Ruby Orange Developing Flower cDNA Library

Overview
Library NameRuby Orange Developing Flower cDNA Library
Unique NameRuby Orange Developing Flower cDNA Library
OrganismCitrus sinensis (Sweet orange)
Typecdna_library
Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Stum, Yost, Wing). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Features
The following browser provides a quick view for new visitors. Use the searching mechanism to find specific features.
Feature NameUnique NameType
CN181898CN181898EST
CN181899CN181899EST
CN181900CN181900EST
CN181901CN181901EST
CN181902CN181902EST
CN181903CN181903EST
CN181904CN181904EST
CN181905CN181905EST
CN181906CN181906EST
CN181907CN181907EST
CN181908CN181908EST
CN181909CN181909EST
CN181910CN181910EST
CN181911CN181911EST
CN181912CN181912EST
CN181913CN181913EST
CN181914CN181914EST
CN181915CN181915EST
CN181916CN181916EST
CN181917CN181917EST
CN181920CN181920EST
CN181921CN181921EST
CN181922CN181922EST
CN181923CN181923EST
CN181924CN181924EST

Pages

Properties
Property NameValue
Genbank library dev stage20 year old trees
Genbank library cultivarRuby
Genbank library noteVector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two trees with open-pollinated flowers in a mixed planting at UC Riverside Agricultural Operations were the source of tissue. Flower buds were collected by Federici (Roose lab) from January March 2003 in four groups: 1) no white petals, 2) white petals visible, bud less than 0.5 cm, 3) bud more than 0.5 cm but not open, 4) newly opened flowers. Tissues were snap frozen and then stored at -80C until further processing. Fenton (Close lab) purified RNA by the phenol method described in J. Japanese Soc. Hort. Sci. 1996. 64 (4): 809-814, purified poly(A) mRNA using a PolyATtrack mRNA Isolation System IV (Promega), produced a primary cDNA library using a lambda ZAP XR cDNA Synthesis Kit (Stratagene), then mass-excised one million pfu from the primary library to produce a phagemid population. Phagemids were plated, plasmid DNA purified, cDNA clones archived, and DNA sequences determined bi-directionally using an ABI3730 at the Arizona Genomics Institute, University of Arizona (Kim, Kudrna, Stum, Yost, Wing). Chromatogram files were downloaded by FTP to UC Riverside (by Close), then processed at UC Riverside (by Wanamaker, Close lab) using the HarvEST pipeline (http://harvest.ucr.edu) to remove vector and cloning oligo sequences and various contaminants, and to trim to a high quality region. Sequences that retained a phred 17 region of at least 100 bases were deposited to GenBank.
Genbank library tissue typeFlower
Flowertissue type