Developing fruit juice sac at 38 DAFB

Overview
Library NameDeveloping fruit juice sac at 38 DAFB
Unique NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK933516CK933516EST
CK933517CK933517EST
CK933518CK933518EST
CK933519CK933519EST
CK933520CK933520EST
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CK933527CK933527EST
CK933528CK933528EST
CK933529CK933529EST
CK933530CK933530EST
CK933531CK933531EST
CK933532CK933532EST
CK933533CK933533EST
CK933534CK933534EST
CK933535CK933535EST
CK933536CK933536EST
CK933537CK933537EST
CK933538CK933538EST
CK933539CK933539EST
CK933540CK933540EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stage38 DAFB fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type