Carotenoid Biosynthetic Pathway in the Citrus Genus: Number of Copies and Phylogenetic Diversity of Seven Genes

Publication Overview
TitleCarotenoid Biosynthetic Pathway in the Citrus Genus: Number of Copies and Phylogenetic Diversity of Seven Genes
AuthorsFanciullino A, Dhuique-Mayer C, Luro F, Morillon R, Ollitrault P
TypeJournal Article
Journal NameJournal of agricultural and food chemistry
CitationFanciullino A, Dhuique-Mayer C, Luro F, Morillon R, Ollitrault P. Carotenoid Biosynthetic Pathway in the Citrus Genus: Number of Copies and Phylogenetic Diversity of Seven Genes. Journal of agricultural and food chemistry. 2007; 55(18):7405-7417.


The first objective of this paper was to analyze the potential role of allelic variability of carotenoid biosynthetic genes in the interspecific diversity in carotenoid composition of Citrus juices. The second objective was to determine the number of copies for each of these genes. Seven carotenoid biosynthetic genes were analyzed using restriction fragment length polymorphism (RFLP) and simple sequence repeats (SSR) markers. RFLP analyses were performed with the genomic DNA obtained from 25 Citrus genotypes using several restriction enzymes. cDNA fragments of Psy, Pds, Zds, Lcy-b, Lcy-e, Hy-b, and Zep genes labeled with [α-32P]dCTP were used as probes. For SSR analyses, two primer pairs amplifying two SSR sequences identified from expressed sequence tags (ESTs) of Lcy-b and Hy-b genes were designed. The number of copies of the seven genes ranged from one for Lcy-b to three for Zds. The genetic diversity revealed by RFLP and SSR profiles was in agreement with the genetic diversity obtained from neutral molecular markers. Genetic interpretation of RFLP and SSR profiles of four genes (Psy1, Pds1, Lcy-b, and Lcy-e1) enabled us to make inferences on the phylogenetic origin of alleles for the major commercial citrus species. Moreover, the results of our analyses suggest that the allelic diversity observed at the locus of both of lycopene cyclase genes, Lcy-b and Lcy-e1, is associated with interspecific diversity in carotenoid accumulation in Citrus. The interspecific differences in carotenoid contents previously reported to be associated with other key steps catalyzed by PSY, HY-b, and ZEP were not linked to specific alleles at the corresponding loci.
This publication contains information about 24 stocks:
Stock NameUniquenameType
Bouquetier de NiceBouquetier de Nicebreeding_research_material
Cara CaraCara Caracultivar
Deep redDeep redcultivar
Eureka frostEureka frostcultivar
Huang pi ChenHuang pi Chenbreeding_research_material
Mexican limeMexican limebreeding_research_material
Morocco sour orangeMorocco sour orangebreeding_research_material
Myrtle-leaf orangeMyrtle-leaf orangebreeding_research_material
Palestine sweet limePalestine sweet limebreeding_research_material
Ray RubyRay Rubycultivar
Seedless pummeloSeedless pummelobreeding_research_material
Volkamer lemonVolkamer lemoncultivar
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2007
Published Location|||
Language Abbreng
Publication Model[electronic resource].
KeywordsCitrus, genes, enzymes, carotenoids, biosynthesis, alleles, genetic variation, restriction fragment length polymorphism, microsatellite repeats, genetic markers, phylogeny, species differences
Cross References
This publication is also available in the following databases:
AGL: USDA National Agricultural LibraryAGL:4013964
PMID: PubMedPMID:17691802