Flavedo Mature

Overview
Library NameFlavedo Mature
Unique NameFlavedo Mature
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Flavedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CB304755CB304755EST
CB304756CB304756EST
CB304757CB304757EST
CB304758CB304758EST
CB304759CB304759EST
CB304760CB304760EST
CB304761CB304761EST
CB304762CB304762EST
CB304763CB304763EST
CB304764CB304764EST
CB304765CB304765EST
CB304766CB304766EST
CB304767CB304767EST
CB304768CB304768EST
CB304769CB304769EST
CB304770CB304770EST
CB304771CB304771EST
CB304772CB304772EST
CB304773CB304773EST
CB304774CB304774EST
CB304775CB304775EST
CB304776CB304776EST
CB304777CB304777EST
CB304778CB304778EST
CB304779CB304779EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Flavedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Pericarptissue type