Developing fruit peel at 38 DAFB

Overview
Library NameDeveloping fruit peel at 38 DAFB
Unique NameDeveloping fruit peel at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CK934044CK934044EST
CK934045CK934045EST
CK934046CK934046EST
CK934047CK934047EST
CK934048CK934048EST
CK934049CK934049EST
CK934050CK934050EST
CK934051CK934051EST
CK934052CK934052EST
CK934053CK934053EST
CK934054CK934054EST
CK934055CK934055EST
CK934056CK934056EST
CK934057CK934057EST
CK934058CK934058EST
CK934059CK934059EST
CK934060CK934060EST
CK934061CK934061EST
CK934062CK934062EST
CK934063CK934063EST
CK934064CK934064EST
CK934065CK934065EST
CK934066CK934066EST
CK934067CK934067EST
CK934068CK934068EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type