Flavedo Mature

Overview
Library NameFlavedo Mature
Unique NameFlavedo Mature
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Flavedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameFlavedo Mature
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CB304714CB304714EST
CB304713CB304713EST
CB304712CB304712EST
CB304711CB304711EST
CB304710CB304710EST
CB304709CB304709EST
CB304708CB304708EST
CB304707CB304707EST
CB304706CB304706EST
CB304705CB304705EST
CB304704CB304704EST
CB304703CB304703EST
CB304702CB304702EST
CB304701CB304701EST
CB304700CB304700EST
CB304699CB304699EST
CB304698CB304698EST
CB304697CB304697EST
CB304696CB304696EST
CB304695CB304695EST
CB304694CB304694EST
CB304693CB304693EST
CB304692CB304692EST
CB304691CB304691EST
CB304690CB304690EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Pericarptissue type
Genbank library noteOrgan: Peel/rind; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Flavedo tissue was separated from the rest of the peel and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.