Mature Juice Sac from Navel Orange in pTriplEx2 vector

Overview
Library NameMature Juice Sac from Navel Orange in pTriplEx2 vector
Unique NameMature Juice Sac from Navel Orange in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
SNP Chip Base
Array NameMature Juice Sac from Navel Orange in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
DR406158DR406158EST
DR406159DR406159EST
DR406160DR406160EST
DR406161DR406161EST
DR406162DR406162EST
DR406163DR406163EST
DR406164DR406164EST
DR406165DR406165EST
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DR406168DR406168EST
DR406169DR406169EST
DR406170DR406170EST
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DR406172DR406172EST
DR406173DR406173EST
DR406174DR406174EST
DR406175DR406175EST
DR406176DR406176EST
DR406177DR406177EST
DR406178DR406178EST
DR406179DR406179EST
DR406180DR406180EST
DR406181DR406181EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Fruittissue type