Ruby Orange Ovary at Anthesis cDNA Library

Overview
Library NameRuby Orange Ovary at Anthesis cDNA Library
Unique NameRuby Orange Ovary at Anthesis cDNA Library
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Ovaries at anthesis were collected by Federici in March 2003. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised 450,000 pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Wing, Yu). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Features
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Feature NameUnique NameType
CF835231CF835231EST
CF835230CF835230EST
CF835229CF835229EST
CF835228CF835228EST
CF835227CF835227EST
CF835226CF835226EST
CF835225CF835225EST
CF835224CF835224EST
CF835223CF835223EST
CF835222CF835222EST
CF835221CF835221EST
CF835220CF835220EST
CF835218CF835218EST
CF835217CF835217EST
CF835216CF835216EST
CF835215CF835215EST
CF835214CF835214EST
CF835213CF835213EST
CF835212CF835212EST
CF835211CF835211EST
CF835210CF835210EST
CF835209CF835209EST
CF835208CF835208EST
CF835207CF835207EST
CF835206CF835206EST

Pages

Properties
Property NameValue
Genbank library cultivarRuby
Genbank library dev stage20 year old trees
Genbank library noteVector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Ovaries at anthesis were collected by Federici in March 2003. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised 450,000 pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Wing, Yu). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Genbank library tissue typeOvary
Ovarytissue type