Development and Characterization of Microsatellite Markers in Citrus

Publication Overview
TitleDevelopment and Characterization of Microsatellite Markers in Citrus
AuthorsAhmad R, Struss D, Southwick SM
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume128
Issue4
Year2003
Page(s)584-590
CitationAhmad R, Struss D, and Southwick SM. Development and Characterization of Microsatellite Markers in Citrus. Journal of the American Society for Horticultural Science. 2003. 128(4):584-590.

Abstract

We evaluated the potential of microsatellite markers for use in Citrus genome analysis. Microsatellite loci were identified by screening enriched and nonenriched libraries developed from `Washington Navel' Citrus. Microsatellite-containing clones were sequenced and 26 specific PCR primers were selected for cross-species amplification and identification of cultivars/clones in Citrus. After an enrichment procedure, on average 69.9% of clones contained dinucleotide repeats (CA)n and (CT)n, in contrast to <25% of the clones that were identified as positive in hybridization screening of a nonenriched library. A library enriched for trinucleotide (CTT)n contained <15% of the clones with (CTT)n repeats. Repeat length for most of the dinucleotide microsatellites was in the range of 10 to 30 units. We observed that enrichment procedure pulled out more of the (CA)n repeats than (CT)n repeats from the Citrus genome. All microsatellites were polymorphic except one. No correlation was observed between the number of alleles and the number of microsatellite repeats. In total, 118 putative alleles were detected using 26 primer pairs. The number of putative alleles per primer pair ranged from one to nine with an average of 4.5. Microsatellite markers discriminated sweet oranges [Citrus sinensis (L.) osb], mandarin (Citrus reticulata Blanco), grapefruit (Citrus paradisi Macf.), lemon [Citrus limon (L.) Burm.f.], and citrange (hybrids of trifoliate orange and sweet orange), at the species level, but individual cultivars/clones within sweet oranges, mandarins and grapefruit known to have evolved by somatic mutation remained undistinguishable. Since these microsatellite markers were conserved within different Citrus species, they could be used for linkage mapping, evolutionary and taxonomic study in Citrus.
Stocks
This publication contains information about 56 stocks:
Stock NameUniquenameType
FukumotoFukumotocultivar
GilleteGilletecultivar
Kawano Wase SatsumaKawano Wase Satsumacultivar
Lane LateLane Latecultivar
LisbonLisboncultivar
Marisol ClementineMarisol Clementinecultivar
MidknightMidknightcultivar
Monreal ClementineMonreal Clementinecultivar
MoroMorocultivar
NavelateNavelatecultivar
NavelinaNavelinacultivar
NewhallNewhallcultivar
Olinda (old line)Olinda (old line)cultivar
Olinda NucellarOlinda Nucellarcultivar
OroblancoOroblancobreeding_research_material
Oroval ClementineOroval Clementinecultivar
Parent Washington at LindcoveParent Washington at Lindcovecultivar
Parent Washington at UCRParent Washington at UCRcultivar
Parent Washington HistoricParent Washington Historiccultivar
Parent Washington Mulholland Nursery 1965Parent Washington Mulholland Nursery 1965cultivar
Parent Washington Mulholland Nursery 1972Parent Washington Mulholland Nursery 1972cultivar
Parent Washington Mulholland Nursery 1993Parent Washington Mulholland Nursery 1993cultivar
Parent Washington Z citrus NurseryParent Washington Z citrus Nurserycultivar
Powell LatePowell Latecultivar
Red blushRed blushcultivar

Pages

Features
This publication contains information about 26 features:
Feature NameUniquenameType
CMS-10CMS-10genetic_marker
CMS-14CMS-14genetic_marker
CMS-16CMS-16genetic_marker
CMS-17CMS-17genetic_marker
CMS-18CMS-18genetic_marker
CMS-19CMS-19genetic_marker
CMS-2CMS-2genetic_marker
CMS-20CMS-20genetic_marker
CMS-21CMS-21genetic_marker
CMS-23CMS-23genetic_marker
CMS-24CMS-24genetic_marker
CMS-26CMS-26genetic_marker
CMS-3CMS-3genetic_marker
CMS-30CMS-30genetic_marker
CMS-31CMS-31genetic_marker
CMS-33CMS-33genetic_marker
CMS-34CMS-34genetic_marker
CMS-39CMS-39genetic_marker
CMS-4CMS-4genetic_marker
CMS-45CMS-45genetic_marker
CMS-46CMS-46genetic_marker
CMS-47CMS-47genetic_marker
CMS-48CMS-48genetic_marker
CMS-6CMS-6genetic_marker
CMS-7CMS-7genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
URLhttp://journal.ashspublications.org/content/128/4/584.abstract
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4774903