Mature Juice Sac from Navel Orange in pTriplEx2 vector

Overview
Library NameMature Juice Sac from Navel Orange in pTriplEx2 vector
Unique NameMature Juice Sac from Navel Orange in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
SNP Chip Base
Array NameMature Juice Sac from Navel Orange in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
DR406105DR406105EST
DR406104DR406104EST
DR406103DR406103EST
DR406102DR406102EST
DR406101DR406101EST
DR406100DR406100EST
DR406099DR406099EST
DR406098DR406098EST
DR406097DR406097EST
DR406096DR406096EST
DR406095DR406095EST
DR406094DR406094EST
DR406093DR406093EST
DR406092DR406092EST
DR406091DR406091EST
DR406090DR406090EST
DR406089DR406089EST
DR406088DR406088EST
DR406087DR406087EST
DR406086DR406086EST
DR406085DR406085EST
DR406084DR406084EST
DR406083DR406083EST
DR406082DR406082EST
DR406081DR406081EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Fruittissue type