Washington Navel orange cold acclimated flavedo & albedo cDNA library

Overview
Library NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
Unique NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the field at University of California, Riverside Agricultural Operations since 1983. The scion was Washington Navel orange and the rootstock Carizzo Citrange. Tissue from mature fruit was harvested at mid-day in January 2002 during a cold spell, when pre-dawn temperatures were approximately -2 to 2 degree C. Approximately 2 cm median sections of the rind were excised in the field from several fruits, then wrapped in aluminum foil and frozen quickly in dry ice. Total RNA was extracted using a phenol extraction procedure described in J. Japanaese Soc. Hort. Sci. (1996) 64:809-814. Poly(A) RNA was purified, a cDNA library was made, and 1 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside (Fenton). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3530 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
SNP Chip Base
Array NameWashington Navel orange cold acclimated flavedo & albedo cDNA library
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CB293828CB293828EST
CB293830CB293830EST
CB293831CB293831EST
CB293832CB293832EST
CB293833CB293833EST
CB293834CB293834EST
CB293835CB293835EST
CB293836CB293836EST
CB293837CB293837EST
CB293838CB293838EST
CB293839CB293839EST
CB293840CB293840EST
CB293841CB293841EST
CB293842CB293842EST
CB293843CB293843EST
CB293844CB293844EST
CB293845CB293845EST
CB293846CB293846EST
CB293847CB293847EST
CB293848CB293848EST
CB293849CB293849EST
CB293850CB293850EST
CB293851CB293851EST
CB293852CB293852EST
CB293853CB293853EST

Pages

Properties
Property NameValue
Pericarptissue type
Genbank library cultivarWashington navel
Genbank library dev stageMature fruit
Genbank library noteVector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the field at University of California, Riverside Agricultural Operations since 1983. The scion was Washington Navel orange and the rootstock Carizzo Citrange. Tissue from mature fruit was harvested at mid-day in January 2002 during a cold spell, when pre-dawn temperatures were approximately -2 to 2 degree C. Approximately 2 cm median sections of the rind were excised in the field from several fruits, then wrapped in aluminum foil and frozen quickly in dry ice. Total RNA was extracted using a phenol extraction procedure described in J. Japanaese Soc. Hort. Sci. (1996) 64:809-814. Poly(A) RNA was purified, a cDNA library was made, and 1 million primary lambda cDNA clones were in vivo excised to give a population of pBluescript SK(-) phagemids. All steps to this point were performed in the TJ Close lab at the University of California, Riverside (Fenton). Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3530 at the Arizona Genomics Institute, University of Arizona (Collura, Feuerbacher, Kim, Kudrna, Wing, Yu). Chromatogram files were transmitted to UC Riverside (by Yu), then processed at UC Riverside (by Wanamaker) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Genbank library tissue typeRind containing flavedo and albedo