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Overview
| Library Name | Developing fruit juice sac at 38 DAFB |
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| Unique Name | Developing fruit juice sac at 38 DAFB |
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| Organism | Citrus sinensis (Sweet orange) |
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| Type | cdna_library |
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Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
| Array Name | Developing fruit juice sac at 38 DAFB |
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| Organism | Citrus sinensis (Sweet orange) |
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| Type | cdna_library |
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Features
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Properties
| Property Name | Value |
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| Genbank library cultivar | Washington navel orange |
| Genbank library dev stage | 38 DAFB fruit sample-collected June 17, 2003 |
| Genbank library note | Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes. |
| Fruit | tissue type |
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