Developing fruit peel at 38 DAFB

Overview
Library NameDeveloping fruit peel at 38 DAFB
Unique NameDeveloping fruit peel at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Organ: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
SNP Chip Base
Array NameDeveloping fruit peel at 38 DAFB
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Features
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Feature NameUnique NameType
CK934422CK934422EST
CK934423CK934423EST
CK934424CK934424EST
CK934425CK934425EST
CK934426CK934426EST
CK934427CK934427EST
CK934428CK934428EST
CK934429CK934429EST
CK934430CK934430EST
CK934431CK934431EST
CK934432CK934432EST
CK934433CK934433EST
CK934434CK934434EST
CK934435CK934435EST
CK934436CK934436EST
CK934437CK934437EST
CK934438CK934438EST
CK934439CK934439EST
CK934440CK934440EST
CK934441CK934441EST
CK934442CK934442EST
CK934443CK934443EST
CK934444CK934444EST
CK934445CK934445EST
CK934446CK934446EST

Pages

Properties
Property NameValue
Genbank library cultivarWashington navel orange
Genbank library dev stageYoung fruit sample-collected June 17, 2003
Genbank library noteOrgan: Fruit; Vector: pTriplEx2; Site_1: SfiIA; Site_2: SfiIB; Developing citrus fruits were harvested from trees growing in the Citrus variety collection in the Wolfskill experimental orchard located in Winters, California (USA). Fruit was collected on June 17, 2003, between 8 to 9 am and stored at 4C. The entire peel (including both flavedo and albedo) was used to isolate RNA using Trizol reagent from Invitrogen. The cDNA Library was constructed using the SMART cDNA library Kit (Clontech). The primary library was en masse evicted and plasmid DNA containing the cDNA library was isolated from the resultant bacterial population. Plasmid DNA was then transformed into ultra competent E coli cells (XL10 Gold; Stratagene). Transformants were plated out on Q-trays (2000 cfu/tray), picked using a Qbot and archived in 384 well dishes.
Fruittissue type