Comparison between Poncirus and Citrus genetic linkage maps

Publication Overview
TitleComparison between Poncirus and Citrus genetic linkage maps
AuthorsRuiz C, Asins M
TypeJournal Article
Journal NameTheoretical and applied genetics
Volume106
Issue5
Year2003
Page(s)826-836
CitationRuiz C, Asins M. Comparison between Poncirus and Citrus genetic linkage maps. Theoretical and applied genetics. 2003 Mar; 106(5):826-836.

Abstract

Five genetic linkage maps were constructed for the parents of three progenies: Citrus aurantium (A) X Poncirus trifoliata var. Flying Dragon (Pa), C. volkameriana (V) X P. trifoliata var. Rubidoux (Pv) and a self-pollination of P. trifoliata var. Flying Dragon (Pp). The number of polymorphic markers assayed ranged from 48 for Pa to 120 for A according to the heterozygosity of each parental. As our focus was on genome comparison, most of the markers were newly generated simple sequence repeats. Inter-retrotransposon amplified polymorphisms based on four retrotransposon sequences isolated from Citrus spp were also used to saturate the maps. These polymorphisms were much more frequent in A (53) than in Pa (15) and randomly distributed throughout both genomes. Since comparative genomics and quantitative trait locus analysis applicability depends on the reliability of marker ordering, the causes of variation in marker order were investigated. Around 25% of the markers showed gametal segregation distortions. Segregation distortions were also observed at the zygotic level towards a reduction in the observed frequency of homozygotes from that expected in linkage groups 5 and 7. The presence of balanced lethal factors or gametal incompatibility genes in those genomic regions would explain a zygotic advantage of heterozygotes at these specific regions. Four differences in genomic organization were observed; three are putative translocations and affect homeologous linkage groups 3, 7 and 11, where highly distorted markers are found. Other causes of variation in marker order are also discussed: the introduction of new markers in the map, lowering the LOD score and the mapping software. These results represent the first comparative mapping analysis among Citrus and Poncirus species.
Stocks
This publication contains information about 7 stocks:
Stock NameUniquenameType
flying dragonflying dragoncultivar
Poncirus-FlyingDragon/FlyingDragon-RILPoncirus-FlyingDragon/FlyingDragon-RILpopulation
Afin vernaAfin vernacultivar
Citrus-AfinVerna/FlyingDragon-AfinVerna-F1Citrus-AfinVerna/FlyingDragon-AfinVerna-F1population
volkamerianavolkamerianacultivar
Citrus-Volkamer/Rubidoux-Volkamer-F1Citrus-Volkamer/Rubidoux-Volkamer-F1population
RubidouxRubidouxcultivar
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Language Abbreng
Publication Date2003 Mar
Journal AbbreviationTheor. appl. genet.
URLhttps://link.springer.com/article/10.1007/s00122-002-1095-x
KeywordsCitrus aurantium, Poncirus trifoliata, Citrus limonia, interspecific hybridization, chromosome mapping, linkage groups, genomics, genetic markers, microsatellite repeats, segregation distortion, gene segregation, retrotransposons, heterozygosity, chromosome translocation, inter-retrotransposon amplified polymorphism
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3595804
PMID: PubMedPMID:12647056