three regions. Additionally, one cluster of QTLs in sweet orange (linkage group LG-s7) was also detected. The majority of the identified QTLs each explained 18-30% of the phenotypic variation, indicating their major role in determining HLB responses. These results show, for the first time, a quantitative genetic nature yet the presence of major loci for the HLB tolerance in trifoliate orange. The results suggest that sweet orange also contains useful genetic factor(s) for improving HLB tolerance in commercial citrus varieties. Findings from this study should be very valuable and timely to researchers worldwide as they are hastily searching for genetic solutions to the devastating HLB crisis through breeding, genetic engineering, or genome editing.

Features
This publication contains information about 1,420 features:
Feature NameUniquenameType
1_260015061_26001506genetic_marker
1_262350531_26235053genetic_marker
1_265120351_26512035genetic_marker
1_266008491_26600849genetic_marker
1_269392291_26939229genetic_marker
1_270839491_27083949genetic_marker
1_273594411_27359441genetic_marker
1_27452541_2745254genetic_marker
1_276895791_27689579genetic_marker
1_279415641_27941564genetic_marker
1_281101661_28110166genetic_marker
1_282667981_28266798genetic_marker
1_285069851_28506985genetic_marker
1_28540841_2854084genetic_marker
1_288222341_28822234genetic_marker
1_30490661_3049066genetic_marker
1_36077221_3607722genetic_marker
1_4474331_447433genetic_marker
1_51276291_5127629genetic_marker
1_54754171_5475417genetic_marker
1_64501111_6450111genetic_marker
1_69155461_6915546genetic_marker
1_71172301_7117230genetic_marker
1_73673791_7367379genetic_marker
1_74194381_7419438genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Citrus-HLB_tolerance-Gmitter-2018
Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-C.sinensis-consensus-2018
Citrus-P.trifoliata-consensus-2018
Stocks
This publication contains information about 5 stocks:
Stock NameUniquenameType
C. sinensis_x_P. trifoliata-F1C. sinensis_x_P. trifoliata-F1population
Sanford_x_ArgentinaSanford_x_Argentinapopulation
Succari_x_Flying DragonSuccari_x_Flying Dragonpopulation
Flying Dragon_x_SuccariFlying Dragon_x_Succaripopulation
Large-flowerLarge-floweraccession
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1664-462X
pISSN1664-462X
Publication Date2018
Journal AbbreviationFront Plant Sci
DOI10.3389/fpls.2018.01694
Elocation10.3389/fpls.2018.01694
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland