Developing fruit 24 DAFB

Overview
Library NameDeveloping fruit 24 DAFB
Unique NameDeveloping fruit 24 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit 24 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK935179CK935179EST
CK935180CK935180EST
CK935181CK935181EST
CK935182CK935182EST
CK935183CK935183EST
CK935184CK935184EST
CK935185CK935185EST
CK935186CK935186EST
CK935187CK935187EST
CK935188CK935188EST
CK935189CK935189EST
CK935190CK935190EST
CK935191CK935191EST
CK935192CK935192EST
CK935193CK935193EST
CK935194CK935194EST
CK935195CK935195EST
CK935196CK935196EST
CK935197CK935197EST
CK935198CK935198EST
CK935199CK935199EST
CK935200CK935200EST
CK935201CK935201EST
CK935202CK935202EST
CK935203CK935203EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 3 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 3, 2003, between 8 to 9 am and stored at 4C. The entire fruit was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type