Developing fruit juice sac at 38 DAFB

Overview
Library NameDeveloping fruit juice sac at 38 DAFB
Unique NameDeveloping fruit juice sac at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Features
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Feature NameUnique NameType
CK932689CK932689EST
CK932690CK932690EST
CK932691CK932691EST
CK932692CK932692EST
CK932693CK932693EST
CK932694CK932694EST
CK932695CK932695EST
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CK932697CK932697EST
CK932698CK932698EST
CK932699CK932699EST
CK932700CK932700EST
CK932701CK932701EST
CK932702CK932702EST
CK932703CK932703EST
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CK932706CK932706EST
CK932707CK932707EST
CK932708CK932708EST
CK932709CK932709EST
CK932710CK932710EST
CK932711CK932711EST
CK932712CK932712EST
CK932713CK932713EST

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Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stage38 DAFB fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The juice sac tissue was dissected out and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type