Developing fruit juice sac at 38 DAFB

Overview
Library NameDeveloping fruit juice sac at 38 DAFB
Unique NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK933866CK933866EST
CK933867CK933867EST
CK933868CK933868EST
CK933869CK933869EST
CK933870CK933870EST
CK933871CK933871EST
CK933872CK933872EST
CK933873CK933873EST
CK933874CK933874EST
CK933875CK933875EST
CK933876CK933876EST
CK933877CK933877EST
CK933878CK933878EST
CK933879CK933879EST
CK933880CK933880EST
CK933881CK933881EST
CK933882CK933882EST
CK933883CK933883EST
CK933884CK933884EST
CK933885CK933885EST
CK933886CK933886EST
CK933887CK933887EST
CK933888CK933888EST
CK933889CK933889EST
CK933890CK933890EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stage38 DAFB fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type