DR405889

Overview
NameDR405889
Unique NameDR405889
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length698
Libraries
Library NameType
Mature Juice Sac from Navel Orange in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB1_NAEGR (Calcineurin subunit B OS=Naegleria gruberi GN=CNB1 PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 4.282e-23
Identity = 57/134 (42.54%), Postives = 91/134 (67.91%), Query Frame = 2
Query:  311 MGTASSMLTQYDIEEVQQHCHNLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVD-----GLNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGSFMS 697
            MGT +S L   ++EE+Q+  +  F+Q+EI  LY+RF +LD++  G IS DEFL +PE A+NPL +R++ + D      +NFK+F+A LSVF+A+   Q+K++  FKVYD D +G +S  ++  VL+ + G+ +S
Sbjct:    1 MGTNTSSLRPEEVEEMQKGTN--FTQKEIKKLYKRFKKLDKDGNGTISKDEFLMIPELAVNPLVKRVISIFDENGDGSVNFKEFIAALSVFNAQGDKQRKLEFAFKVYDIDGDGYISNGELFTVLKMMVGNNLS 132          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB1_DICDI (Calcineurin subunit B type 1 OS=Dictyostelium discoideum GN=cnbA PE=1 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.091e-18
Identity = 44/131 (33.59%), Postives = 85/131 (64.89%), Query Frame = 2
Query:  311 MGTASSMLTQYDIEEVQQHCHNLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVD-----GLNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            MG   S+L +  +E+++ +  + FS+ E+  LY+RF  LD++  G ++ DEFLS+P+ A+NPL +R++++ D      + F +FV  L+  S K + + K++ +F++YD DC+G +S  ++ +VL+ + G+
Sbjct:    1 MGNQHSLLNKEQLEQMKDN--SSFSEAELKKLYRRFQMLDKDGSGTLTTDEFLSIPDLALNPLLERVIQIFDQNKDNEIEFFEFVGTLATLSHKGTKEDKLKFLFQIYDIDCDGFISNGELFQVLKMMVGT 129          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB2_DICDI (Calcineurin subunit B type 2 OS=Dictyostelium discoideum GN=cnbB PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.425e-18
Identity = 50/131 (38.17%), Postives = 83/131 (63.36%), Query Frame = 2
Query:  311 MGTASSMLTQYDIEEVQQHCHNLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVD-----GLNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            MG + S L   +IEE+++   ++FSQ+EI  LY+RF +LD+  KG I+ ++F  +PE +MNP+  R++ + D      +NFK FV  LS F  KA    K++++FKVYD + +G ++ ++I  +L  + GS
Sbjct:    1 MGVSRSTLRTEEIEEIREV--SIFSQREIKRLYKRFKRLDKEEKGSINVEDFNQIPELSMNPMLPRIISIFDVNRDGQVNFKQFVKSLSTFHPKADKADKIKILFKVYDINNDGFITRDEIETILTMMVGS 129          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB_CANGA (Calcineurin subunit B OS=Candida glabrata GN=CNB1 PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.129e-15
Identity = 44/131 (33.59%), Postives = 79/131 (60.31%), Query Frame = 2
Query:  311 MGTASSMLTQYDIEEVQQHCHNLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVDG-----LNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            MG A S + +  +E+        F + EI  L +RF +LDR++ G I  +EF+S+P  + NPL+ R++++ D      ++F++F+  LS+FS + S  +K++  FK+YD D +G +S  ++  VL+ + GS
Sbjct:    1 MGAAPSKIVETLLEDTN------FDRDEIERLRKRFMKLDRDSSGSIDKNEFMSIPGVSANPLAGRIMEVFDADNSGDVDFQEFITGLSIFSGRGSKDEKLKFAFKIYDIDKDGLISNGELFIVLKIMVGS 125          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB_NEUCR (Calcineurin subunit B OS=Neurospora crassa GN=cnb-1 PE=2 SV=2)

HSP 1 Score: 82.4185 bits (202), Expect = 2.516e-15
Identity = 41/108 (37.96%), Postives = 72/108 (66.67%), Query Frame = 2
Query:  380 FSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVD-----GLNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            F ++E+  L +RF +LD++  G I  +EFLS+P+ + NPL+ R++ + D      ++F++FV+ LS FS+K + +QK++  FKVYD D +G +S  ++  VL+ + GS
Sbjct:   18 FDREEVDRLRKRFMKLDKDNSGTIEREEFLSLPQISTNPLATRMIAIFDEDGGGDVDFQEFVSGLSAFSSKGNKEQKLRFAFKVYDIDRDGYISNGELFIVLKMMVGS 125          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB_CRYNE (Calcineurin subunit B OS=Cryptococcus neoformans GN=CNB1 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.516e-15
Identity = 41/108 (37.96%), Postives = 68/108 (62.96%), Query Frame = 2
Query:  380 FSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVD-----GLNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            FS  E++ L +RF +LD++  G I  DEFL +P+ A NPL+ R++ + D      ++F++FV  LS FS+K    +K++  FKVYD D +G +S  ++  VL+ + G+
Sbjct:   18 FSGPELMRLKKRFMKLDKDGSGSIDKDEFLQIPQIANNPLAHRMIAIFDEDGSGTVDFQEFVGGLSAFSSKGGRDEKLRFAFKVYDMDRDGYISNGELYLVLKQMVGN 125          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB_YEAST (Calcineurin subunit B OS=Saccharomyces cerevisiae GN=CNB1 PE=1 SV=3)

HSP 1 Score: 82.0333 bits (201), Expect = 3.286e-15
Identity = 44/131 (33.59%), Postives = 78/131 (59.54%), Query Frame = 2
Query:  311 MGTASSMLTQYDIEEVQQHCHNLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVDG-----LNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            MG A S +    +E+        F + EI  L +RF +LDR++ G I  +EF+S+P  + NPL+ R++++ D      ++F++F+  LS+FS + S  +K++  FK+YD D +G +S  ++  VL+ + GS
Sbjct:    1 MGAAPSKIVDGLLEDTN------FDRDEIERLRKRFMKLDRDSSGSIDKNEFMSIPGVSSNPLAGRIMEVFDADNSGDVDFQEFITGLSIFSGRGSKDEKLRFAFKIYDIDKDGFISNGELFIVLKIMVGS 125          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB_ASHGO (Calcineurin subunit B OS=Ashbya gossypii GN=CNB1 PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.286e-15
Identity = 42/131 (32.06%), Postives = 83/131 (63.36%), Query Frame = 2
Query:  311 MGTASSMLTQYDIEEVQQHCHNLFSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVDG-----LNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            MG+A S + +  +++        F ++EI  L +RF +LDR++ G I  +EF+S+P  + NPL+ R++++ D      ++F++F++ LSVFS ++  ++K++  FK+YD D +G +S  ++  VL+ + G+
Sbjct:    1 MGSAPSKILEDLLDDTN------FDREEIERLRKRFMKLDRDSSGSIDKNEFMSIPGVSSNPLAGRIMEVFDADNSGDVDFQEFISGLSVFSGRSDKEEKLRFAFKIYDIDKDGYISNGELFIVLKIMVGN 125          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB_SCHPO (Calcineurin subunit B OS=Schizosaccharomyces pombe GN=cnb1 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.292e-15
Identity = 41/108 (37.96%), Postives = 68/108 (62.96%), Query Frame = 2
Query:  380 FSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVD-----GLNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            FS +EI  + +RF ++D N  G I  +EFLS+P  A NPL+ RL  +VD      ++F++F+  LSVFS   + ++K++  FK+YD D +G +S  ++  VL+ + G+
Sbjct:   18 FSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLASRLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGT 125          
BLAST of DR405889 vs. ExPASy Swiss-Prot
Match: CANB_YARLI (Calcineurin subunit B OS=Yarrowia lipolytica GN=CNB1 PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.249e-14
Identity = 42/108 (38.89%), Postives = 68/108 (62.96%), Query Frame = 2
Query:  380 FSQQEIVSLYQRFCQLDRNAKGFISADEFLSVPEFAMNPLSQRLLKMVD-----GLNFKDFVAFLSVFSAKASMQQKVQLIFKVYDSDCNGKVSFNDILEVLRDLSGS 688
            FS  EI  L +RF +LD +A G +  +EFLS+P  A NPL+ RL+ + D      ++F++F+  LS FS K + ++K++  FKVYD D +G +S  ++  VL+ + G+
Sbjct:   17 FSSDEIDRLRKRFMKLDTDASGILETNEFLSLPGVAANPLASRLMDVFDENHSGDVDFQEFINGLSTFSTKGNKKEKLRFAFKVYDIDRDGYISNGELFIVLKMMVGN 124          
The following BLAST results are available for this feature:
BLAST of DR405889 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
CANB1_NAEGR4.282e-2342.54Calcineurin subunit B OS=Naegleria gruberi GN=CNB1... [more]
CANB1_DICDI1.091e-1833.59Calcineurin subunit B type 1 OS=Dictyostelium disc... [more]
CANB2_DICDI1.425e-1838.17Calcineurin subunit B type 2 OS=Dictyostelium disc... [more]
CANB_CANGA1.129e-1533.59Calcineurin subunit B OS=Candida glabrata GN=CNB1 ... [more]
CANB_NEUCR2.516e-1537.96Calcineurin subunit B OS=Neurospora crassa GN=cnb-... [more]
CANB_CRYNE2.516e-1537.96Calcineurin subunit B OS=Cryptococcus neoformans G... [more]
CANB_YEAST3.286e-1533.59Calcineurin subunit B OS=Saccharomyces cerevisiae ... [more]
CANB_ASHGO3.286e-1532.06Calcineurin subunit B OS=Ashbya gossypii GN=CNB1 P... [more]
CANB_SCHPO4.292e-1537.96Calcineurin subunit B OS=Schizosaccharomyces pombe... [more]
CANB_YARLI1.249e-1438.89Calcineurin subunit B OS=Yarrowia lipolytica GN=CN... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCSAJ-PNP1248B11 Mature Juice Sac from Navel Orange in pTriplEx2 vector Citrus sinensis cDNA clone CSAJ-PNP1248B11 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR405889 ID=DR405889; Name=DR405889; organism=Citrus sinensis; type=EST; length=698bp
GGGGACGAGGAATATCCCACGTAATTCTCCGAAAATCAACGGCCGATAAA
ACGACGAAACATAAAAAAGAATGTTACTGCGTGACAGAGAATAATCGGTG
ATGAGGATGAACATCAGATTCCTCGTCATCTAGTACAAACAAAATACTAT
TCTGTGTGTTTTCAATGGCTCGATGATGACGAAGGAGTTTCTGTCATCTA
TATTTCCCGATACTTCATCTTCATCTAAACTCATCTCCTAGCGGTCGGTT
CTTTTTTTCCTACATTTTCTGTTTGTTTCCCGAGAAAACCCAGTTTTGGA
GCGAGAATCAATGGGGACTGCCTCGTCTATGCTAACTCAATATGATATTG
AAGAGGTTCAACAGCACTGCCACAATCTATTTTCTCAACAAGAAATAGTG
TCGTTGTACCAAAGGTTTTGCCAGCTAGATAGAAATGCCAAGGGATTCAT
CTCTGCAGATGAGTTCTTGTCGGTGCCTGAATTTGCAATGAATCCACTTT
CTCAGAGGTTGCTAAAGATGGTGGATGGGTTGAACTTCAAGGATTTTGTG
GCCTTCTTGTCTGTTTTCAGTGCTAAGGCAAGCATGCAGCAGAAAGTTCA
GCTTATTTTCAAAGTGTACGACTCAGACTGTAATGGAAAGGTTTCTTTCA
ATGACATATTGGAAGTGCTGCGGGATTTGTCTGGTTCTTTTATGTCCG
back to top