A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers

Publication Overview
TitleA new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers
AuthorsGulsen O, Uzun A, Canan I, Seday U, Canihos E
TypeJournal Article
Journal NameEuphytica
Volume173
Issue2
Year2010
Page(s)265-277
CitationGulsen O, Uzun A, Canan I, Seday U, Canihos E. A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers. Euphytica. 2010; 173(2):265-277.

Abstract

Sequence-related amplified polymorphism (SRAP), simple sequence repeats (SSR), inter-simple sequence repeat (ISSR), peroxidase gene polymorphism (POGP), resistant gene analog (RGA), randomly amplified polymorphic DNA (RAPD), and a morphological marker, Alternaria brown spot resistance gene of citrus named as Cabsr caused by (Alternaria alternata f. sp. Citri) were used to establish genetic linkage map of citrus using a population of 164 F₁ individuals derived between ‘Clementine' mandarin (Citrus reticulata Blanco ‘Clementine) and ‘Orlando' tangelo' (C. paradisi Macf. ‘Duncan' × C. reticulata Blanco ‘Dancy'). A total of 609 markers, including 385 SRAP, 97 RAPD, 95 SSR, 18 ISSR, 12 POGP, and 2 RGA markers were used in linkage analysis. The ‘Clementine' linkage map has 215 markers, comprising 144 testcross and 71 intercross markers placed in nine linkage groups. The ‘Clementine' linkage map covered 858 cM with and average map distance of 3.5 cM between adjacent markers. The ‘Orlando' linkage map has 189 markers, comprising 126 testcross and 61 intercross markers placed in nine linkage groups. The ‘Orlando' linkage map covered 886 cM with an average map distance of 3.9 cM between adjacent markers. Segregation ratios for Cabsr were not significantly different from 1:1, suggesting that this trait is controlled by a single locus. This locus was placed in ‘Orlando' linkage group 1. The new map has an improved distribution of markers along the linkage groups with fewer gaps. Combining different marker systems in linkage mapping studies may give better genome coverage due to their chromosomal target site differences, therefore fewer gaps in linkage groups.
Features
This publication contains information about 614 features:
Feature NameUniquenameType
AG07AG07genetic_marker
AG14AG14genetic_marker
AT07AT07genetic_marker
ATC09ATC09genetic_marker
CA24CA24genetic_marker
CA27CA27genetic_marker
CA31CA31genetic_marker
CAA02CAA02genetic_marker
CaBsrCaBsrgenetic_marker
CAC15CAC15genetic_marker
CAC23CAC23genetic_marker
CAC33CAC33genetic_marker
CCA01CCA01genetic_marker
CMS04CMS04genetic_marker
CMS16CMS16genetic_marker
CMS30CMS30genetic_marker
CT02CT02genetic_marker
CT19CT19genetic_marker
CT21CT21genetic_marker
CX6F19CX6F19genetic_marker
Em10Me11aEm10Me11agenetic_marker
Em10Me11bEm10Me11bgenetic_marker
Em10Me13aEm10Me13agenetic_marker
Em10Me13bEm10Me13bgenetic_marker
Em10Me13cEm10Me13cgenetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-Orlando/Clementine-F1-Clementine-2010
Citrus-Orlando/Clementine-F1-Orlando-2010
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
DancyDancyaccession
DuncanDuncanaccession
OrlandoOrlandoaccession
ClementineClementineaccession
Citrus-Orlando/Clementine-F1-ClementineCitrus-Orlando/Clementine-F1-Clementinepopulation
Citrus-Orlando/Clementine-F1-OrlandoCitrus-Orlando/Clementine-F1-Orlandopopulation
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2010
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s10681-010-0146-7
KeywordsCitrus reticulata, clementines, genetic markers, microsatellite repeats, genetic polymorphism, peroxidase, random amplified polymorphic DNA technique, Alternaria alternata, plant pathogenic fungi, fungal diseases of plants, disease resistance, genetic resistance, linkage (genetics), chromosome mapping, Citrus paradisi, tangelos, interspecific hybridization, linkage groups, gene segregation, genome, sequence related amplified polymorphism, resistance genes, resistant gene analog, molecular sequence data