Mature Juice Sac from Navel Orange in pTriplEx2 vector

Overview
Library NameMature Juice Sac from Navel Orange in pTriplEx2 vector
Unique NameMature Juice Sac from Navel Orange in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
SNP Chip Base
Array NameMature Juice Sac from Navel Orange in pTriplEx2 vector
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
DR405930DR405930EST
DR405929DR405929EST
DR405928DR405928EST
DR405927DR405927EST
DR405926DR405926EST
DR405925DR405925EST
DR405924DR405924EST
DR405923DR405923EST
DR405922DR405922EST
DR405921DR405921EST
DR405920DR405920EST
DR405919DR405919EST
DR405918DR405918EST
DR405917DR405917EST
DR405916DR405916EST
DR405915DR405915EST
DR405914DR405914EST
DR405913DR405913EST
DR405912DR405912EST
DR405911DR405911EST
DR405910DR405910EST
DR405909DR405909EST
DR405908DR405908EST
DR405907DR405907EST
DR405906DR405906EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageMature fruit sample - collected January 2002
Genbank library noteOrgan: juice sac; Vector: pTriplEx2; Site_1: EcoRI; Site_2: XbaI; Mature citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected in January 2002, between 1 and 3 PM and stored at 4C. Juice sac tissue was separated from the rest of the fruit and used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The amplified library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 3 84 well dishes.
Fruittissue type